{"product_id":"recombinant-sars-cov-2-spike-rbd-e484q-his-tag","title":"Recombinant SARS-CoV-2 Spike RBD (E484Q) (His Tag)","description":"\u003ch2\u003eRecombinant SARS-CoV-2 Spike RBD (E484Q) (His Tag)\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"height: 39.1876px;\" width=\"503\" data-mce-style=\"height: 39.1876px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\" width=\"137\" data-mce-style=\"height: 19.5938px;\"\u003eSKU #\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 388.453px;\" width=\"106\" class=\"xl66\" height=\"38\"\u003ePKSV030335\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eHEK293 Cells\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDescription\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eS protein RBD;2019-nCoV S protein RBD;SARS-CoV-2 Spike RBD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSpecies\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eSARS-CoV-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eExpression_host\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eHEK293 Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSequence\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eArg319-Phe541(E484Q)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eAccession\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eYP_009724390.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eMol_Mass\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e26.5 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eAP_Mol_Mass\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e35.1 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eTag\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eBio_Activity\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eImmobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg\/mL (100 μL\/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His(PKSV030335), the EC50 of PKSV030335 is 5.0-25.0 ng\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProperties\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003ePurity\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eEndotoxin level\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eStorage\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eGenerally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eShipping\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is provided as lyophilized powder which is shipped with ice packs.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eFormulation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003eLyophilized from sterile PBS, pH 7.4.\u003cbr\u003eNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eReconstitution\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003ePlease refer to the printed manual for detailed information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv\u003eThe spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clearfix\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv id=\"dt_tab_citation\" class=\"dt01\"\u003e\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"100μg","offer_id":40681434349626,"sku":"PKSV030335-100","price":1306.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/recombinant-sars-cov-2-n-protein-t205i-his-tag-100-g-elabscience-biotechnology-cell-assays-22333968318522.jpg?v=1752498839","url":"https:\/\/www.msesupplies.com\/en-de\/products\/recombinant-sars-cov-2-spike-rbd-e484q-his-tag","provider":"MSE Supplies","version":"1.0","type":"link"}