{"title":"ELISA Kits","description":"\u003cp\u003eShop high-quality ELISA kits from MSE Supplies, designed for accurate and sensitive detection of proteins, antibodies, and other biomolecules in research and diagnostic applications. Our collection includes ready-to-use kits for quantitative and qualitative analysis, ensuring reproducible results across a wide range of sample types. Ideal for immunology, proteomics, and life science studies, these ELISA kits offer exceptional specificity, reliability, and ease of use. Choose from various formats and targets to streamline your workflow and achieve precise measurements. Order now for trusted ELISA solutions that support cutting-edge research.\u003c\/p\u003e","products":[{"product_id":"vb6-vitamin-b6-elisa-kit","title":"VB6(Vitamin B6) ELISA Kit","description":"\u003ch2\u003eVB6(Vitamin B6) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 263px;\" width=\"210\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132.219px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 114.781px;\"\u003e 1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132.219px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 114.781px;\"\u003e 0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 269px;\" class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eAssay type\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eAssay time\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eDetection range\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\"\u003e\n\u003ctd style=\"height: 39px; width: 158.594px;\"\u003eSample type \u0026amp; Sample volume\u003c\/td\u003e\n\u003ctd style=\"height: 39px; width: 375.406px;\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\"\u003e\n\u003ctd style=\"height: 58px; width: 158.594px;\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 375.406px;\"\u003eThis kit recognizes VB6 in samples. No significant cross-reactivity or interference between VB6 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 158.594px;\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 375.406px;\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58px;\"\u003e\n\u003ctd style=\"height: 58px; width: 158.594px;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"height: 58px; width: 375.406px;\"\u003eThis ELISA kit applies to the in vitro quantitative determination of VB6 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with VB6. During the reaction, VB6 in the sample or standard competes with a fixed amount of VB6 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VB6. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB6 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\" width=\"277\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"141\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"208\" data-mce-style=\"text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e-20℃ (Protect from light), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e5 pieces\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level VB6 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level VB6 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e4.75\u003c\/td\u003e\n\u003ctd\u003e14.63\u003c\/td\u003e\n\u003ctd\u003e46.67\u003c\/td\u003e\n\u003ctd\u003e4.50\u003c\/td\u003e\n\u003ctd\u003e13.37\u003c\/td\u003e\n\u003ctd\u003e51.21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e0.25\u003c\/td\u003e\n\u003ctd\u003e0.63\u003c\/td\u003e\n\u003ctd\u003e2.41\u003c\/td\u003e\n\u003ctd\u003e0.30\u003c\/td\u003e\n\u003ctd\u003e0.68\u003c\/td\u003e\n\u003ctd\u003e2.02\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e5.26\u003c\/td\u003e\n\u003ctd\u003e4.31\u003c\/td\u003e\n\u003ctd\u003e5.16\u003c\/td\u003e\n\u003ctd\u003e6.67\u003c\/td\u003e\n\u003ctd\u003e5.09\u003c\/td\u003e\n\u003ctd\u003e3.95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of VB6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e91-106\u003c\/td\u003e\n\u003ctd\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e88-99\u003c\/td\u003e\n\u003ctd\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e90-103\u003c\/td\u003e\n\u003ctd\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003eVB6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eSignal Transduction, Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40303001305146,"sku":"E-EL-0008","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/VB6_VitaminB6_ELISAKit2.jpg?v=1710273042"},{"product_id":"fa-vb9-folic-acid-vitamin-b9-elisa-kit","title":"FA\/VB9(Folic Acid\/Vitamin B9) ELISA Kit","description":"\u003ch2\u003eFA\/VB9(Folic Acid\/Vitamin B9) ELISA Kit\u003c\/h2\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e 0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003esize\u003c\/td\u003e\n\u003ctd\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eThis kit recognizes Universal FA\/VB9 in samples.No significant cross-reactivity or interference between Universal FA\/VB9 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003ePrecision\u003c\/td\u003e\n\u003ctd\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eRecovery\u003c\/td\u003e\n\u003ctd\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd\u003eThis ELISA kit applies to the in vitro quantitative determination of FA\/VB9 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal FA\/VB9. During the reaction, Universal FA\/VB9 in the sample or standard competes with a fixed amount of Universal FA\/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FA\/VB9. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal FA\/VB9 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level FA\/VB9 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level FA\/VB9 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 74.6023px;\"\u003e5.4\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e8.46\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e35.72\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e4.86\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e8.06\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.6335px;\"\u003e38.99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 74.6023px;\"\u003e0.37\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e0.48\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e1.96\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e0.28\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e0.36\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.6335px;\"\u003e2.09\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 74.6023px;\"\u003e6.85\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e5.67\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e5.49\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 70.5682px;\"\u003e5.76\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.5767px;\"\u003e4.47\u003c\/td\u003e\n\u003ctd data-mce-style=\"width: 71.6335px;\"\u003e5.36\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of FA\/VB9 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e85-100\u003c\/td\u003e\n\u003ctd\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e90-101\u003c\/td\u003e\n\u003ctd\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e101-117\u003c\/td\u003e\n\u003ctd\u003e108\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eCell Biology, Metabolism, Immunology, Developmental Biology, Biochemicals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40345759055930,"sku":"E-EL-0009","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ufT0eH.jpg?v=1712133415"},{"product_id":"vd-vitamin-d-elisa-kit","title":"VD(Vitamin D) ELISA Kit","description":"\u003ch2\u003eVD(Vitamin D) ELISA Kit\u003c\/h2\u003e\n\u003ctable width=\"210\" style=\"width: 252px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e6.25-400 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e3.75 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.25-400 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.75 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal VD in samples.No significant cross-reactivity or interference between Universal VD and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of VD concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD. During the reaction, Universal VD in the sample or standard competes with a fixed amount of Universal VD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level VD were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level VD