{"product_id":"total-iron-binding-capacity-tibc-colorimetric-assay-kit-1","title":"Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit","description":"\u003ch2\u003eTotal Iron Binding Capacity (TIBC) Colorimetric Assay Kit\u003c\/h2\u003e\n\u003ctable width=\"503\" style=\"height: 48px; width: 554px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 149.1px;\" data-mce-style=\"height: 10px; width: 149.1px;\"\u003e\u003cstrong\u003eSKU #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 391.3px;\" data-mce-style=\"height: 10px; width: 391.3px;\"\u003eE-BC-K071-S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 149.1px;\" data-mce-style=\"height: 19px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 391.3px;\" data-mce-style=\"height: 19px; width: 391.3px;\"\u003eSpectrophotometer (520 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 149.1px;\" data-mce-style=\"height: 19px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 391.3px; height: 19px;\" data-mce-style=\"width: 391.3px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" style=\"font-size: 0.875rem; height: 298px; width: 555px;\" data-mce-style=\"font-size: 0.875rem; height: 298px; width: 555px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 131.087px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 410.312px;\"\u003e\u003cspan\u003eTIBC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 126.925px;\" data-mce-style=\"height: 19px; width: 126.925px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px;\" data-mce-style=\"width: 394.475px;\"\u003eSerum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 10px;\" data-mce-style=\"width: 126.925px; height: 10px;\"\u003e\u003cspan\u003eSensitivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 10px;\" data-mce-style=\"width: 394.475px; height: 10px;\"\u003e\u003cspan\u003e0.03 mg\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003eDetection Range\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e\u003cspan\u003e0.03-50 mg\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003eDetection Method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003eAssay type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e\u003cspan\u003eQuantitative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e\u003cspan\u003e50 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003ePrecision\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003eAverage inter-assay CV: 4.700% | Average intra-assay CV: 3.400%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 126.925px; height: 19px;\" data-mce-style=\"width: 126.925px; height: 19px;\"\u003e\u003cspan\u003eOther instruments required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e\u003cspan\u003eMicropipettor, Water bath, Vortex mixer, Centrifuge\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 131.087px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 410.312px;\"\u003e\u003cspan\u003eNormal saline (0.9% NaCl)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 126.925px;\" data-mce-style=\"height: 10px; width: 126.925px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 10px;\" data-mce-style=\"width: 394.475px; height: 10px;\"\u003e\u003cspan\u003e2-8℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 126.925px;\" data-mce-style=\"height: 19px; width: 126.925px;\"\u003eValid period\u003c\/td\u003e\n\u003ctd style=\"width: 394.475px; height: 19px;\" data-mce-style=\"width: 394.475px; height: 19px;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImages\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eR M Adel et al investigate the destructive effect of iron overload by silver nanocomposite. Total iron binding capacity (TIBC) in rat serum was determined using TIBC colorimetric assay kit (E-BC-K071-S).\u003c\/p\u003e\n\u003cp\u003eTable. Effect of iron sucrose administration and subsequent co-treatments of DFX with Tr-CA and Ag@Tr-CA on serum Fe, TIBC, ferritin and Tf levels in different groups. Mean ± S.E.M. and % of change.\u003c\/p\u003e\n\u003ctable width=\"835\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e\u003cstrong\u003eFe μmol\/1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e\u003cstrong\u003e% of change\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e\u003cstrong\u003eTIBC μmol\/1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e\u003cstrong\u003e% of change\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e\u003cstrong\u003eFerritin ng\/mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e\u003cstrong\u003e% of change\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e\u003cstrong\u003eTf ng\/mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e\u003cstrong\u003e% of change\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003cstrong\u003eGroup 1 (control)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e19.10 ± 0.64 \u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e___\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e51.18 ± 0.27\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e___\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e4.50 ± 0.12 \u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e___\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e10.60 ± 0.09 \u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e___\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003cstrong\u003eGroup 2 (iron sucrose)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e56.23\u003csup\u003ea\u003c\/sup\u003e ± 2.94\u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e194.40%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e87.70\u003csup\u003ea\u003c\/sup\u003e ± 1.60\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e71.36%\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e6.833\u003csup\u003ea\u003c\/sup\u003e ± 0.47\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e51.78%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e23.80\u003csup\u003ea\u003c\/sup\u003e ± 