{"product_id":"total-antioxidant-capacity-t-aoc-colorimetric-assay-kit-abts-enzyme-method","title":"Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)","description":"\u003ch2\u003eTotal Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)\u003c\/h2\u003e\n\u003ctable style=\"height: 48px; width: 554px;\" width=\"503\" data-mce-style=\"height: 48px; width: 554px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 149.1px;\" data-mce-style=\"height: 10px; width: 149.1px;\"\u003e\u003cstrong\u003eSKU #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 391.3px;\" data-mce-style=\"height: 10px; width: 391.3px;\"\u003eE-BC-K219-M\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"height: 17px; width: 149.1px;\" data-mce-style=\"height: 17px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 17px; width: 391.3px;\" data-mce-style=\"height: 17px; width: 391.3px;\"\u003eMicroplate reader (405-425 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 149.1px; height: 19px;\" data-mce-style=\"width: 149.1px; height: 19px;\"\u003e\u003cstrong\u003e\u003cb\u003eDetection method\u003c\/b\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 391.3px; height: 19px;\" data-mce-style=\"width: 391.3px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" style=\"font-size: 0.875rem; height: 321px; width: 555px;\" data-mce-style=\"font-size: 0.875rem; height: 321px; width: 555px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 398.425px; height: 19px;\"\u003eT-AOC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25px;\"\u003e\n\u003ctd style=\"height: 25px; width: 131.875px;\" data-mce-style=\"height: 25px; width: 131.875px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 25px;\" data-mce-style=\"width: 395.525px; height: 25px;\"\u003e\u003cspan\u003eSerum, plasma, urine, saliva, tissue, cells\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 10px;\" data-mce-style=\"width: 131.875px; height: 10px;\"\u003e\u003cspan\u003eSensitivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 10px;\" data-mce-style=\"width: 395.525px; height: 10px;\"\u003e\u003cspan\u003e0.047 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eDetection Range\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003e\u003cspan\u003e0.047-1.50 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eDetection Method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eAssay type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003eQuantitative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003e\u003cspan\u003e20 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003ePrecision\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003eAverage inter-assay CV: 4.100% | Average intra-assay CV: 2.200%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 21px;\" data-mce-style=\"width: 131.875px; height: 21px;\"\u003e\u003cspan\u003eOther instruments required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 21px;\" data-mce-style=\"width: 395.525px; height: 21px;\"\u003e\u003cspan\u003eMicropipettor\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 131.875px; height: 19px;\" data-mce-style=\"width: 131.875px; height: 19px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003e\u003cspan\u003eNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 60% Ethanol\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 131.875px;\" data-mce-style=\"height: 10px; width: 131.875px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 10px;\" data-mce-style=\"width: 395.525px; height: 10px;\"\u003e\u003cspan\u003eReagent 5:-20℃, Others: 2-8℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 131.875px;\" data-mce-style=\"height: 19px; width: 131.875px;\"\u003eValid period\u003c\/td\u003e\n\u003ctd style=\"width: 395.525px; height: 19px;\" data-mce-style=\"width: 395.525px; height: 19px;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImages\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eJ A Garcia-Partida et al investigate the effect of mango leaf extract for improving brain healthy situation. Total antioxidant capacity (T-AOC) in rat hippocampus was determined using T-AOC colorimetric assay kit (E-BC-K219-M).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg height=\"167\" width=\"231\" style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/file.elabscience.com\/image\/product\/biochemical_kits\/citation\/E-BC-K219-M-CI.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT-AOC was significantly increased in C-RIS Group (light green bar). (P\u0026lt;0.05)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of Sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIt is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.047-1.50 mmol\/L).