{"product_id":"recombinant-human-cd16a-fcgr3a-protein-176-val-his-tag-active","title":"Recombinant Human CD16a\/FCGR3A Protein (176 Val, His Tag)(Active)","description":"\u003ch2\u003eRecombinant Human CD16a\/FCGR3A Protein (176 Val, His Tag)(Active)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable style=\"height: 29.5938px;\" width=\"503\" data-mce-style=\"height: 29.5938px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 164.547px; height: 10px;\" width=\"137\" data-mce-style=\"width: 164.547px; height: 10px;\"\u003eSKU #\u003c\/td\u003e\n\u003ctd height=\"38\" class=\"xl65\" width=\"106\" style=\"height: 10px; width: 367.453px;\"\u003ePKSH030286\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 164.547px; height: 19.5938px;\" data-mce-style=\"width: 164.547px; height: 19.5938px;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"width: 367.453px; height: 19.5938px;\" data-mce-style=\"width: 367.453px; height: 19.5938px;\"\u003e\u003cspan\u003eHEK293 Cells\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDescription    \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"dt01\"\u003e\n\u003cdiv id=\"dt_tab1\" class=\"dt01\"\u003e\n\u003ctable style=\"width: 100%; height: 176.344px;\" class=\"dtl_table\" data-mce-style=\"width: 100%; height: 176.344px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\" width=\"170\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eCD16, CD16A, CD16a, CD16a Antigen, FCG3, FCGR3, FCGR3A, Fc-Gamma RIII, Fc-Gamma RIII-Alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc Receptor III-2, Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eSpecies\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\" width=\"170\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eHEK293 Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eSequence\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eMet 1-Gln 208\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eAccession\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eAAH17865.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eCalculated Molecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003e23.3 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eObserved Molecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003e48 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eTag\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 43.2347%;\"\u003eBio-activity\u003c\/td\u003e\n\u003ctd style=\"width: 56.3963%;\"\u003e1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80nM. 2. Immobilized human CD16a-His(176 Val) at 10ug\/ml(100ul\/well ) can bind human IgG1 with a linear range of 0.00128-0.8 μg\/ml.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n  \u003c\/div\u003e\n\u003cdiv id=\"dt_tab2\" class=\"dt01 detail_section\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eProperties\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 305.5px;\" class=\"dtl_table\" data-mce-style=\"width: 100%; height: 305.5px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 19.5938px;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 19.5938px;\"\u003e\u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\" data-mce-style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 39.1875px;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 39.1875px;\"\u003e\u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 78.375px;\" data-mce-style=\"height: 78.375px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 78.375px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 78.375px;\"\u003eGenerally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\" data-mce-style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 39.1875px;\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 39.1875px;\"\u003eThis product is provided as lyophilized powder which is shipped with ice packs.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 97.9688px;\" data-mce-style=\"height: 97.9688px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 97.9688px;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 97.9688px;\"\u003eLyophilized from sterile PBS, pH 7.4\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 50.7812px;\" data-mce-style=\"height: 50.7812px;\"\u003e\n\u003ctd style=\"width: 25.7353%; height: 20.7656px;\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd style=\"width: 73.8957%; height: 20.7656px;\"\u003ePlease refer to the printed manual for detailed information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"dt_tab3\" class=\"dt01\"\u003e\n\u003ch3\u003e\n\u003cspan class=\"title\"\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cspan class=\"title\"\u003eBackground\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab3\"\u003eThe Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes\/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a \/ FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a \/ FCGR3A also has a broader function. CD16a \/ FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a \/ FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a \/ FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"100μg","offer_id":40692818575418,"sku":"PKSH030286-100","price":864.95,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":40692818608186,"sku":"PKSH030286-20","price":6941.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/recombinant-human-cd16a-fcgr3a-protein-176-val-his-tag-active-100-g-elabscience-biotechnology-cell-assays-22373401264186.jpg?v=1752498364","url":"https:\/\/www.msesupplies.com\/en-de\/products\/recombinant-human-cd16a-fcgr3a-protein-176-val-his-tag-active","provider":"MSE Supplies","version":"1.0","type":"link"}