{"product_id":"anti-human-cd3-apc-cd4-fitc-cd8a-pe-cocktail","title":"Anti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail","description":"\u003ch2\u003eAnti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"503\" style=\"height: 78.4px;\" data-mce-style=\"height: 78.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" width=\"137\" data-mce-style=\"height: 19.6px;\"\u003eSKU #\u003c\/td\u003e\n\u003ctd height=\"39\" class=\"xl65\" width=\"63\" style=\"height: 19.5938px; width: 333.359px;\" data-mce-style=\"height: 19.5938px; width: 333.359px;\"\u003eE-AB-FC0008\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003eFCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 200.73%;\" class=\"dtl_table2\" data-mce-style=\"width: 200.73%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eForm\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eClone No.\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003e\u003ca href=\"https:\/\/www.elabscience.com\/search-category=Flow%20Cytometry%20Antibodies\u0026amp;keywords=OKT-3,RPA-T4,OKT-8.html\" target=\"_blank\"\u003e\u003cu\u003eOKT-3,RPA-T4,OKT-8\u003c\/u\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eReactivity\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003e\u003ca class=\"text-primary\" href=\"https:\/\/www.elabscience.com\/List-detail-5967.html\" target=\"_blank\"\u003e\u003cu\u003eFCM\u003c\/u\u003e\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003ePhosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eRecommended Use\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eFor whole blood samples, add 5 μL Anti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003edissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eBiological ice pack at 4℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eStability \u0026amp; Storage\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eKeep as concentrated solution.\u003cbr\u003eStore at 2~8℃ and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003eCentrifuge before opening to ensure complete recovery of vial contents.\u003cbr\u003eThis product is guaranteed up to one year from purchase.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003eConjugation\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eAPC,FITC,PE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.1635%;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 84.6536%;\"\u003eAPC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660\/20 nm bandpass filter). FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525\/40 nm bandpass filter). PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585\/42 nm bandpass filter).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"2\" style=\"width: 99.8172%;\" data-mce-style=\"width: 99.8172%;\"\u003e\n\u003cp class=\"conju-pic\"\u003e\u003cimg height=\"513\" width=\"685\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Capture_f02937eb-c25c-42f0-840c-a26ca0329c10.jpg?v=1725950025\" alt=\"\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Capture_f02937eb-c25c-42f0-840c-a26ca0329c10.jpg?v=1725950025\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFluorophore\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConjugation: \u003c\/strong\u003eAPC,FITC,PE\u003c\/p\u003e\n\u003cp\u003eAPC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660\/20 nm bandpass filter). FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525\/40 nm bandpass filter). PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585\/42 nm bandpass filter).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended usage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor whole blood samples, add 5 μL Anti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail to 100 μL anticoagulanttreated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.\u003cbr\u003eFor other samples, 1×10\u003csup\u003e6\u003c\/sup\u003e dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-APC\/CD4-FITC\/CD8a-PE Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.\u003c\/p\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"20 Tests","offer_id":40633837322298,"sku":"E-AB-FC0008-20","price":113.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40633837355066,"sku":"E-AB-FC0008-100","price":278.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests (2x100)","offer_id":40633837387834,"sku":"E-AB-FC0008-200","price":472.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/apc-anti-human-cd193-ccr3-antibody-5e8-20-tests-elabscience-biotechnology-biomarkers-22096977002554.jpg?v=1752501060","url":"https:\/\/www.msesupplies.com\/en-de\/products\/anti-human-cd3-apc-cd4-fitc-cd8a-pe-cocktail","provider":"MSE Supplies","version":"1.0","type":"link"}