\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe recommended dilution factor for different samples is as follows (for reference only):\u003c\/p\u003e\n\u003ctable width=\"306\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003e\u003cstrong\u003eSample type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e\u003cstrong\u003eDilution factor\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003e10% Rat brain tissue homogenization\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003e10% Rat liver tissue homogenization\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003e10% Rat kidney tissue homogenization\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003e10% Epipremnum aureum tissue homogenization\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003eHuman serum\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003eHuman saliva\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003eHuman urine\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 385.312px;\"\u003eRat serum\u003c\/td\u003e\n\u003ctd style=\"width: 149.087px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e \u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eNote: When the sample was water soluble, the diluent is PBS (0.01 M, pH 7.4); When the sample is insoluble, the diluent is 80% ethanol.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eDetection Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eKit Components \u0026amp; Storage\u003c\/strong\u003e\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"734\" style=\"height: 125px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 19px;\" data-mce-style=\"width: 74.25px; height: 19px;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 19px;\" data-mce-style=\"width: 135.45px; height: 19px;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 94.325px; height: 19px;\" data-mce-style=\"width: 94.325px; height: 19px;\"\u003e\u003cstrong\u003eSize 1(48 T)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e\u003cstrong\u003eSize 2(96 T)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 19px;\" data-mce-style=\"width: 114.438px; height: 19px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 39px;\" data-mce-style=\"width: 74.25px; height: 39px;\"\u003eReagent 1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 39px;\" data-mce-style=\"width: 135.45px; height: 39px;\"\u003eBuffer Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 94.325px; height: 39px;\" data-mce-style=\"width: 94.325px; height: 39px;\"\u003e12 mL × 1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 100.338px; height: 39px;\" data-mce-style=\"width: 100.338px; height: 39px;\"\u003e24 mL × 1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 39px;\" data-mce-style=\"width: 114.438px; height: 39px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 39px;\" data-mce-style=\"width: 74.25px; height: 39px;\"\u003eReagent 2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 39px;\" data-mce-style=\"width: 135.45px; height: 39px;\"\u003eABTS Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 94.325px; height: 39px;\" data-mce-style=\"width: 94.325px; height: 39px;\"\u003e0.5 mL ×1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 100.338px; height: 39px;\" data-mce-style=\"width: 100.338px; height: 39px;\"\u003e1 mL ×1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 39px;\" data-mce-style=\"width: 114.438px; height: 39px;\"\u003e-20℃, 12 months shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 39px;\" data-mce-style=\"width: 74.25px; height: 39px;\"\u003eReagent 3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 39px;\" data-mce-style=\"width: 135.45px; height: 39px;\"\u003eH2O2 Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 94.325px; height: 39px;\" data-mce-style=\"width: 94.325px; height: 39px;\"\u003e0.25 mL ×1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 100.338px; height: 39px;\" data-mce-style=\"width: 100.338px; height: 39px;\"\u003e0.5 mL ×1 vial\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 39px;\" data-mce-style=\"width: 114.438px; height: 39px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 74.25px; height: 19px;\"\u003eReagent 4\u003c\/td\u003e\n\u003ctd style=\"width: 117.4px; height: 19px;\"\u003ePeroxidase\u003c\/td\u003e\n\u003ctd style=\"width: 112.387px; height: 19px;\"\u003e0.1 mL ×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\"\u003e0.2 mL ×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 114.425px; height: 19px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 74.25px; height: 19px;\"\u003eReagent 5\u003c\/td\u003e\n\u003ctd style=\"width: 117.4px; height: 19px;\"\u003e5 mmol\/L Trolox Standard\u003c\/td\u003e\n\u003ctd style=\"width: 112.387px; height: 19px;\"\u003e0.3 mL ×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\"\u003e0.6 mL ×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 114.425px; height: 19px;\"\u003e-20℃, 12 months shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 19px;\" data-mce-style=\"width: 74.25px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 19px;\" data-mce-style=\"width: 135.45px; height: 19px;\"\u003eMicroplate\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 199.863px; text-align: center; height: 19px;\" colspan=\"2\" data-mce-style=\"width: 199.863px; text-align: center; height: 19px;\"\u003e96 wells\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 19px;\" data-mce-style=\"width: 114.438px; height: 19px;\"\u003eNo requirement\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.25px; height: 19px;\" data-mce-style=\"width: 74.25px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 135.45px; height: 19px;\" data-mce-style=\"width: 135.45px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 199.863px; height: 19px; text-align: center;\" colspan=\"2\" data-mce-style=\"width: 199.863px; height: 19px; text-align: center;\"\u003e2 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 114.438px; height: 19px;\" data-mce-style=\"width: 114.438px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eNote: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eParameter:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 48px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 48px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eMean (mmol\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e0.12\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e0.58\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e1.