were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\"\u003e\u003cb\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e21.62\u003c\/td\u003e\n\u003ctd\u003e53.66\u003c\/td\u003e\n\u003ctd\u003e157.91\u003c\/td\u003e\n\u003ctd\u003e21.41\u003c\/td\u003e\n\u003ctd\u003e57.3\u003c\/td\u003e\n\u003ctd\u003e152.81\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e1.28\u003c\/td\u003e\n\u003ctd\u003e2.7\u003c\/td\u003e\n\u003ctd\u003e5.98\u003c\/td\u003e\n\u003ctd\u003e1.47\u003c\/td\u003e\n\u003ctd\u003e2.35\u003c\/td\u003e\n\u003ctd\u003e8.34\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e5.92\u003c\/td\u003e\n\u003ctd\u003e5.03\u003c\/td\u003e\n\u003ctd\u003e3.79\u003c\/td\u003e\n\u003ctd\u003e6.87\u003c\/td\u003e\n\u003ctd\u003e4.1\u003c\/td\u003e\n\u003ctd\u003e5.46\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of VD spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e96-112\u003c\/td\u003e\n\u003ctd\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e88-99\u003c\/td\u003e\n\u003ctd\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd\u003e85-97\u003c\/td\u003e\n\u003ctd\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003e Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353357398074,"sku":"E-EL-0012","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/vd-vitamin-d-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21857351565370.jpg?v=1752497803"},{"product_id":"vd3-vitamin-d3-elisa-kit","title":"VD3(Vitamin D3) ELISA Kit","description":"\u003ch2\u003eVD3(Vitamin D3) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 252px;\" width=\"210\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal VD3 in samples.No significant cross-reactivity or interference between Universal VD3 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of VD3 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD3. During the reaction, Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level VD3 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level VD3 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.75\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e12.74\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e45.67\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.34\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e12.36\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e44.41\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.29\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.63\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.42\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.67\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e2.15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.11\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.95\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.42\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of VD3 spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e94-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e85-97\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e89-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003eSignal Transduction ， Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353378828346,"sku":"E-EL-0014","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/vd3-vitamin-d3-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21857350058042.jpg?v=1752498642"},{"product_id":"25-hvd3-25-hydroxy-vitamin-d3-elisa-kit","title":"25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit","description":"\u003ch2\u003e25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 241px;\" width=\"210\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 132px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e1.88 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.88 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 25-HVD3 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 25-HVD3 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e9.44\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e28.15\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e88.19\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e9.08\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e27.46\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e85.89\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.62\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.47\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.88\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.52\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.21\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.08\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.57\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.27\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.73\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.41\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.75\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 25-HVD3 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e87-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e86-99\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e95-112\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Area\u003c\/td\u003e\n\u003ctd\u003e Metabolism, Developmental Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353388003386,"sku":"E-EL-0015","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/25-HVD3_25-HydroxyVitaminD3_ELISAKit.jpg?v=1712216043"},{"product_id":"mek-methionine-enkephalin-elisa-kit","title":"MEK(Methionine-Enkephalin) ELISA Kit","description":"\u003ch2\u003eMEK(Methionine-Enkephalin) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 538px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\" style=\"width: 174px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e125-8000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\" style=\"width: 174px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e125-8000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal MEK in samples.No significant cross-reactivity or interference between Universal MEK and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of MEK concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MEK. During the reaction, Universal MEK in the sample or standard competes with a fixed amount of Universal MEK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MEK. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal MEK in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level MEK were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level MEK were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e440.94\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1081.84\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3418.41\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e427.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1013.67\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3096.13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e22.66\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e56.69\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e141.52\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e27.33\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e58.59\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e117.34\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.14\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.24\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.14\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.39\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.78\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.79\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of MEK spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 19px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.04px;\"\u003eDatabase\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px;\"\u003eSwissProt: P01210\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eNeuroscience, Immunology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353483030586,"sku":"E-EL-0020","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/MEK_Methionine-Enkephalin_ELISAKit.jpg?v=1712226326"},{"product_id":"dhvd3-1-25-dihydroxyvitamin-d3-elisa-kit","title":"DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit","description":"\u003ch2\u003eDHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 538px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 174px;\" width=\"170\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e\u003cspan\u003e7.81-500 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 174px;\" width=\"170\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e\u003cspan\u003e4.69 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.81-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.69 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal DHVD3 in samples.No significant cross-reactivity or interference between Universal DHVD3 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of DHVD3 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHVD3. During the reaction, Universal DHVD3 in the sample or standard competes with a fixed amount of Universal DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level DHVD3 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level DHVD3 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e26.85\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e42.69\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e171.01\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e25.