{"product_id":"cyanine7-labeling-kit-50-kd-filtration-tube","title":"Cyanine7 Labeling Kit (50 KD Filtration Tube)","description":"\u003ch2\u003eCyanine7 Labeling Kit (50 KD Filtration Tube)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Cyanine 7 Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechni\u003c\/strong\u003e\u003cstrong\u003ecal Information\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab2\"\u003e\n\u003ctable class=\"dtl_table2\" style=\"width: 100%; height: 925.61px;\" data-mce-style=\"width: 100%; height: 925.61px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 57.8594px;\" data-mce-style=\"height: 57.8594px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eTechnical Information\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003eElabscience\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eCyanine 7 Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003eProtein labeling\/Antibody labeling (\u003cspan\u003e50 KD Filtration tube\u003c\/span\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 327.594px;\" data-mce-style=\"height: 327.594px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003ePrinciple\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003e\u003cimg height=\"211\" width=\"616\" class=\"img-responsive\" src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/principle-cm.jpg\" alt=\"\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eLabeling group\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003eCyanine 7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 422.594px;\" data-mce-style=\"height: 422.594px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eSpectrum\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003e\u003cimg height=\"309\" width=\"536\" class=\"img-responsive\" src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/Cyanine%207.png\" alt=\"\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003e2~8°C\/-20°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 10.8097%;\" width=\"150\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd style=\"width: 88.9095%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"detail_section\"\u003e \u003c\/p\u003e\n\u003cp class=\"detail_section\"\u003e\u003cstrong\u003eCharacteristic\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e The whole process takes only 90 min.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConvenient: \u003c\/strong\u003eThe dye has been activated and can be used directly. Filtration\u003cbr\u003etube desalts without dialysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible use:\u003c\/strong\u003e It can be used for both micro-labeling and large-scale labeling,\u003cbr\u003eand can label 0.1-1 mg proteins each time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWater solubility:\u003c\/strong\u003e Cyanine 7 in this kit is water-soluble, and the fluorescence\u003cbr\u003echaracteristics of the dye can be well measured and maintained in aqueous\u003cbr\u003ebuffer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEssential Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 60.567%;\" data-mce-style=\"width: 60.567%;\"\u003eExcitation\/Absorption maximum (nm) \u003c\/td\u003e\n\u003ctd style=\"width: 39.028%;\" data-mce-style=\"width: 39.028%;\"\u003e750\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 60.567%;\" data-mce-style=\"width: 60.567%;\"\u003eEmission maximum (nm)\u003c\/td\u003e\n\u003ctd style=\"width: 39.028%;\" data-mce-style=\"width: 39.028%;\"\u003e773\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 60.567%;\" data-mce-style=\"width: 60.567%;\"\u003eMolar extinction coefficient ε (L⋅mol−1⋅cm−1)\u003c\/td\u003e\n\u003ctd style=\"width: 39.028%;\" data-mce-style=\"width: 39.028%;\"\u003e240600\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 60.567%;\" data-mce-style=\"width: 60.567%;\"\u003eCorrection coefficient of 280nm(CF280)\u003c\/td\u003e\n\u003ctd style=\"width: 39.028%;\" data-mce-style=\"width: 39.028%;\"\u003e0.04\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv id=\"dt_tab2\" class=\"dt01\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLabeling Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWithin a certain pH range, Cyanine 7 specifically reacts with primary amino groups (N-terminal and lysine residue side chains) to form a stable amide bond, so as to realize the coupling of Cyanine 7 with protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"title\"\u003e\u003cstrong\u003eMaterials Not Supplied\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003ePipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).\u003c\/li\u003e\n\u003cli\u003eUltraviolet spectrophotometer or nanodrop or multifunctional microplate reader.\u003c\/li\u003e\n\u003cli\u003e37\u003cspan\u003e°\u003c\/span\u003eC incubator.\u003c\/li\u003e\n\u003cli\u003eCentrifuge (centrifugal force up to 12,000×g).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe unopened kit can be stored at 2~8\u003cspan\u003e°\u003c\/span\u003eC for 1 year, and the dissolved Cyanine 7 can be stored at -20\u003cspan\u003e°\u003c\/span\u003eC or -80\u003cspan\u003e°\u003c\/span\u003eC for 1 week.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"dt01\"\u003e\n\u003col\u003e\n\u003cli\u003ePlease select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.\u003c\/li\u003e\n\u003cli\u003eCyanine 7 is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from\u003cbr\u003econdensing into the dye, it is necessary to equilibrate the dye to room before the experiment.\u003c\/li\u003e\n\u003cli\u003eThe kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.\u003c\/li\u003e\n\u003cli\u003eThe optimal molecular ratio of the dye and protein (150KD) recommended for labeling with this kit is 18:1, which can ensure that the dye can label the protein, and DOS is in the normal range, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"10 Reactions","offer_id":40459893473338,"sku":"E-LK-C005C-10","price":893.95,"currency_code":"USD","in_stock":true},{"title":"3 Reactions","offer_id":40459893506106,"sku":"E-LK-C005C-3","price":447.95,"currency_code":"USD","in_stock":true},{"title":"1 Reaction","offer_id":40459893538874,"sku":"E-LK-C005C-1","price":224.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/labeling_kit_d554c51e-dabd-42e8-beab-f0cff8bd99a7.jpg?v=1715081537","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/cyanine7-labeling-kit-50-kd-filtration-tube","provider":"MSE Supplies","version":"1.0","type":"link"}