{"product_id":"tribioscience-fast-human-il-18-elisa-kit-tbs3239","title":"Tribioscience Fast Human IL-18 ELISA kit (TBS3239)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Fast Human IL-18 ELISA kit (TBS3239)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe Fast Human IL-18 ELISA is a solid phase ELISA designed to measure human IL-18 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg\/mL. The levels of human IL-18 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNovel Proprietary ELISA Approach\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eOnly 2 hr, not 5-6 hrs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSample and Detection combined into one step.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEasy:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eAll procedures are performed at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe low detection limit is 3.0pg\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe levels of human IL-18 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 protein.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R2= 0.9997) is provided for demonstration only in Fig.3. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-18 is typically 3.2 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-CV is 1.2%, and the Inter-CV is 10.1%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-18.\u003c\/p\u003e\n\u003cp\u003eNo-cross reactivity: Human IFN-γ; IL-18 BPa\/Fc Chimera; IL-18 Rα\/Fc Chimera; IL-18 Rβ\/Fc Chimera; Canine IL-18; Feline IL-18; Mouse IL-18; Porcine IL-18; Rat IL-18.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2018\/03\/sample-assay.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"301\" width=\"553\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/10\/TBS3239-2.jpg\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAccurate Standard Curve\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"268\" width=\"335\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/09\/standard-curve.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1240\" border=\"1px solid black\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-18\u003cbr\u003eMicroplate\u003c\/td\u003e\n\u003ctd\u003eTBS3239A\u003c\/td\u003e\n\u003ctd\u003e96-well microplate (12 strips of 8 wells) coated with a\u003cbr\u003eCapture Antibody specific for human IL-18.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch\u003cbr\u003econtaining the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman IL-18\u003cbr\u003eStandard\u003c\/td\u003e\n\u003ctd\u003eTBS3239B\u003c\/td\u003e\n\u003ctd\u003e50 µL of Recombinant human IL-18 protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost\u003cbr\u003efreezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3239C\u003c\/td\u003e\n\u003ctd\u003e2.2 ml of human IL-18 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3239D\u003c\/td\u003e\n\u003ctd\u003e120 µL of Streptavidin-HRP (100x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3239E\u003c\/td\u003e\n\u003ctd\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use an expired kit for assay.\u003cbr\u003eThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1x96-well plate","offer_id":40931200303162,"sku":"TBS3239","price":407.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS3239.jpg?v=1751691809","url":"https:\/\/www.msesupplies.com\/en-de\/products\/tribioscience-fast-human-il-18-elisa-kit-tbs3239","provider":"MSE Supplies","version":"1.0","type":"link"}