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e43.72\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e154.82\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1.47\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.93\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e8.12\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1.29\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.47\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.52\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.75\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.18\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of DHVD3 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e88-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e88-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"width: 538px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\" style=\"width: 174px;\"\u003e\u003cstrong\u003eResearch Area\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003eMetabolism, Developmental Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353501642810,"sku":"E-EL-0016","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/DHVD3_1_25-DihydroxyvitaminD3_ELISAKit.jpg?v=1712227706"},{"product_id":"pgi2-prostacyclin-elisa-kit","title":"PGI2(Prostacyclin) ELISA Kit","description":"\u003ch2\u003ePGI2(Prostacyclin) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 538px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\" style=\"width: 174px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"170\" style=\"width: 174px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 364px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"border-0 pro-table\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal PGI2 in samples.No significant cross-reactivity or interference between Universal PGI2 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of PGI2 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGI2. During the reaction, Universal PGI2 in the sample or standard competes with a fixed amount of Universal PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGI2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGI2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level PGI2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level PGI2 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e94.64\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e246.44\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e963.63\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e90.56\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e267.93\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e1038.28\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e5.82\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e11.68\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e44.91\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e5.8\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e15.86\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e39.56\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e6.15\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e4.74\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e4.66\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e6.4\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e5.92\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e3.81\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of PGI2 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e90-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e91-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e85-96\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 19px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003e Cardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353506066490,"sku":"E-EL-0022","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/pgi2-prostacyclin-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21857505443898.jpg?v=1752498252"},{"product_id":"gsh-glutathione-elisa-kit","title":"GSH(Glutathione) ELISA Kit","description":"\u003ch2\u003eGSH(Glutathione) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 538px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e0.94 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal GSH in samples.No significant cross-reactivity or interference between Universal GSH and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of GSH concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal GSH. During the reaction, Universal GSH in the sample or standard competes with a fixed amount of Universal GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level GSH were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level GSH were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.06\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.81\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e43.81\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.19\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e42.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.82\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.24\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.66\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e1.59\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.34\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.94\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.11\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.85\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.74\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of GSH spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e87-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e88-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e86-99\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 19px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eSignal Transduction, Cell Biology, Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353508294714,"sku":"E-EL-0026","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/GSH_Glutathione_ELISAKit.jpg?v=1712230356"},{"product_id":"8-ohdg-8-hydroxydeoxyguanosine-elisa-kit","title":"8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit","description":"\u003ch2\u003e8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 538px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 8-OHdG in samples.No significant cross-reactivity or interference between Universal 8-OHdG and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-OHdG. During the reaction, Universal 8-OHdG in the sample or standard competes with a fixed amount of Universal 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 8-OHdG were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 8-OHdG were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.79\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e10.11\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e46.98\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.48\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e9.61\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e48.89\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.58\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.69\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.55\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e1.91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.66\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.69\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.95\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 8-OHdG spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e86-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e91-104\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e93-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 19px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003e Metabolism, Cardiovascular, Immunology, Cancer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353508982842,"sku":"E-EL-0028","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/8-OHdG_8-Hydroxydeoxyguanosine_ELISAKit.jpg?v=1712231723"},{"product_id":"ot-oxytocin-elisa-kit","title":"OT(Oxytocin) ELISA Kit","description":"\u003ch2\u003eOT(Oxytocin) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 538px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal OT in samples.No significant cross-reactivity or interference between Universal OT and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of OT concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal OT. During the reaction, Universal OT in the sample or standard competes with a fixed amount of Universal OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal OT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal OT in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level OT were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level OT were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e53.