1.97\u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e124.53%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003cstrong\u003eGroup 3 (DFX)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e19.66\u003csup\u003eb\u003c\/sup\u003e ± 0.31\u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e2.93%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e60.40\u003csup\u003ea,b\u003c\/sup\u003e ± 1.33\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e18.01%\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e5.33\u003csup\u003eb\u003c\/sup\u003e ±0.26\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e18.44%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e14.30\u003csup\u003ea,b\u003c\/sup\u003e ± 0.40\u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e34.91%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003cstrong\u003eGroup 4 (DFX + Tr-CA)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e21.23\u003csup\u003eb\u003c\/sup\u003e ± 0.63\u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e11.15%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e60.43\u003csup\u003ea,b\u003c\/sup\u003e ± 2.52 \u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e18.07%\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e4.70\u003csup\u003eb\u003c\/sup\u003e ± 0.21 \u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e4.44%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e15.23\u003csup\u003ea,b\u003c\/sup\u003e ± 0.13\u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e43.68%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64.075px;\"\u003e\u003cstrong\u003eGroup 5 (DFX + Ag@Tr-CA)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 53.825px;\"\u003e20.37\u003csup\u003eb\u003c\/sup\u003e ± 0.47\u003c\/td\u003e\n\u003ctd style=\"width: 56.5px;\"\u003e6.65%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e56.93\u003csup\u003ea,b\u003c\/sup\u003e ± 2.42\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e11.23%\u003c\/td\u003e\n\u003ctd style=\"width: 53.65px;\"\u003e4.73\u003csup\u003eb\u003c\/sup\u003e ± 0.27\u003c\/td\u003e\n\u003ctd style=\"width: 52.1875px;\"\u003e5.11%\u003c\/td\u003e\n\u003ctd style=\"width: 54.5125px;\"\u003e16.43\u003csup\u003ea,b\u003c\/sup\u003e ± 0.92\u003c\/td\u003e\n\u003ctd style=\"width: 56.55px;\"\u003e55%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eBy ANOVA, P \u0026lt; 0.05, LSD Test. a = P \u0026lt; 0.05 vs Group 1. b = P \u0026lt; 0.05 vs Group 2. a \u0026amp; b = The mean difference is significant at the 0.05 level.\u003c\/p\u003e\n\u003cp\u003eTIBC was significantly changed after treated.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of Sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIt is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-50 mg\/L).\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe\u003cspan\u003e \u003c\/span\u003erecommended\u003cspan\u003e \u003c\/span\u003edilution factor for different samples is as follows (for reference only):\u003cbr\u003e\u003c\/p\u003e\n\u003ctable width=\"189\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e\u003cstrong\u003eDilution factor\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003eHuman serum\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003eRat serum\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003ePorcine serum\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003eRabbit serum\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003eChicken serum\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 295.212px;\"\u003e\u003cspan\u003eCynomolgus monkey serum\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 239.188px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: The diluent is \u003c\/span\u003e\u003cspan\u003edouble distilled water \u003c\/span\u003e\u003cspan\u003eor\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003enormal saline (0.9% NaCl).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eDetection Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eKit Components \u0026amp; Storage\u003c\/strong\u003e\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 171px;\" width=\"639\" data-mce-style=\"height: 171px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eSize (50 Assays)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 1\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\" data-mce-fragment=\"1\"\u003e100 mg\/L Iron Standard Stock Solution\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\" data-mce-fragment=\"1\"\u003e7 mL ×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 2\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\" data-mce-fragment=\"1\"\u003eChromogenic Agent A\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\" data-mce-fragment=\"1\"\u003ePowder × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 month shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 3\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\" data-mce-fragment=\"1\"\u003eChromogenic Agent B\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\" data-mce-fragment=\"1\"\u003ePowder × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 month shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 4\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\" data-mce-fragment=\"1\"\u003eChromogenic Agent C\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\" data-mce-fragment=\"1\"\u003e60 mL × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 67.2375px; height: 19px;\" data-mce-style=\"width: 67.2375px; height: 19px;\"\u003eReagent 5\u003c\/td\u003e\n\u003ctd style=\"width: 123.025px; height: 19px;\" data-mce-style=\"width: 123.025px; height: 19px;\"\u003eIron Absorbent\u003c\/td\u003e\n\u003ctd style=\"width: 102.925px; height: 19px;\" data-mce-style=\"width: 102.925px; height: 19px;\"\u003e50 mg × 50 vials\u003c\/td\u003e\n\u003ctd style=\"width: 123.625px; height: 19px;\" data-mce-style=\"width: 123.625px; height: 19px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eNote: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eParameter:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 66px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 66px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eMean (mg\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e3.50\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e24.60\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e44.