{"product_id":"mse-pro-rat-smad3-mothers-against-decapentaplegic-homolog-3-elisa-kit","title":"MSE PRO Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit","description":"\u003ch2\u003eMSE PRO™ Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit\u003c\/h2\u003e\n\u003ch3\u003eSpecifications:\u003c\/h3\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name\u003c\/td\u003e\n\u003ctd\u003eRat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003eJV15-2; MADH3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e0.079 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd\u003e0.16-10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003etissue homogenates, cell lysates and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eSignal transduction; Tumor immunity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eUniprot ID\u003c\/td\u003e\n\u003ctd\u003eP84025\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Smad3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Smad3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Smad3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Smad3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"MSE Supplies","offers":[{"title":"96T","offer_id":41020474032186,"sku":"EK0064478-96T","price":264.95,"currency_code":"USD","in_stock":true},{"title":"48T","offer_id":41020474064954,"sku":"EK0064478-48T","price":375.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/EK0064478.jpg?v=1760174559","url":"https:\/\/www.msesupplies.com\/en-de\/products\/mse-pro-rat-smad3-mothers-against-decapentaplegic-homolog-3-elisa-kit","provider":"MSE Supplies","version":"1.0","type":"link"}