{"product_id":"rat-hmgb-1-high-mobility-group-protein-b1-clia-kit","title":"Rat HMGB-1 (High Mobility Group Protein B1) CLIA Kit","description":"\u003ch2\u003eRat HMGB-1 (High Mobility Group Protein B1) CLIA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 428px; height: 29.5938px;\" data-mce-style=\"width: 428px; height: 29.5938px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 251.734px; height: 19.5938px;\" data-mce-style=\"width: 251.734px; height: 19.5938px;\"\u003e\u003cspan\u003e12.50-800 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 252.656px;\" data-mce-style=\"width: 252.656px;\"\u003e\u003cspan\u003e7.50 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv id=\"dt_tab1\" class=\"dt01\"\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eProduct Details\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cstrong\u003eProperties\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" style=\"width: 100%; height: 313.5px;\" data-mce-style=\"width: 100%; height: 313.5px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003eSandwich-CLIA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003e12.50-800 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003e7.50 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.7812px;\" data-mce-style=\"height: 58.7812px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003eserum, plasma and other biological fluids; 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.7812px;\" data-mce-style=\"height: 58.7812px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003eThis kit recognizes Rat HMGB-1 in samples. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003eBoth intra-CV and inter-CV are \u0026lt; 15%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.7812px;\" data-mce-style=\"height: 58.7812px;\"\u003e\n\u003ctd style=\"width: 29.8893%;\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"width: 69.7417%;\"\u003eThis CLIA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003cspan\u003eThis CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HMGB-1. You can calculate the concentration of Rat HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eKit components \u0026amp; Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 529.031px;\" class=\"dtl_table2 table_line\" data-mce-style=\"width: 100%; height: 529.031px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 23.8007%; height: 19.5938px;\" width=\"33%\" data-mce-style=\"width: 23.8007%; height: 19.5938px;\"\u003e\u003cb\u003eItem\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 28.7823%; height: 19.5938px;\" data-mce-style=\"width: 28.7823%; height: 19.5938px;\"\u003e\u003cb\u003eSpecifications\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 46.679%; height: 19.5938px;\" data-mce-style=\"width: 46.679%; height: 19.5938px;\"\u003e\u003cb\u003eStorage\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eMicro CLIA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e96T: 8 wells ×12 strips\u003cbr\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003ctd style=\"width: 46.679%;\" rowspan=\"3\"\u003e-20℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e96T: 2 vials\u003cbr\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 78.375px;\" data-mce-style=\"height: 78.375px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003ctd style=\"width: 46.679%;\"\u003e-20℃(Protect from light), 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 46.679%;\" rowspan=\"4\"\u003e2-8°C, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.7812px;\" data-mce-style=\"height: 58.7812px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eSubstrate Reagent A\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 46.679%;\" rowspan=\"2\"\u003e2-8℃(Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eSubstrate Reagent B\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 vial, 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 46.679%;\" rowspan=\"3\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.9852%;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 28.7823%;\"\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"dt_tab2\" class=\"dt01\"\u003e\n\u003ch2\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2\u003e\u003cspan class=\"title\"\u003eTechnical Data\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HMGB-1 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HMGB-1 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e44.19\u003c\/td\u003e\n\u003ctd\u003e90.41\u003c\/td\u003e\n\u003ctd\u003e349.84\u003c\/td\u003e\n\u003ctd\u003e41.54\u003c\/td\u003e\n\u003ctd\u003e91.66\u003c\/td\u003e\n\u003ctd\u003e355.69\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e5.62\u003c\/td\u003e\n\u003ctd\u003e7.74\u003c\/td\u003e\n\u003ctd\u003e38.45\u003c\/td\u003e\n\u003ctd\u003e4.29\u003c\/td\u003e\n\u003ctd\u003e10.44\u003c\/td\u003e\n\u003ctd\u003e31.41\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e12.72\u003c\/td\u003e\n\u003ctd\u003e8.56\u003c\/td\u003e\n\u003ctd\u003e10.99\u003c\/td\u003e\n\u003ctd\u003e10.33\u003c\/td\u003e\n\u003ctd\u003e11.39\u003c\/td\u003e\n\u003ctd\u003e8.83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of Rat HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"width: 100%; height: 78.3752px;\" data-mce-style=\"width: 100%; height: 78.3752px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 41.8819%; height: 19.5938px;\" data-mce-style=\"width: 41.8819%; height: 19.5938px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 18.4502%; height: 19.5938px;\" data-mce-style=\"width: 18.4502%; height: 19.5938px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"width: 38.9299%; height: 19.5938px;\" data-mce-style=\"width: 38.9299%; height: 19.5938px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 41.8819%;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.4502%;\"\u003e101-117\u003c\/td\u003e\n\u003ctd style=\"width: 38.9299%;\"\u003e107\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 41.8819%;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.4502%;\"\u003e86-97\u003c\/td\u003e\n\u003ctd style=\"width: 38.9299%;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 41.8819%;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.4502%;\"\u003e94-107\u003c\/td\u003e\n\u003ctd style=\"width: 38.9299%;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eTarget Information\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"width: 99.9792%; height: 10px;\" data-mce-style=\"width: 99.9792%; height: 10px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 22.3299%; height: 19.5938px;\" data-mce-style=\"width: 22.3299%; height: 19.5938px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 77.3241%; height: 19.5938px;\" data-mce-style=\"width: 77.3241%; height: 19.5938px;\"\u003e\u003cspan\u003eHMGB1, HMG1, HMG3, SBP-1, Amphoterin\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd width=\"25%\" style=\"width: 22.3113%; height: 10px;\" data-mce-style=\"width: 22.3113%; height: 10px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"width: 77.4023%; height: 10px;\" data-mce-style=\"width: 77.4023%; height: 10px;\"\u003eCancer, Epigenetics and Nuclear Signaling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eAssay Procedures\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_01.png\" class=\"show\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_02.png\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/lct_08.png\" class=\"jiantou\"\u003e\n\u003cp\u003e2.Remove the liquid.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_03.png\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/lct_08.png\" class=\"jiantou\"\u003e\n\u003cp\u003e3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_04.png\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/lct_08.png\" class=\"jiantou\"\u003e\n\u003cp\u003e4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_05.png\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/lct_08.png\" class=\"jiantou\"\u003e\n\u003cp\u003e5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/clia\/lct_06.png\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/lct_08.png\" class=\"jiantou\"\u003e\n\u003cp\u003e6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40600912527418,"sku":"E-CL-R0348","price":761.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CL-R0348.jpg?v=1725269577","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/rat-hmgb-1-high-mobility-group-protein-b1-clia-kit","provider":"MSE Supplies","version":"1.0","type":"link"}