{"product_id":"tribioscience-human-il-12-il-23-p40-elisa-1-step-and-1hr","title":"Tribioscience Human IL-12\/IL-23 p40 ELISA; 1 step and 1hr.","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Human IL-12\/IL-23 p40 ELISA; 1 step and 1hr.\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003eInterleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-12\/IL-23 p40 is typically 5 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-CV is \u0026lt; 10%, the Inter-CV is \u0026lt; 12%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-12\/IL-23 P40.\u003c\/p\u003e\n\u003cp\u003eNo-cross reactivity:\u003c\/p\u003e\n\u003cp\u003eRecombinant mouse IL-12; Recombinant mouse IL-12\/IL-23 p40.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204-3-1024x225.png\" width=\"551\" height=\"121\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3269-3-1024x597.png\" width=\"550\" height=\"320\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/std-curve-1.png\" width=\"337\" height=\"249\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"EOP-readonly EOP\" style=\"color: rgb(0, 0, 0);\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"676\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"66\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"274\"\u003e\n\u003cstrong\u003eSTORAGE OF\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eOPENED\/\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Microplate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269A\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12\/IL-23 p40.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Standard\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269B\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e30 μL of Recombinant human IL-12\/IL-23 p40 protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269C\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e2.1 mL of human IL-12\/IL-23 p40 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"274\"\u003e \n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269D\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3269E\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1x96-well plate","offer_id":40932391780410,"sku":"TBS3269","price":446.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS3269.jpg?v=1751879860","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/tribioscience-human-il-12-il-23-p40-elisa-1-step-and-1hr","provider":"MSE Supplies","version":"1.0","type":"link"}