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e153.88\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e495.39\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e56.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e139.06\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e544.58\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.78\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e7.02\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e16.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e2.97\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.04\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e23.85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.23\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.56\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.25\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.34\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.38\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of OT spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e92-104\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e88-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 19px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.04px;\"\u003eDatabase\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px;\"\u003eSwissProt: H:P01178, M:P35454, R:P01179\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eNeuroscience, Biochemicals, Signal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353513570362,"sku":"E-EL-0029","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/OT_Oxytocin_ELISAKit.jpg?v=1712233313"},{"product_id":"dht-dihydrotestosterone-elisa-kit","title":"DHT(Dihydrotestosterone) ELISA Kit","description":"\u003ch2\u003eDHT(Dihydrotestosterone) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 258px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e39.06-2500 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 170.312px; height: 19px;\" data-mce-style=\"width: 170.312px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 352.798px; height: 19px;\" data-mce-style=\"width: 352.798px; height: 19px;\"\u003e\u003cspan\u003e23.44 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e39.06-2500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e23.44 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal DHT in samples.No significant cross-reactivity or interference between Universal DHT and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of DHT concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHT. During the reaction, Universal DHT in the sample or standard competes with a fixed amount of Universal DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHT in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level DHT were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level DHT were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e120.59\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e263\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1006.26\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e109.96\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e261.58\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e989.74\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e8.02\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e14.02\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e32.7\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.86\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e14.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e30.48\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.65\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.33\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.25\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.24\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.46\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.08\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of DHT spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e90-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e90-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e89-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 19px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eSignal Transduction, Developmental Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40353519534138,"sku":"E-EL-0031","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/DHT_Dihydrotestosterone_ELISAKit.jpg?v=1712234809"},{"product_id":"his-histamine-elisa-kit","title":"HIS(Histamine) ELISA Kit","description":"\u003ch2\u003eHIS(Histamine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal HIS in samples.No significant cross-reactivity or interference between Universal HIS and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of HIS concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal HIS. During the reaction, Universal HIS in the sample or standard competes with a fixed amount of Universal HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal HIS in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level HIS were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level HIS were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.17\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.32\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e45.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.54\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e42.52\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.27\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.72\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.51\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.61\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e1.68\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.41\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.35\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.51\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of HIS spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3295px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.824px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e90-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e87-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3295px;\"\u003e89-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.824px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 19px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003e Immunology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354241249338,"sku":"E-EL-0032","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/HIS_Histamine_ELISAKit.jpg?v=1712292236"},{"product_id":"st-5-ht-serotonin-5-hydroxytryptamine-elisa-kit","title":"ST\/5-HT(Serotonin\/5-Hydroxytryptamine) ELISA Kit","description":"\u003ch2\u003eST\/5-HT(Serotonin\/5-Hydroxytryptamine) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e9.38 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal ST\/5-HT in samples.No significant cross-reactivity or interference between Universal ST\/5-HT and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of ST\/5-HT concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST\/5-HT. During the reaction, Universal ST\/5-HT in the sample or standard competes with a fixed amount of Universal ST\/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST\/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST\/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level ST\/5-HT were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level ST\/5-HT were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e51.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e141.