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 10px;\" data-mce-style=\"width: 28.5584%; height: 10px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 10px;\" data-mce-style=\"width: 21.7611%; height: 10px;\"\u003e3.6\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 10px;\" data-mce-style=\"width: 23.7683%; height: 10px;\"\u003e3.2\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 10px;\" data-mce-style=\"width: 24.8175%; height: 10px;\"\u003e3.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 53px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 53px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 15px;\" data-mce-style=\"width: 28.5584%; height: 15px;\"\u003e\u003cstrong\u003eMean (mg\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 15px;\" data-mce-style=\"width: 21.7611%; height: 15px;\"\u003e3.50\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 15px;\" data-mce-style=\"width: 23.7683%; height: 15px;\"\u003e24.60\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 15px;\" data-mce-style=\"width: 24.8175%; height: 15px;\"\u003e44.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e4.5\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e4.7\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e4.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTake three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 100%.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 73px;\" data-mce-style=\"width: 100%; height: 73px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 20px;\" data-mce-style=\"width: 32.573%; height: 20px;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 20px;\" data-mce-style=\"width: 21.0311%; height: 20px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 20px;\" data-mce-style=\"width: 20.6662%; height: 20px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 20px;\" data-mce-style=\"width: 24.635%; height: 20px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 15px;\" data-mce-style=\"width: 32.573%; height: 15px;\"\u003e\u003cstrong\u003eExpected Conc. (mg\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 15px;\" data-mce-style=\"width: 21.0311%; height: 15px;\"\u003e13.8\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 15px;\" data-mce-style=\"width: 20.6662%; height: 15px;\"\u003e34.7\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 15px;\" data-mce-style=\"width: 24.635%; height: 15px;\"\u003e45\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 19px;\" data-mce-style=\"width: 32.573%; height: 19px;\"\u003e\u003cstrong\u003eObserved Conc. (mg\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 19px;\" data-mce-style=\"width: 21.0311%; height: 19px;\"\u003e13.7\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 19px;\" data-mce-style=\"width: 20.6662%; height: 19px;\"\u003e34.0\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 19px;\" data-mce-style=\"width: 24.635%; height: 19px;\"\u003e46.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 19px;\" data-mce-style=\"width: 32.573%; height: 19px;\"\u003e\u003cstrong\u003eRecovery rate (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 19px;\" data-mce-style=\"width: 21.0311%; height: 19px;\"\u003e99\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 19px;\" data-mce-style=\"width: 20.6662%; height: 19px;\"\u003e98\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 19px;\" data-mce-style=\"width: 24.635%; height: 19px;\"\u003e103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe analytical sensitivity of the assay is 0.03 mg\/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:\u003c\/p\u003e\n\u003ctable style=\"height: 77px; width: 540px;\" width=\"662\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 39px; width: 116.775px;\" data-mce-style=\"height: 39px; width: 116.775px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eConcentration (mg\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e5\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e10\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e20\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e30\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.65px;\" data-mce-style=\"height: 39px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e40\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 47.675px;\" data-mce-style=\"height: 39px; width: 47.675px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e50\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 116.775px;\" data-mce-style=\"height: 19px; width: 116.775px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAverage OD\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.001\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.130\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.253\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.515\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.755\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e1.034\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.675px;\" data-mce-style=\"height: 19px; width: 47.675px;\" data-mce-fragment=\"1\"\u003e1.283\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 116.775px;\" data-mce-style=\"height: 19px; width: 116.775px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAbsoluted OD\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 47.65px;\" data-mce-style=\"height: 19px; width: 47.65px;\" data-mce-fragment=\"1\"\u003e0.129\u003c\/td\u003e\n\u003ctd style=\"height: 19px; 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