{"product_id":"tribioscience-human-gamma-h2ax-rh2ax-fast-elisa","title":"Tribioscience Human Gamma-H2AX\/rH2AX Fast ELISA","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Human Gamma-H2AX\/rH2AX Fast ELISA\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTribioscience’s Fast Human γ-H2AX ELISA is designed to quantitatively detect human γ-H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 312.5 to 20000 pg\/mL. The levels of human γ-H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human γ-H2AX protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human is typically 10 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV and the Inter-assay CV are ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay specifically recognizes Histone H2AX phosphorylated at S139.\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity with unphosphorylated recombinant human (rh) Histone H2AX, rhHistone Macro H2A1, and rhHistone Macro H2A2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-0.png\" alt=\"TBS3235-0\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/09\/TBS3265-1.jpg\" width=\"542\" height=\"270\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/09\/TBS3265-2.png\" width=\"333\" height=\"253\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman γ-H2AX Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS3265A\u003c\/td\u003e\n\u003ctd\u003e96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human γ-H2AX.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman γ-H2AX Standard\u003c\/td\u003e\n\u003ctd\u003eTBS3265B\u003c\/td\u003e\n\u003ctd\u003e100 µl of Recombinant human γ-H2AX (200 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3265C\u003c\/td\u003e\n\u003ctd\u003e2.1 ml of biotin- human γ-H2AX antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3265D\u003c\/td\u003e\n\u003ctd\u003e230 µl of streptavidin HRP (60x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3265E\u003c\/td\u003e\n\u003ctd\u003e25 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003eThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1x96-well plate","offer_id":40932360323130,"sku":"TBS3265","price":737.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS3265.jpg?v=1751878810","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/tribioscience-human-gamma-h2ax-rh2ax-fast-elisa","provider":"MSE Supplies","version":"1.0","type":"link"}