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 10px;\" data-mce-style=\"width: 28.5584%; height: 10px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 10px;\" data-mce-style=\"width: 21.7611%; height: 10px;\"\u003e2.5\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 10px;\" data-mce-style=\"width: 23.7683%; height: 10px;\"\u003e2.3\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 10px;\" data-mce-style=\"width: 24.8175%; height: 10px;\"\u003e1.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 53px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 53px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 15px;\" data-mce-style=\"width: 28.5584%; height: 15px;\"\u003e\u003cstrong\u003eMean (mmol\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 15px;\" data-mce-style=\"width: 21.7611%; height: 15px;\"\u003e0.12\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 15px;\" data-mce-style=\"width: 23.7683%; height: 15px;\"\u003e0.58\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 15px;\" data-mce-style=\"width: 24.8175%; height: 15px;\"\u003e1.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e4.0\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e3.9\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e4.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTake three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 102%.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 72px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 72px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eStandard 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eStandard 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eStandard 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 15px;\" data-mce-style=\"width: 28.5584%; height: 15px;\"\u003e\u003cstrong\u003eExpected Conc. (mmol\/L) \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 15px;\" data-mce-style=\"width: 21.7611%; height: 15px;\"\u003e0.15\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 15px;\" data-mce-style=\"width: 23.7683%; height: 15px;\"\u003e0.33\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 15px;\" data-mce-style=\"width: 24.8175%; height: 15px;\"\u003e0.75\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eObserved Conc. (mmol\/L) \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e0.2\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e0.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 34.9452%; height: 19px;\"\u003e\u003cstrong\u003eRecovery rate (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.4838%; height: 19px;\"\u003e101\u003c\/td\u003e\n\u003ctd style=\"width: 20.6661%; height: 19px;\"\u003e105\u003c\/td\u003e\n\u003ctd style=\"width: 22.8102%; height: 19px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe analytical sensitivity of the assay is 0.047 mmol\/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAs the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:\u003c\/p\u003e\n\u003ctable style=\"height: 38px;\" width=\"451\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 166.7px;\"\u003e\u003cstrong\u003eConcentration (mmol\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0.1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0.2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0.3\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0.4\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e\u003cstrong\u003e0.6\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.125px;\"\u003e\u003cstrong\u003e0.8\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 166.7px;\"\u003eAverage OD\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e1.921\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e1.735\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e1.555\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e1.392\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e1.224\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.0625px;\"\u003e0.873\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 48.125px;\"\u003e0.543\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Assays","offer_id":40393092497466,"sku":"E-BC-K219-M-500","price":559.95,"currency_code":"USD","in_stock":true},{"title":"96 Tests","offer_id":40393092530234,"sku":"E-BC-K219-M-96","price":224.95,"currency_code":"USD","in_stock":true},{"title":"48 Tests","offer_id":40393092563002,"sku":"E-BC-K219-M-48","price":168.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/total-antioxidant-capacity-t-aoc-colorimetric-assay-kit-abts-enzyme-method-500-assays-elabscience-biotechnology-cell-assays-21891131965498.jpg?v=1752500381","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/total-antioxidant-capacity-t-aoc-colorimetric-assay-kit-abts-enzyme-method","provider":"MSE Supplies","version":"1.0","type":"link"}