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e362.7\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e54.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e132\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e351\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e3.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e15.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e16.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.84\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.18\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.67\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.92\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.59\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of ST\/5-HT spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e95-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e86-99\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e91-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.04px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px; height: 17px;\"\u003e5HT, Enteramine, Thrombocytin, Thrombotonin, 3-(β-Aminoethyl)-5-hydroxyindole\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003e Neuroscience\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354314485818,"sku":"E-EL-0033","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ST5-HT_Serotonin5-Hydroxytryptamine_ELISAKit.jpg?v=1712307204"},{"product_id":"pge2-prostaglandin-e2-elisa-kit","title":"PGE2(Prostaglandin E2) ELISA Kit","description":"\u003ch2\u003ePGE2(Prostaglandin E2) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal PGE2 in samples.No significant cross-reactivity or interference between Universal PGE2 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGE2. During the reaction, Universal PGE2 in the sample or standard competes with a fixed amount of Universal PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level PGE2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level PGE2 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e101.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e236.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e779.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e259.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e728.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e6.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e11.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e42.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e13.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e31.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e6.52\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e5.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.31\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e4.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of PGE2 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e92-109\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e87-99\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e86-96\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.04px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px; height: 17px;\"\u003ePG-E2, Dinoprostone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003e Signal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354431762490,"sku":"E-EL-0034","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/PGE2_ProstaglandinE2_ELISAKit.jpg?v=1712308599"},{"product_id":"3-nt-3-nitrotyrosine-elisa-kit","title":"3-NT(3-Nitrotyrosine) ELISA Kit","description":"\u003ch2\u003e3-NT(3-Nitrotyrosine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"border-0 pro-table\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"170\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 3-NT were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 3-NT were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e5\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e12.5\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e38.3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e5.1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e13.1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e36.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e0.7\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e1.8\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e0.6\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\"\u003e6\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e5.6\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e4.7\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\"\u003e5.88\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\"\u003e4.58\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\"\u003e5.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 3-NT spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e97-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e91-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e93-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.04px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px; height: 17px;\"\u003e 3-Nitro-L-Tyrosine, Nitrotyrosine\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354436055098,"sku":"E-EL-0040","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/3-NT_3-Nitrotyrosine_ELISAKit-1.jpg?v=1712309802"},{"product_id":"8-epi-pgf2-8-epi-prostaglandin-f2-alpha-elisa-kit","title":"8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit","description":"\u003ch2\u003e8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.778px; height: 19px;\" data-mce-style=\"width: 158.778px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.33px; height: 19px;\" data-mce-style=\"width: 137.33px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 8-epi-PGF2α in samples.No significant cross-reactivity or interference between Universal 8-epi-PGF2α and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-epi-PGF2α. During the reaction, Universal 8-epi-PGF2α in the sample or standard competes with a fixed amount of Universal 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 8-epi-PGF2α were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 8-epi-PGF2α were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e51.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e101.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e446.4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e54.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e95.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e463.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e15.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.66\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.42\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.49\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.42\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.39\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 8-epi-PGF2α spiked at three different levels in samples throughout\u003cbr\u003ethe range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e85-97\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e92-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e93-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.04px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.188px; height: 17px;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.04px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.188px;\"\u003eSignal Transduction, Cardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354439659578,"sku":"E-EL-0041","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/8-epi-pgf2-8-epi-prostaglandin-f2-alpha-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21858677129274.jpg?v=1752498645"},{"product_id":"adma-asymmetrical-dimethylarginine-elisa-kit","title":"ADMA(Asymmetrical Dimethylarginine) ELISA Kit","description":"\u003ch2\u003eADMA(Asymmetrical Dimethylarginine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal ADMA in samples.No significant cross-reactivity or interference between Universal ADMA and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ADMA. During the reaction, Universal ADMA in the sample or standard competes with a fixed amount of Universal ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level ADMA were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level ADMA were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e53.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e120.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e371.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e50.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e121.