{"product_id":"himedia-l-glutamine-100g-10g","title":"HIMEDIA L-Glutamine","description":"\u003ch2\u003eHIMEDIA L-Glutamine 100G, 10G\u003c\/h2\u003e\n\u003ch3 style=\"text-align: left;\"\u003eProduct Description:\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 146.14\u003cbr\u003e\u003cstrong\u003eMolecular Formula:\u003c\/strong\u003e H2NCOCH2CH2CH(NH2)CO2H\u003cbr\u003e\u003cstrong\u003eCAS No:\u003c\/strong\u003e 56-85-9\u003cbr\u003e\u003cstrong\u003eSynonym:\u003c\/strong\u003e (S)-2, 5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide \u003c\/p\u003e\n\u003cp\u003eL-Glutamine is polar, hydrophilic, essential α-amino acid coded by codons CAA and CAG. It is chemically acidic in nature. It is an aminated analog of L-Glutamate. \u003c\/p\u003e\n\u003cp\u003eL-Glutamine and L-Glutamate are inter-convertible amino acids. L-Glutamine is synthesized from ammonium and glutamate with the help of enzyme glutamine synthetase. This reaction consumes energy in form of ATP. On the other hand, catabolism of L-Glutamine leads to formation of glutamate and ammonium. This reaction is catalyzed by mitochondrial enzyme glutaminase. Ammonium ions generated in this reaction are converted to urea under in vivo conditions. However, under in vitro environment ammonium is not converted to urea. It accumulates in extracellular environment and can be toxic for cells. When L-Glutamine is present as an amino acid residue in proteins or peptides it is stable; however when it is present in its free form, it is unstable. \u003c\/p\u003e\n\u003cp\u003eOwing to instability of L-Glutamine in liquid media and accumulation of toxic ammonia in culture system, concentration of L-Glutamine should be optimized for every type of cell culture process. \u003c\/p\u003e\n\u003cp\u003eIn cell culture systems, L-Glutamine serves as a crucial component of culture media. It is used extensively in wide range of media including classical media, serum-free media, insect cell culture media and media for hybridoma culturing. It plays many important roles in cell culture. Some of them are mentioned below: \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnergy source\u003c\/strong\u003e\u003cbr\u003eL-Glutamine serves as an alternative source of energy for rapidly dividing cells and cells that use glucose inefficiently. L-Glutamine is readily available amino acid for energy production when carbohydrate energy source is not available or deprived in culture medium.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReservoir of nitrogen for synthesis of proteins, nucleic acid and other nitrogenous compounds:\u003c\/strong\u003e\u003cbr\u003eL-Glutamine is converted to ammonium and pyroglutamate inside the cells. Cells utilize these ammonium ions for formation of amine of glutamate or amide of glutamine. These two amino acid derivatives serve as primary reservoirs of nitrogen for synthesis of\u003cbr\u003eproteins, vitamins, nucleic acids and other nitrogenous compounds such as NAD, NADH. \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e Precursor of glutamate:\u003c\/strong\u003e\u003cbr\u003eL-Glutamic acid generated from catabolism of LGlutamine serves as a precursor for synthesis of alpha amino acids.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth-limiting amino acid:\u003c\/strong\u003e\u003cbr\u003eL-Glutamine acts as a growth-limiting amino acid in culture media and hence is required at a 5- to 20-fold greater concentration than other amino acids. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eDirections: \u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003ePreparation instructions:\u003c\/strong\u003e\u003cbr\u003eL-Glutamine is soluble in water. It is insoluble in alcohol and ether. L-Glutamine solutions cannot be autoclaved as it is temperature-sensitive. They are sterilized by filtering through a sterile membrane of porosity 0.22 micron or less. \u003c\/p\u003e\n\u003cp\u003eRecommended concentration of L-Glutamine ranges from 0.5 to 10mM depending on type of medium. Commonly used concentrations of L-Glutamine in some of the animal\u003cbr\u003ecell culture media are mentioned in the table:\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; border: none; width: 87.5%; height: 235px;\" cellpadding=\"0\" cellspacing=\"0\" border=\"1\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e\u003cb\u003eName of the Medium\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e\u003cb\u003eConcentration of L-Glutamine (mM) \u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eAME's Medium\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e0.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eBME\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eDMEM\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eMEM\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eLeibovitz's L-15\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2.05\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eDMEM-F12\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2.5\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eMcCoy's 5A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eMCDB\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e4-10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eIMDM\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eGMEM\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 47.4061%; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003eRPMI\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 52.5939%; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 19px;\" valign=\"top\" width=\"301\"\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal; text-align: center;\" class=\"MsoNormal\"\u003e2.054\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eInsect cells require higher concentration of amino acids as compared animal cells. Hence L-Glutamine concentration for insect cell culture media ranges from 4 to 12.3mM. To avoid decomposition of L-Glutamine during storage sterile stock solutions of L-Glutamine should be added in the medium just prior to use\u003c\/p\u003e","brand":"HIMEDIA","offers":[{"title":"100G","offer_id":41016261410874,"sku":"TC243-100G","price":300.95,"currency_code":"USD","in_stock":true},{"title":"10G","offer_id":41016261443642,"sku":"TC243-10G","price":52.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TC243-100G.jpg?v=1758613263","url":"https:\/\/www.msesupplies.com\/en-de\/products\/himedia-l-glutamine-100g-10g","provider":"MSE Supplies","version":"1.0","type":"link"}