{"product_id":"annexin-v-elab-fluor-647-azide-free-lyophilized-powder","title":"Annexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of Elab Fluor® 647-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium dependent manner. The Elab Fluor® 647-conjugated format of this protein, Annexin V-Elab Fluor® 647, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-Elab Fluor® 647 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 647 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 647 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e 647\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e650\/670\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin V-Elab Fluor® 647 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Elab Fluor® 647. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003cbr\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40439244750906,"sku":"E-CK-A113U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40439244783674,"sku":"E-CK-A113U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40439244816442,"sku":"E-CK-A113U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40439244849210,"sku":"E-CK-A113U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A113U.jpg?v=1714025387","url":"https:\/\/www.msesupplies.com\/en-gb\/products\/annexin-v-elab-fluor-647-azide-free-lyophilized-powder","provider":"MSE Supplies","version":"1.0","type":"link"}