{"product_id":"10x-rbc-lysis-fixation-solution","title":"10× RBC Lysis\/Fixation Solution","description":"\u003ch2\u003e10× RBC Lysis\/Fixation Solution\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"left\" class=\"MsoNormal\"\u003e\u003cspan\u003eElabscience\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e® \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e10×RBC Lysis\/Fixation Solution is designed for FCM sample processing of human whole blood. The whole blood is treated with anticoagulant and incubated with fluorescent antibody, the whole blood is added with the RBC Lysis\/Fixation Solution to lyse excess red blood cells for FCM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\" class=\"MsoNormal\"\u003e\u003cspan\u003eWhen added to the whole blood, antibodies bind to specific surface antigen on leukocyte. After treatment with 1× RBC Lysis\/Fixation Working solution, the erythrocytes are lysed under mild hypotonic conditions, keeping intact viability of white blood cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cb\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10× RBC Lysis\/Fixation Solution is concentrated, dilute with ddH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO to 1× working solution before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFor example: take 1 mL 10× RBC Lysis\/Fixation Solution [E-CK-A106], add to 9 mL ddH\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003eO.The mixture is 1×RBC Lysis\/Fixation Working Solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cb\u003eSample was treated by \u003c\/b\u003e\u003cb\u003e10\u003c\/b\u003e\u003cb\u003e×\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003e\u003cb\u003eRBC Lysis\/Fixation Solution[\u003c\/b\u003e\u003cb\u003eE-CK-A106]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\" class=\"MsoNormal\"\u003e\u003cspan\u003eAdd 1× lysis solution to the sample for red blood cell lysis. The supernatant was discarded after centrifugation. Cells were analyzed with flow cytometry. Cell debris and platelets can be excluded by a diagonal gate (Left) in the FSC-H vs FSC-A diagram. Lymphocytes, monocytes and granulocytes are separated clearly (Right).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\"\u003e\u003cb\u003e\u003cspan\u003eExperimental Procedure\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003eAdd 100 μL of whole blood to a FCM tube.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAdd appropriate volume of FCM antibody\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eto the tube (refer to the antibody manual).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eVortex gently and incubate at 4°C in the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003edark for 30 min.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAdd 2 mL of 1×RBC Lysis\/Fixation\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eWorking Solution.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eVortex gently and incubate at RT in the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003edark for 10 min.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eCentrifuge at 500 g for 5 min\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAdd 2 mL PBS to resuspend the cells.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCentrifuge at 500 g for 5 min. Discard \u003c\/span\u003e\u003cspan\u003ethe supernatant.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAdd 0.5 mL Cell Staining Buffer to \u003c\/span\u003e\u003cspan\u003eresuspend the cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAnalyze the cells immediately with \u003c\/span\u003e\u003cspan\u003eproper machine settings.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan new=\"\" times=\"\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at \u003c\/span\u003e\u003cspan\u003e2~8°C. Avoid of freezing. Valid for 12 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp class=\"MsoListParagraph\" align=\"left\" style=\"font-size: 0.875rem; display: inline !important;\"\u003eUse EDTA as the anticoagulant instead of heparin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eRed blood cells may show in samples with nucleated red blood cells (such as pediatric samples) for the solution lyzes only enucleated red blood cells.\u003c\/li\u003e\n\u003cli\u003eIF the FCM antibodies react with serum immunoglobulins, blood samples should be washed with 1×PBS or physiological saline prior to staining and lysing.\u003c\/li\u003e\n\u003cli\u003eThis solution contains fixative which may damage fluorescence of tandem dyes such as APC\/cyanine7.\u003c\/li\u003e\n\u003cli\u003eThis reagent is for research use only and not for use in diagnostic procedures. For your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40448731021370,"sku":"E-CK-A106-500","price":184.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40448731086906,"sku":"E-CK-A106-100","price":122.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40448731152442,"sku":"E-CK-A106-50","price":99.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A106.jpg?v=1714378124","url":"https:\/\/www.msesupplies.com\/en-de\/products\/10x-rbc-lysis-fixation-solution","provider":"MSE Supplies","version":"1.0","type":"link"}