{"product_id":"glutathione-peroxidase-gsh-px-activity-assay-kit-1","title":"Glutathione Peroxidase (GSH-Px) Activity Assay Kit","description":"\u003ch2\u003eGlutathione Peroxidase (GSH-Px) Activity Assay Kit\u003c\/h2\u003e\n\u003ctable style=\"height: 48px; width: 554px;\" width=\"503\" data-mce-style=\"height: 48px; width: 554px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 149.1px;\" data-mce-style=\"height: 10px; width: 149.1px;\"\u003e\u003cstrong\u003eSKU #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 10px; width: 391.3px;\" data-mce-style=\"height: 10px; width: 391.3px;\"\u003eE-BC-K096-S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 149.1px;\" data-mce-style=\"height: 19px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 391.3px;\" data-mce-style=\"height: 19px; width: 391.3px;\"\u003eSpectrophotometer(412 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 149.1px;\" data-mce-style=\"height: 19px; width: 149.1px;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 391.3px; height: 19px;\" data-mce-style=\"width: 391.3px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 298px; width: 555px;\" class=\"dtl_table2\" data-mce-style=\"font-size: 0.875rem; height: 298px; width: 555px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 22px;\" data-mce-style=\"width: 158.788px; height: 22px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 22px;\" data-mce-style=\"width: 367.612px; height: 22px;\"\u003e\u003cspan\u003eGSH-PX\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.788px;\" data-mce-style=\"height: 19px; width: 158.788px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003e\u003cspan\u003eSerum, plasma, cells, cell culture supernatant, tissue\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 10px;\" data-mce-style=\"width: 158.788px; height: 10px;\"\u003e\u003cspan\u003eSensitivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 10px;\" data-mce-style=\"width: 367.612px; height: 10px;\"\u003e\u003cspan\u003e12.65 U\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eDetection Range\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px;\" data-mce-style=\"width: 367.612px;\"\u003e12.65-387 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eDetection Method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003e\u003cspan\u003eColorimetric method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eAssay type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003e\u003cspan\u003eEnzyme Activity\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px;\" data-mce-style=\"width: 367.612px;\"\u003e70 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003ePrecision\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003eAverage inter-assay CV: 9.300% | Average intra-assay CV: 4.900%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eOther instruments required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px;\" data-mce-style=\"width: 367.612px;\"\u003eMicropipettor, Incubator, Vortex mixer, Centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.788px; height: 19px;\" data-mce-style=\"width: 158.788px; height: 19px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003e\u003cspan\u003eNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"height: 10px; width: 158.788px;\" data-mce-style=\"height: 10px; width: 158.788px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 10px;\" data-mce-style=\"width: 367.612px; height: 10px;\"\u003e\u003cspan\u003e2-8℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.788px;\" data-mce-style=\"height: 19px; width: 158.788px;\"\u003eValid period\u003c\/td\u003e\n\u003ctd style=\"width: 367.612px; height: 19px;\" data-mce-style=\"width: 367.612px; height: 19px;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eImages\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eN V Mohamad et al investigate the therapeutic effect of annatto tocotrienol for postmenopausal bone loss. Glutathione peroxidase (GSH-Px) activity of rat serum was determined using GSH-Px activity assay kit (E-BC-K096-S).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"title\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/image\/product\/biochemical_kits\/citation\/E-BC-K096-S-CI.png\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"336\" height=\"206\" data-mce-src=\"https:\/\/file.elabscience.com\/image\/product\/biochemical_kits\/citation\/E-BC-K096-S-CI.png\" data-mce-style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"title\"\u003e\u003cspan\u003eA significant decline of GSH-Px activity due to ovariectomy (OVX) was observed (P=0.039). Treatment with annatto tocotrienol (ANTT) and Raloxifene (RAL) elevated GSH-Px activity.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"col-xs-12 col-sm-7\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eDilution of Sample\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eThe\u003cspan\u003e \u003c\/span\u003eoptimal\u003cspan\u003e \u003c\/span\u003esampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio\u003cspan\u003e \u003c\/span\u003eis 20%~60% (\u003cspan\u003ethe \u003c\/span\u003eoptimal\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003einhibition ratio is the range of 45%~55%.\u003c\/span\u003e) before formal experiment.