{"product_id":"cyanine-3-labeling-kit-10-kd-filtration-tube","title":"Cyanine 3 Labeling Kit (10 KD Filtration Tube)","description":"\u003ch2\u003eCyanine 3 Labeling Kit (10 KD Filtration Tube)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"detail_section\"\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"detail_section\"\u003eElabscience® Cyanine 3 Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.\u003c\/p\u003e\n\u003cp class=\"detail_section\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechni\u003c\/strong\u003e\u003cstrong\u003ecal Information\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab1\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab2\"\u003e\n\u003ctable class=\"dtl_table2\" style=\"width: 100%; height: 531.062px;\" data-mce-style=\"width: 100%; height: 531.062px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 61.8625px;\" data-mce-style=\"height: 61.8625px;\"\u003e\n\u003ctd width=\"150\" style=\"width: 27.3723%; height: 61.8625px;\" data-mce-style=\"width: 27.3723%; height: 61.8625px;\"\u003eTechnical Information\u003c\/td\u003e\n\u003ctd style=\"width: 80.292%; height: 61.8625px;\" data-mce-style=\"width: 80.292%; height: 61.8625px;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eElabscience\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e Cyanine 3 Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"150\" style=\"width: 27.3723%; height: 19.6px;\" data-mce-style=\"width: 27.3723%; height: 19.6px;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 80.292%; height: 19.6px;\" data-mce-style=\"width: 80.292%; height: 19.6px;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eProtein labeling (\u003c\/span\u003e\u003cspan\u003e10 KD Filtration tube\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 160px;\" data-mce-style=\"height: 160px;\"\u003e\n\u003ctd width=\"150\" style=\"width: 27.3723%; height: 160px;\" data-mce-style=\"width: 27.3723%; height: 160px;\"\u003ePrinciple\u003c\/td\u003e\n\u003ctd style=\"width: 80.292%; height: 160px;\" data-mce-style=\"width: 80.292%; height: 160px;\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/principle-cm.jpg\" width=\"430\" height=\"147\" style=\"float: left;\" data-mce-style=\"float: left;\" data-mce-src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/principle-cm.jpg\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"150\" style=\"width: 27.3723%; height: 19.6px;\" data-mce-style=\"width: 27.3723%; height: 19.6px;\"\u003eLabeling group\u003c\/td\u003e\n\u003ctd style=\"width: 80.292%; height: 19.6px;\" data-mce-style=\"width: 80.292%; height: 19.6px;\"\u003eCyanine 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 270px;\" data-mce-style=\"height: 270px;\"\u003e\n\u003ctd width=\"150\" style=\"width: 27.3723%; height: 270px;\" data-mce-style=\"width: 27.3723%; height: 270px;\"\u003eSpectrum\u003c\/td\u003e\n\u003ctd style=\"width: 80.292%; height: 270px;\" data-mce-style=\"width: 80.292%; height: 270px;\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/Cyanine%203.png\" style=\"float: left;\" width=\"444\" height=\"256\" data-mce-style=\"float: left;\" data-mce-src=\"https:\/\/file.elabscience.com\/image\/product\/labeling_kits\/Cyanine%203.png\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 17.3049%; height: 19.6px;\" data-mce-style=\"width: 17.3049%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 82.4742%; height: 19.6px;\" data-mce-style=\"width: 82.4742%; height: 19.6px;\"\u003e2~8°C\/-20°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 17.3049%; height: 19.6px;\" data-mce-style=\"width: 17.3049%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 82.4742%;\" data-mce-style=\"width: 82.4742%;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.3049%;\" data-mce-style=\"width: 17.3049%;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 82.4742%;\" data-mce-style=\"width: 82.4742%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"detail_section\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCharacteristic\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e The whole process takes only 90 min.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConvenient:\u003c\/strong\u003e The dye has been activated and can be used directly. Filtration tube desalts without dialysis.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible use:\u003c\/strong\u003e It can be used for both micro-labeling and large-scale labeling, and can label 0.1-1 mg proteins each time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWater solubility:\u003c\/strong\u003e Cyanine 3 in this kit is water-soluble, and the fluorescence characteristics of the dye can be well measured and maintained in aqueous buffer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEssential Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 78.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 60.8694%; height: 19.6px;\"\u003eExcitation\/Absorption maximum (nm) \u003c\/td\u003e\n\u003ctd style=\"width: 38.9097%; height: 19.6px;\"\u003e548 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 60.8694%; height: 19.6px;\"\u003eEmission maximum (nm)\u003c\/td\u003e\n\u003ctd style=\"width: 38.9097%; height: 19.6px;\"\u003e563\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 60.8694%; height: 19.6px;\"\u003eMolar extinction coefficient ε (L⋅mol−1⋅cm−1)\u003c\/td\u003e\n\u003ctd style=\"width: 38.9097%; height: 19.6px;\"\u003e162000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 60.8694%; height: 19.6px;\"\u003eCorrection coefficient of 280nm(CF280)\u003c\/td\u003e\n\u003ctd style=\"width: 38.9097%; height: 19.6px;\"\u003e0.06\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLabeling Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWithin a certain pH range, Cyanine 3 specifically reacts with primary amino groups (N-terminal and lysine residue side chains) to form a stable amide bond, so as to realize the coupling of Cyanine 3 with protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials Not Supplied\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003ePipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).\u003c\/li\u003e\n\u003cli\u003eUltraviolet spectrophotometer or nanodrop or multifunctional microplate reader.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e37°C incubator.\u003c\/li\u003e\n\u003cli\u003eCentrifuge (centrifugal force up to 12,000×g).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe unopened kit can be stored at 2~8°C for 1 year, and the dissolved Cyanine 3 can be stored at -20°C or -80°C for 1 week.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv id=\"dt_tab2\" class=\"dt01\"\u003e\n\u003cp class=\"detail_section\"\u003e\u003cstrong\u003eNotes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003ePlease select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 10 KD Filtration tube.\u003c\/li\u003e\n\u003cli\u003eCyanine 3 is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the dye, it is necessary to equilibrate the dye to room before the experiment.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"10 Reactions","offer_id":40458650845242,"sku":"E-LK-C002B-10","price":893.95,"currency_code":"USD","in_stock":true},{"title":"3 Reactions","offer_id":40458650878010,"sku":"E-LK-C002B-3","price":447.95,"currency_code":"USD","in_stock":true},{"title":"1 Reaction","offer_id":40458650910778,"sku":"E-LK-C002B-1","price":224.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-LK-C002B.jpg?v=1714973492","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/cyanine-3-labeling-kit-10-kd-filtration-tube","provider":"MSE Supplies","version":"1.0","type":"link"}