{"product_id":"mse-pro-human-tnfa-tumor-necrosis-factor-alpha-elisa-kit","title":"MSE PRO Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit","description":"\u003ch2\u003eMSE PRO™ Human TNFa (Tumor Necrosis Factor Alpha) ELISA Kit\u003c\/h2\u003e\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eProduct name\u003c\/td\u003e\n\u003ctd\u003eHuman TNFa (Tumor Necrosis Factor Alpha) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eReactivity\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\" width=\"208px\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003eTNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e6.4 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay length\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eCytokine; Tumor immunity; Infection immunity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eUniprot ID\u003c\/td\u003e\n\u003ctd\u003eP01375\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"MSE Supplies","offers":[{"title":"48T","offer_id":41000639987770,"sku":"EK0064242-48T","price":264.95,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":41000640020538,"sku":"EK0064242-96T","price":375.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/EK0064242.jpg?v=1757569533","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/mse-pro-human-tnfa-tumor-necrosis-factor-alpha-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}