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-mce-style=\"font-weight: 400;\"\u003eNote: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eDetection Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGlutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/image\/product\/biochemical_kits\/principle\/E-BC-K096-S-principle.png\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"328\" height=\"73\" data-mce-src=\"https:\/\/file.elabscience.com\/image\/product\/biochemical_kits\/principle\/E-BC-K096-S-principle.png\" data-mce-style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong\u003eKit Components \u0026amp; Storage\u003c\/strong\u003e\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"639\" style=\"height: 321px; width: 550px;\" data-mce-style=\"height: 321px; width: 550px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px; text-align: left;\" data-mce-style=\"width: 75.2625px; height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px; text-align: left;\" data-mce-style=\"width: 110.375px; height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px; text-align: left;\" data-mce-style=\"width: 108.363px; height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\n\u003cstrong\u003eSize 1 \u003c\/strong\u003e\u003cstrong\u003e(50 assays)\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; text-align: left; height: 19px;\" data-mce-style=\"width: 100.338px; text-align: left; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSize 2 (100 assays)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px; text-align: left;\" data-mce-style=\"width: 126.463px; height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 1\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\" data-mce-fragment=\"1\"\u003eStock Solution\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 mL × 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e2 mL × 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 2\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\" data-mce-fragment=\"1\"\u003eAcid Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\" data-mce-fragment=\"1\"\u003e60 mL× 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e60 mL× 4 vials\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 3\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\" data-mce-fragment=\"1\"\u003ePhosphate\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\" data-mce-fragment=\"1\"\u003ePowder×1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003ePowder ×2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\" data-mce-fragment=\"1\"\u003eReagent 4\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\" data-mce-fragment=\"1\"\u003eDTNB Solution\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\" data-mce-fragment=\"1\"\u003e15 mL× 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e30 mL× 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃, 12 months,\u003cbr\u003eshading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\"\u003eReagent 5\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\"\u003eSalt Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\"\u003ePowder × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003ePowder × 4 vials\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\"\u003e2-8℃, 12 months,\u003cbr\u003eshading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px; text-align: left;\" data-mce-style=\"height: 19px; text-align: left;\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\"\u003eReagent 6\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\"\u003eGSH Standard\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\"\u003e3.07 mg × 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e3.07 mg × 2 vials\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 75.2625px; height: 19px;\" data-mce-style=\"width: 75.2625px; height: 19px;\"\u003eReagent 7\u003c\/td\u003e\n\u003ctd style=\"width: 110.375px; height: 19px;\" data-mce-style=\"width: 110.375px; height: 19px;\"\u003eGSH Standard Stock Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 108.363px; height: 19px;\" data-mce-style=\"width: 108.363px; height: 19px;\"\u003e3 mL× 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 100.338px; height: 19px;\" data-mce-style=\"width: 100.338px; height: 19px;\"\u003e6 mL × 1 vial\u003c\/td\u003e\n\u003ctd style=\"width: 126.463px; height: 19px;\" data-mce-style=\"width: 126.463px; height: 19px;\"\u003e2-8℃, 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eNote: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eParameter:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 48px; width: 100%;\" width=\"100%\" data-mce-style=\"font-size: 0.875rem; height: 48px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eMean (U)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e24.60\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e135.70\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e264.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 10px;\" data-mce-style=\"width: 28.5584%; height: 10px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 10px;\" data-mce-style=\"width: 21.7611%; height: 10px;\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 10px;\" data-mce-style=\"width: 23.7683%; height: 10px;\"\u003e4.8\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 10px;\" data-mce-style=\"width: 24.8175%; height: 10px;\"\u003e4.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThree human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"font-size: 0.875rem; height: 53px; width: 100%;\" data-mce-style=\"font-size: 0.875rem; height: 53px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003eParameters\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 15px;\" data-mce-style=\"width: 28.5584%; height: 15px;\"\u003e\u003cstrong\u003eMean (U)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 15px;\" data-mce-style=\"width: 21.7611%; height: 15px;\"\u003e24.60\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 15px;\" data-mce-style=\"width: 23.7683%; height: 15px;\"\u003e135.70\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 15px;\" data-mce-style=\"width: 24.8175%; height: 15px;\"\u003e264.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 28.5584%; height: 19px;\" data-mce-style=\"width: 28.5584%; height: 19px;\"\u003e\u003cstrong\u003e%CV \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.7611%; height: 19px;\" data-mce-style=\"width: 21.7611%; height: 19px;\"\u003e9.8\u003c\/td\u003e\n\u003ctd style=\"width: 23.7683%; height: 19px;\" data-mce-style=\"width: 23.7683%; height: 19px;\"\u003e9.4\u003c\/td\u003e\n\u003ctd style=\"width: 24.8175%; height: 19px;\" data-mce-style=\"width: 24.8175%; height: 19px;\"\u003e8.