{"product_id":"bradford-protein-colorimetric-assay-kit-1","title":"Bradford Protein Colorimetric Assay Kit","description":"\u003ch2\u003eBradford Protein Colorimetric Assay Kit\u003c\/h2\u003e\n\u003ctable style=\"height: 48px; width: 554px;\" width=\"503\" data-mce-style=\"height: 48px; width: 554px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 149.1px;\" data-mce-style=\"height: 10px; width: 149.1px;\"\u003e\u003cstrong\u003eSKU #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 391.3px;\" data-mce-style=\"height: 10px; width: 391.3px;\"\u003eE-BC-K168-S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"height: 17px; width: 149.1px;\" data-mce-style=\"height: 17px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 17px; width: 391.3px;\" data-mce-style=\"height: 17px; width: 391.3px;\"\u003eSpectrophotometer (595 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 149.1px; height: 19px;\" data-mce-style=\"width: 149.1px; height: 19px;\"\u003e\u003cstrong\u003e\u003cb\u003eDetection method\u003c\/b\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 391.3px; height: 19px;\" data-mce-style=\"width: 391.3px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" style=\"font-size: 0.875rem; height: 321px; width: 555px;\" data-mce-style=\"font-size: 0.875rem; height: 321px; width: 555px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003eTP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 25px;\"\u003e\n\u003ctd style=\"height: 25px; width: 134.875px;\" data-mce-style=\"height: 25px; width: 134.875px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 25px;\" data-mce-style=\"width: 406.525px; height: 25px;\"\u003e\u003cspan\u003eSerum, plasma, animal tissue\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 10px;\" data-mce-style=\"width: 134.875px; height: 10px;\"\u003e\u003cspan\u003eSensitivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 10px;\" data-mce-style=\"width: 406.525px; height: 10px;\"\u003e\u003cspan\u003e0.026 mg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eDetection Range\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406px;\"\u003e\u003cspan\u003e0.026-1.2 mg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eDetection Method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eAssay type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003eQuantitative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003e\u003cspan\u003e50 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003ePrecision\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003eAverage inter-assay CV: 2.300% | Average intra-assay CV: 1.800%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 21px;\" data-mce-style=\"width: 134.875px; height: 21px;\"\u003e\u003cspan\u003eOther instruments required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 21px;\" data-mce-style=\"width: 406.525px; height: 21px;\"\u003e\u003cspan\u003eMicropipettor, Vortex mixer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 134.875px; height: 19px;\" data-mce-style=\"width: 134.875px; height: 19px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 406px;\"\u003e\u003cspan\u003eNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 134.875px;\" data-mce-style=\"height: 10px; width: 134.875px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 10px;\" data-mce-style=\"width: 406.525px; height: 10px;\"\u003e\u003cspan\u003eReagent 1: 2-8℃, Reagent 2: RT\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 134.875px;\" data-mce-style=\"height: 19px; width: 134.875px;\"\u003eValid period\u003c\/td\u003e\n\u003ctd style=\"width: 406.525px; height: 19px;\" data-mce-style=\"width: 406.525px; height: 19px;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of Sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eIt is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg\/mL).\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eThe recommended dilution factor for different samples is as follows (for reference only):\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 76px;\" width=\"100%\" data-mce-fragment=\"1\" data-mce-style=\"width: 100%; height: 76px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 66.6058%; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2993%; height: 19px;\"\u003e\u003cspan\u003eDilution factor\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 66.6058%; height: 19px;\"\u003e\u003cspan\u003eHuman serum\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2993%; height: 19px;\"\u003e\u003cspan\u003e40-60\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 66.6058%; height: 19px;\"\u003e\u003cspan\u003e10% Mouse liver tissue homogenization\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2993%; height: 19px;\"\u003e8-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 66.6058%; height: 19px;\"\u003e\u003cspan\u003eRat serum\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.2993%; height: 19px;\"\u003e40-60\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eDetection Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCoomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eKit Components \u0026amp; Storage\u003c\/strong\u003e\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 221px;\" width=\"606\" data-mce-fragment=\"1\" data-mce-style=\"height: 221px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 36px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 74.4px; height: 36px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.4px; height: 36px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 112.6px; height: 36px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 112.6px; height: 36px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 109.588px; height: 36px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 109.588px; height: 36px;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 114.637px; height: 36px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 114.637px; height: 36px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 74.4px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.4px; height: 10px;\"\u003eReagent 1\u003c\/td\u003e\n\u003ctd style=\"width: 112.6px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 112.6px; height: 10px;\"\u003eChromogenic Agent Stock Solution\u003c\/td\u003e\n\u003ctd style=\"width: 109.588px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 109.588px; height: 10px;\"\u003e35 mL × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 114.637px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 114.637px; height: 10px;\"\u003e2-8℃, shading light,\u003cbr\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 40px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 40px;\"\u003e\n\u003ctd style=\"width: 74.4px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.4px; height: 10px;\"\u003eReagent 2\u003c\/td\u003e\n\u003ctd style=\"width: 112.6px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 112.6px; height: 10px;\"\u003e0.563 mg BSA Standard\u003c\/td\u003e\n\u003ctd style=\"width: 109.588px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 109.588px; height: 10px;\"\u003e0.563 mg × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 114.637px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 114.637px; height: 10px;\"\u003eRT, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eNote: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other.\u003c\/p\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Assays","offer_id":40387857121338,"sku":"E-BC-K168-S-500","price":224.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40387857154106,"sku":"E-BC-K168-S-100","price":113.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/bradford-protein-colorimetric-assay-kit-500-assays-elabscience-biotechnology-cell-assays-21887793528890.jpg?v=1752500621","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/bradford-protein-colorimetric-assay-kit-1","provider":"MSE Supplies","version":"1.0","type":"link"}