{"product_id":"tribioscience-hdac-fluorometric-assay-kit-tbs2060","title":"Tribioscience HDAC Fluorometric Assay Kit (TBS2060)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience HDAC Fluorometric Assay Kit (TBS2060)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan style=\"color: rgb(22, 22, 22);\"\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHistones are the chief protein components of chromatin. Modification of histone proteins through acetylation and deacetylation affects chromatin structure and regulates gene expression. Histone hyperacetylation is well correlated with increased transcription, whereas hypoacetylation correlates with transcriptional repression. Histone deacetylases (HDACs), catalyze the removal of acetyl groups from an N-acetyl lysine amino acid on a histone, generally resulting in transcriptional silencing. Histone deacetylases have been grouped into four classes. Class I (HDAC 1, 2, 3, 8) and Class II (HDAC 4, 5, 6, 7, and 9) are zinc-containing hydrolase enzymes. The third class of HDACs consists of the members of the sirtuin family of enzymes (Sir 1 to 7). Class IV includes only HDAC11. The TriboTM HDAC Activity Assay Kit provides a convenient, homogeneous procedure for measuring HDAC activity from various sources of enzyme. It provides quantification of HDAC activity using a fluorogenic substrate. In the HDAC assay, an acetylated substrate is incubated with HDAC-containing samples. Deacetylation of substrate sensitizes it to the HDAC developer, and generates the AMC (7-amino-4-methylcoumarin) fluorophore, which can be detected at Ex354nm\/Em442nm. The substrate included in the kit is cell-permeable, and the assay can measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. The kit also can be used for HTS of HDAC inhibitors with extracts or purified enzymes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFluorometric\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at –20™ protected from light. Shelf life: 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"100 Tests","offer_id":40942005092410,"sku":"TBS2060","price":280.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS2060.jpg?v=1752471483","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/tribioscience-hdac-fluorometric-assay-kit-tbs2060","provider":"MSE Supplies","version":"1.0","type":"link"}