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTake three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 73px; width: 100%;\" width=\"100%\" data-mce-style=\"font-size: 0.875rem; height: 73px; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 20px;\" data-mce-style=\"width: 32.573%; height: 20px;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 20px;\" data-mce-style=\"width: 21.0311%; height: 20px;\"\u003e\u003cstrong\u003eSample 1\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 20px;\" data-mce-style=\"width: 20.6662%; height: 20px;\"\u003e\u003cstrong\u003eSample 2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 20px;\" data-mce-style=\"width: 24.635%; height: 20px;\"\u003e\u003cstrong\u003eSample 3\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 15px;\" data-mce-style=\"height: 15px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 15px;\" data-mce-style=\"width: 32.573%; height: 15px;\"\u003e\u003cstrong\u003eExpected Conc. (U)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 15px;\" data-mce-style=\"width: 21.0311%; height: 15px;\"\u003e75.5\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 15px;\" data-mce-style=\"width: 20.6662%; height: 15px;\"\u003e184.2\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 15px;\" data-mce-style=\"width: 24.635%; height: 15px;\"\u003e244.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 19px;\" data-mce-style=\"width: 32.573%; height: 19px;\"\u003e\u003cstrong\u003eObserved Conc. (U)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 19px;\" data-mce-style=\"width: 21.0311%; height: 19px;\"\u003e77.0\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 19px;\" data-mce-style=\"width: 20.6662%; height: 19px;\"\u003e198.9\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 19px;\" data-mce-style=\"width: 24.635%; height: 19px;\"\u003e256.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 32.573%; height: 19px;\" data-mce-style=\"width: 32.573%; height: 19px;\"\u003e\u003cstrong\u003eRecovery rate (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0311%; height: 19px;\" data-mce-style=\"width: 21.0311%; height: 19px;\"\u003e102\u003c\/td\u003e\n\u003ctd style=\"width: 20.6662%; height: 19px;\" data-mce-style=\"width: 20.6662%; height: 19px;\"\u003e108\u003c\/td\u003e\n\u003ctd style=\"width: 24.635%; height: 19px;\" data-mce-style=\"width: 24.635%; height: 19px;\"\u003e105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe analytical sensitivity of the assay is 12.65 U. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAs the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:\u003c\/p\u003e\n\u003ctable style=\"height: 77px; width: 546px;\" width=\"662\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 39px; width: 143.475px;\" data-mce-style=\"height: 39px; width: 139.238px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eConcentration (μmol\/L)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 53.2875px;\" data-mce-style=\"height: 39px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 53.2875px;\" data-mce-style=\"height: 39px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e10\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 53.2875px;\" data-mce-style=\"height: 39px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e20\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 53.2875px;\" data-mce-style=\"height: 39px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e40\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 53.2875px;\" data-mce-style=\"height: 39px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e60\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 47.6px; height: 39px;\" data-mce-style=\"width: 44.5875px; height: 39px;\"\u003e\u003cstrong\u003e80\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 43.6875px; height: 39px;\"\u003e\u003cstrong\u003e100\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 143.475px;\" data-mce-style=\"height: 19px; width: 139.238px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAverage OD\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.072\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.125\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.187\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.307\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.424\u003c\/td\u003e\n\u003ctd style=\"width: 47.6px; height: 19px;\" data-mce-style=\"width: 44.5875px; height: 19px;\"\u003e0.536\u003c\/td\u003e\n\u003ctd style=\"width: 43.6875px; height: 19px;\"\u003e0.660\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"height: 19px; width: 143.475px;\" data-mce-style=\"height: 19px; width: 139.238px;\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003eAbsoluted OD\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.053\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.115\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.235\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 53.2875px;\" data-mce-style=\"height: 19px; width: 53.0625px;\" data-mce-fragment=\"1\"\u003e0.352\u003c\/td\u003e\n\u003ctd style=\"width: 47.6px; height: 19px;\" data-mce-style=\"width: 44.5875px; height: 19px;\"\u003e0.464\u003c\/td\u003e\n\u003ctd style=\"width: 43.6875px; height: 19px;\"\u003e0.588\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"100 Assays","offer_id":40374905962554,"sku":"E-BC-K096-S-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40374905995322,"sku":"E-BC-K096-S-50","price":202.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-BC-K096-S.jpg?v=1712940371","url":"https:\/\/www.msesupplies.com\/en-de\/products\/glutathione-peroxidase-gsh-px-activity-assay-kit-1","provider":"MSE Supplies","version":"1.0","type":"link"}