{"product_id":"non-esterified-free-fatty-acids-nefa-colorimetric-assay-kit","title":"Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit","description":"\u003ch2\u003eNon-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit\u003c\/h2\u003e\n\u003ctable width=\"503\" style=\"height: 48px; width: 554px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 219.163px;\" data-mce-style=\"height: 10px; width: 219.163px;\"\u003e\u003cstrong\u003eSKU #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 321.237px;\" data-mce-style=\"height: 10px; width: 321.237px;\"\u003eE-BC-K014\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 219.163px;\" data-mce-style=\"height: 19px; width: 219.163px;\"\u003e\u003cstrong\u003eDetection Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 321.237px;\" data-mce-style=\"height: 19px; width: 321.237px;\"\u003eSemi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 219.163px;\" data-mce-style=\"height: 19px; width: 219.163px;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 321.237px; height: 19px;\" data-mce-style=\"width: 321.237px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" style=\"font-size: 0.875rem; height: 170px; width: 555px;\" data-mce-style=\"font-size: 0.875rem; height: 298px; width: 555px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.1px; height: 19px;\" data-mce-style=\"width: 134.1px; height: 19px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e\u003cspan\u003eNEFA\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 134.1px;\" data-mce-style=\"height: 19px; width: 134.1px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e\u003cspan\u003eSerum, plasma, tissue homogenate, cells, cell supernatant\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.1px; height: 19px;\" data-mce-style=\"width: 134.1px; height: 19px;\"\u003e\u003cspan\u003eDetection Range\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e\u003cspan\u003e0.01-3.0 mmol\/L\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.1px; height: 19px;\" data-mce-style=\"width: 134.1px; height: 19px;\"\u003e\u003cspan\u003eDetection Method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.1px; height: 19px;\" data-mce-style=\"width: 134.1px; height: 19px;\"\u003e\u003cspan\u003eAssay type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eQuantitative\u003c\/span\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 46px;\"\u003e\n\u003ctd style=\"width: 134.1px; height: 46px;\" data-mce-style=\"width: 134.1px; height: 46px;\"\u003e\u003cspan\u003eOther instruments required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 46px;\" data-mce-style=\"width: 407.3px; height: 46px;\"\u003e\u003cspan\u003eNormal saline (0.9% NaCl)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 134.1px;\" data-mce-style=\"height: 10px; width: 134.1px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 10px;\" data-mce-style=\"width: 407.3px; height: 10px;\"\u003e\u003cspan\u003e2-8℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 134.1px;\" data-mce-style=\"height: 19px; width: 134.1px;\"\u003eValid period\u003c\/td\u003e\n\u003ctd style=\"width: 407.3px; height: 19px;\" data-mce-style=\"width: 407.3px; height: 19px;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eNEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"96 Tests","offer_id":40362268426298,"sku":"E-BC-K014-96","price":576.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-BC-K014.jpg?v=1712573379","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/non-esterified-free-fatty-acids-nefa-colorimetric-assay-kit","provider":"MSE Supplies","version":"1.0","type":"link"}