{"product_id":"tribioscience-glucose-6-phosphate-dehydrogenase-activity-colorimetric-assay-tbs2102c","title":"Tribioscience Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay (TBS2102C)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay (TBS2102C)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGlucose 6-Phosphate dehydrogenase (G6PDH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. In the presence of NADP+ or NAD+, it oxidizes glucose-6-phosphate (G6P) to 6-Phosphogluconolactone (6PG) and NADPH or NADH. It plays a vital role in numerous biosynthetic pathways and is important for preventing oxidative damage. In humans, deficiency is an autosomal hereditary disorder that can result in the depletion of NADPH in red blood cells and increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of G6PDH has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells.\u003c\/p\u003e\n\u003cp\u003eTribioscience’s G6PDH Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting G6PDH activity in a wide variety of samples.\u003c\/p\u003e\n\u003cp\u003eIn this assay, G6PDH converts glucose-6-phosphate to 6-phosphogluconate and NAD(P)H, which in turn induces the Colorimetric Probe to generate an intense colored product at 460 nm as shown in Fig.1.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.1. Principle of the Assay\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg height=\"120\" width=\"526\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/Picture1.png\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSuitable for 96-well plate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccuracy:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eAbsorbance measurement is proportional to the G6PDH concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitive:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eCan detect as low as 0.2 mU\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTime-saving:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eJust incubate and read out style.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure G6PDH activity in a variety of samples like cells and tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.2. Typical standard curve of NADH\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/Picture2.png\" width=\"338\" height=\"245\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Tribioscience","offers":[{"title":"100 Tests","offer_id":40943072411706,"sku":"TBS2102C","price":446.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS2102C.jpg?v=1752555161","url":"https:\/\/www.msesupplies.com\/en-de\/products\/tribioscience-glucose-6-phosphate-dehydrogenase-activity-colorimetric-assay-tbs2102c","provider":"MSE Supplies","version":"1.0","type":"link"}