{"product_id":"tribioscience-tribo-mouse-ngf-elisa-kit-tbs3070","title":"Tribioscience Tribo™ Mouse NGF ELISA Kit (TBS3070)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Tribo™ Mouse NGF ELISA Kit (TBS3070)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eNerve Growth Factor (NGF) is a small secreted protein and a member of the neurotrophin family. NGF plays important roles in the growth, maintenance, and survival of certain target sympathetic and sensory neurons. NGF is expressed by inflammatory cells and affects changes that lead to increased neural responsiveness. NGF could be a pivotal inflammatory mediator in tissue injury and inflammation or pain.\u003c\/p\u003e\n\u003cp\u003eThe Tribo™ Mouse NGF ELISA Kit is designed to detect mouse NGF levels quantitatively in cell culture supernatants and different tissue samples including skin, muscle, neural, serum, and other biological samples. The plate has been coated with sheep polyclonal antibody against mouse NGF. Any NGF present in the sample or standard will be captured. A biotin-conjugated anti-murine NGF antibody is added as a detection antibody along with streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of NGF in the initial sample. The reaction is terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of NGF in the samples is calculated opposite to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDirect Assays:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003equantitative NGF in plasma and serum.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe minimum detectable dose of mouse NGF is typically less than 8.0 pg\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThis assay recognizes recombinant and natural mouse NGF. No cross-reactivity with any other cytokine was observed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 86.25%; height: 252px;\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 70.0361%; height: 19.5938px;\"\u003eReagents\u003c\/th\u003e\n\u003cth style=\"width: 29.6029%; height: 19.5938px;\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003ePrecoated 96-well Plate (8x 12 strips)\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1 Plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eStandard\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e2x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eDetection A\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eDetection B\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 100μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eSample Diluent (10x Concentrate)\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eTMB Substrate Solution\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 12mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003eWash Solution (20x Concentrate)\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e1x 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 70.0361%; height: 19.5938px;\"\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd style=\"width: 29.6029%; height: 19.5938px;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the kit at 4℃ and standard at -20℃, respectively.\u003cbr\u003eShelf life: 6 months after receipt.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 96-well plate","offer_id":40926830821434,"sku":"TBS3070","price":310.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS3070.jpg?v=1751430663","url":"https:\/\/www.msesupplies.com\/en-de\/products\/tribioscience-tribo-mouse-ngf-elisa-kit-tbs3070","provider":"MSE Supplies","version":"1.0","type":"link"}