{"product_id":"human-ep-beta-endorphin-elisa-kit","title":"Human β-EP(Beta-Endorphin) ELISA Kit","description":"\u003ch2\u003eHuman β-EP(Beta-Endorphin) ELISA Kit\u003c\/h2\u003e\n\u003ctable width=\"210\" style=\"width: 254px;\" data-mce-style=\"width: 254px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 125.028px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 115.497px;\" data-mce-style=\"width: 115.497px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 125.028px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\" data-mce-fragment=\"1\"\u003e\n\u003ctable style=\"font-size: 0.875rem;\" class=\"dtl_table2\" data-mce-style=\"font-size: 0.875rem;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eThis kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eThis ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003cthead data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth width=\"33%\"\u003e\u003cb\u003eItem\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eSpecifications\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eStorage\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd\u003e96T: 8 wells ×12 strips\u003cbr\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003ctd rowspan=\"3\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eReference Standard\u003c\/td\u003e\n\u003ctd\u003e96T: 2 vials\u003cbr\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003cbr\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003ctd\u003e-20℃(Protect from light), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃(Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e5 pieces\u003c\/td\u003e\n\u003ctd rowspan=\"3\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003ePrecision\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level β-EP were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level β-EP were tested on 3 different plates, 20 replicates in each plate.\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e45.50\u003c\/td\u003e\n\u003ctd\u003e153.00\u003c\/td\u003e\n\u003ctd\u003e435.30\u003c\/td\u003e\n\u003ctd\u003e46.70\u003c\/td\u003e\n\u003ctd\u003e158.10\u003c\/td\u003e\n\u003ctd\u003e426.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e2.90\u003c\/td\u003e\n\u003ctd\u003e7.50\u003c\/td\u003e\n\u003ctd\u003e20.00\u003c\/td\u003e\n\u003ctd\u003e2.80\u003c\/td\u003e\n\u003ctd\u003e6.60\u003c\/td\u003e\n\u003ctd\u003e13.60\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e6.37\u003c\/td\u003e\n\u003ctd\u003e4.90\u003c\/td\u003e\n\u003ctd\u003e4.59\u003c\/td\u003e\n\u003ctd\u003e6.00\u003c\/td\u003e\n\u003ctd\u003e4.17\u003c\/td\u003e\n\u003ctd\u003e3.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe recovery of β-EP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e90-107\u003c\/td\u003e\n\u003ctd\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e87-102\u003c\/td\u003e\n\u003ctd\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e84-97\u003c\/td\u003e\n\u003ctd\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eTarget Information\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"width: 546px; height: 39.2046px;\" data-mce-style=\"width: 528px; height: 35px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6023px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 130.824px; height: 19.6023px;\" data-mce-style=\"width: 112.116px; height: 19px;\"\u003eDatabase Links\u003c\/td\u003e\n\u003ctd style=\"width: 400.284px; height: 19.6023px;\" data-mce-style=\"width: 421.989px; height: 19px;\"\u003e\u003cspan\u003e SwissProt: P01189\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6023px;\"\u003e\n\u003ctd style=\"width: 130.824px; height: 19.6023px;\" data-mce-style=\"width: 108px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 400.284px;\"\u003eb-EP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 130.824px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"width: 400.284px;\"\u003e Cancer, Metabolism, Neuroscience, Signal transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40446197334074,"sku":"E-EL-H0572","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ElabscienceBiotechnology_72b2fc98-a402-4f28-8270-f07b74ae14da.jpg?v=1714218066","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/human-ep-beta-endorphin-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}