{"product_id":"tribioscience-bl21-competent-cell-10x50-ul-10-9-tbs8044","title":"Tribioscience BL21 Competent Cell (10×50 uL), \u003e10^9 (TBS8044)","description":"\u003ch2 class=\"product_title entry-title show-product-nav\"\u003eTribioscience BL21 Competent Cell (10×50 uL), \u0026gt;10^9 (TBS8044)\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only. Not intended for human use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cspan data-text-len=\"63\" class=\"zw-portion zw-text-portion\"\u003eBuy Tribioscience products from MSE Supplies at the best value.\u003c\/span\u003e\u003cspan class=\"EOP-readonly EOP\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"row main-content-wrap\"\u003e\n\u003cdiv class=\"main-content col-lg-12\"\u003e\n\u003cdiv class=\"content-area\" id=\"primary\"\u003e\u003cmain class=\"site-main\" id=\"content\"\u003e\n\u003cdiv class=\"product type-product post-4091 status-publish first instock product_cat-biochemical-assays product_cat-life-sciences has-post-thumbnail taxable shipping-taxable purchasable product-type-variable product-layout-default\" id=\"product-4091\"\u003e\n\u003cdiv id=\"product-tab\" class=\"woocommerce-tabs woocommerce-tabs-1jgzs022 resp-htabs\"\u003e\n\u003cdiv class=\"resp-tabs-container\"\u003e\n\u003cdiv aria-labelledby=\"tab_item-0\" id=\"tab-description\" class=\"tab-content resp-tab-content resp-tab-content-active\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/main\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"related products\"\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"container\"\u003e\n\u003cdiv class=\"row main-content-wrap\"\u003e\n\u003cdiv class=\"main-content col-lg-12\"\u003e\n\u003cdiv class=\"content-area\" id=\"primary\"\u003e\u003cmain class=\"site-main\" id=\"content\"\u003e\n\u003cdiv class=\"product type-product post-4092 status-publish first instock product_cat-biochemical-assays has-post-thumbnail taxable shipping-taxable purchasable product-type-variable product-layout-default\" id=\"product-4092\"\u003e\n\u003cdiv id=\"product-tab\" class=\"woocommerce-tabs woocommerce-tabs-g02l8d8f resp-htabs\"\u003e\n\u003cdiv class=\"resp-tabs-container\"\u003e\n\u003cdiv aria-labelledby=\"tab_item-0\" class=\"tab-content resp-tab-content resp-tab-content-active\"\u003e\n\u003cp\u003eTribo™ E. coli BL21(DE3) Competent Cells are an E. coli strain BL21(DE3) that provides high transformation efficiencies. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under the control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces the degradation of heterologous proteins expressed in the cells.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression in E. coli.\u003c\/li\u003e\n\u003cli\u003eIPTG induction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency (cfu\/Ug)\u003cbr\u003e\u003c\/strong\u003e\u0026gt;1×10^\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue-White Screening\u003cbr\u003e\u003c\/strong\u003eNo\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003cbr\u003e\u003c\/strong\u003eNo\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStrain\u003cbr\u003e\u003c\/strong\u003eB\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGenotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eF– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the competent cells at -80°C. Shelf life: 6 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/main\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"10 x 50 uL","offer_id":40944388669498,"sku":"TBS8044-10","price":88.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TBS8044-10.jpg?v=1752665126","url":"https:\/\/www.msesupplies.com\/en-de\/products\/tribioscience-bl21-competent-cell-10x50-ul-10-9-tbs8044","provider":"MSE Supplies","version":"1.0","type":"link"}