{"product_id":"tribioscience-fast-human-granzyme-a-elisa-1-step-and-1hr-tbs3207","title":"Tribioscience Fast Human Granzyme A ELISA; 1 step and 1hr (TBS3207)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience Fast Human Granzyme A ELISA; 1 step and 1hr (TBS3207)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"vc_column_container col-md-8\"\u003e\n\u003cdiv class=\"wpb_wrapper vc_column-inner\"\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e\u003cspan class=\"zw-portion zw-text-portion\" data-text-len=\"63\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eThe Fast Human Granzyme A ELISA is a solid phase ELISA designed to measure human Granzyme A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 31 to 2000pg\/mL. The levels of human Granzyme A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme A protein.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human Granzyme A is typically 5 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-CV is \u0026lt; 10%, the Inter-CV is \u0026lt; 12%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human Granzyme A.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"wpb_text_column wpb_content_element\"\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSimple Procedures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/08\/TBS3235-0.png\" alt=\"TBS3235-0\"\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3207_1.png\" width=\"517\" height=\"299\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3207_2.png\" width=\"306\" height=\"229\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"wpb_wrapper\"\u003e\n\u003ctable border=\"1px solid black\" width=\"1256\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"60\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"274\"\u003e\n\u003cstrong\u003eSTORAGE OF\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eOPENED\/\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eHuman Granzyme A Microplate\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3207A\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human Granzyme A.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eHuman Granzyme A Standard\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3207B\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e30 μL of Recombinant human Granzyme A protein (100ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3207C\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e2.1 mL of human Granzyme A antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"274\"\u003e \n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eMay be stored for up to\u003c\/p\u003e\n\u003cp\u003e3 months at 2-8 °C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3207D\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3207E\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"95\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration date.\u003c\/p\u003e\n\u003cp\u003eThe kit contains sufficient materials to run an ELISA on one 96 well plate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 96-well plate","offer_id":40928680411194,"sku":"TBS3207","price":446.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS3207.jpg?v=1751518295","url":"https:\/\/www.msesupplies.com\/en-de\/products\/tribioscience-fast-human-granzyme-a-elisa-1-step-and-1hr-tbs3207","provider":"MSE Supplies","version":"1.0","type":"link"}