{"product_id":"tribioscience-cytochrome-c-reductase-activity-assay-tbs2116","title":"Tribioscience Cytochrome C Reductase Activity Assay (TBS2116)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Cytochrome C Reductase Activity Assay (TBS2116)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit is designed to measure the NADPH cytochrome C reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER). Eukaryotic NADPH-Cytochrome C reductase (NADPH cytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which is the cytochrome P450 family of enzymes, responsible for xenobiotic metabolism.\u003c\/p\u003e\n\u003cp\u003eNADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eMeasuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics.\u003c\/li\u003e\n\u003cli\u003eDetection of ER during isolation and subcellular fractionation by density gradient separation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe kit can be used for 96-well plates or 384-well plates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccurate:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eAccurate detection of cytochrome C reductase activity in small size as 50 µL samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eJust load-Read. The kit can be used for a robust method.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTime-saving:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLess than 30 minutes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" height=\"142\" style=\"width: 76.6071%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9534%;\"\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 36.5285%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9534%;\"\u003eCytochrome C\u003c\/td\u003e\n\u003ctd style=\"width: 36.5285%;\"\u003e1.2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9534%;\"\u003eNADPH\u003c\/td\u003e\n\u003ctd style=\"width: 36.5285%;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9534%;\"\u003eCytochrome C Reductase Positive\u003c\/td\u003e\n\u003ctd style=\"width: 36.5285%;\"\u003e0.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 62.9534%;\"\u003eCytochrome C Oxidase Inhibitor\u003c\/td\u003e\n\u003ctd style=\"width: 36.5285%;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore kit at -20°C. Shelf life of 2 years. Protect from light.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eShip with blue ice.\u003c\/span\u003e\u003c\/p\u003e","brand":"Tribioscience","offers":[{"title":"100 Tests","offer_id":40943138996282,"sku":"TBS2116","price":407.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS2116.jpg?v=1752563857","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/tribioscience-cytochrome-c-reductase-activity-assay-tbs2116","provider":"MSE Supplies","version":"1.0","type":"link"}