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e371.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e3.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e19.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e19.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.95\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.22\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.19\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.73\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.86\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of ADMA spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e87-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e96-111\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e94-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.054px; height: 17px;\" data-mce-style=\"width: 148.054px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px; height: 17px;\" data-mce-style=\"width: 382.173px; height: 17px;\"\u003eN，N-Dimethylarginine，NG-Dimethylarginine dihydrochloride，2-Amino-5-[(amino-dimethylaminomethylene)amino]pentanoic acid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction, Cardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354466955322,"sku":"E-EL-0042","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ADMA_AsymmetricalDimethylarginine_ELISAKit-1.jpg?v=1712312451"},{"product_id":"epi-epinephrine-adrenaline-elisa-kit","title":"EPI(Epinephrine\/Adrenaline) ELISA Kit","description":"\u003ch2\u003eEPI(Epinephrine\/Adrenaline) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal EPI in samples.No significant cross-reactivity or interference between Universal EPI and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of EPI concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal EPI. During the reaction, Universal EPI in the sample or standard competes with a fixed amount of Universal EPI on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal EPI. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal EPI in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level EPI were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level EPI were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e96.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e321\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e901\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e88.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e307.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e931\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e36\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e13.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e37.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.53\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.21\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.56\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.39\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of EPI spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e88-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e95-109\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3153px;\" data-mce-style=\"height: 19px; width: 97.3153px;\"\u003e89-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.838px;\" data-mce-style=\"height: 19px; width: 205.838px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.054px; height: 17px;\" data-mce-style=\"width: 148.054px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px; height: 17px;\" data-mce-style=\"width: 382.173px; height: 17px;\"\u003eEpinephrine，adrenaline，adrenalin，4，5-β-trihydroxy-N-methylphenethylamine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCell Biology, Metabolism, Epigenetics And Nuclear Signal, Signal Transduction, Neuroscience, Cardiovascular, Cancer, Biochemicals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354475343930,"sku":"E-EL-0045","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/EPI_EpinephrineAdrenaline_ELISAKit-1.jpg?v=1712314258"},{"product_id":"da-dopamine-elisa-kit","title":"DA(Dopamine) ELISA Kit","description":"\u003ch2\u003eDA(Dopamine) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal DA in samples.No significant cross-reactivity or interference between Universal DA and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DA. During the reaction, Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level DA were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level DA were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e93.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e168.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e786.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e94.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e159.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e725\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e7.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e29.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e37\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.55\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.39\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.7\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.63\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.63\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of DA spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e95-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e97-111\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e92-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.054px; height: 17px;\" data-mce-style=\"width: 148.054px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px; height: 17px;\" data-mce-style=\"width: 382.173px; height: 17px;\"\u003e\u003cspan\u003e2-(3，4-dihydroxyphenyl)ethylamine，3，4-dihydroxyphenethylamine，3-hydroxytyramine，DA，Intropin，Revivan，Oxytyramine\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354492678202,"sku":"E-EL-0046","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/DA_Dopamine_ELISAKit-1.jpg?v=1712315427"},{"product_id":"na-ne-noradrenaline-norepinephrine-elisa-kit","title":"NA\/NE(Noradrenaline\/Norepinephrine) ELISA Kit","description":"\u003ch2\u003eNA\/NE(Noradrenaline\/Norepinephrine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.31-20 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.19 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.31-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.19 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal NA\/NE in samples.No significant cross-reactivity or interference between Universal NA\/NE and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of NA\/NE concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal NA\/NE. During the reaction, Universal NA\/NE in the sample or standard competes with a fixed amount of Universal NA\/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal NA\/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal NA\/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level NA\/NE were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level NA\/NE were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e9.5\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e9.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e0.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e9.09\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.35\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3.16\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.17\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e4.17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of NA\/NE spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e88-99\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e94-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e89-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 148.054px; height: 17px;\" data-mce-style=\"width: 148.054px; height: 17px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px; height: 17px;\" data-mce-style=\"width: 382.173px; height: 17px;\"\u003eNorepinephrine，NE，noradrenaline，NA，NAd，norad，4，5-β-trihydroxyphenethylamine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience ， Cardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354498904122,"sku":"E-EL-0047","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/NANE_NoradrenalineNorepinephrine_ELISAKi-1t.jpg?v=1712316774"},{"product_id":"fmn-flavin-mononucleotide-elisa-kit","title":"FMN(Flavin mononucleotide) ELISA Kit","description":"\u003ch2\u003eFMN(Flavin mononucleotide) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal FMN in samples.No significant cross-reactivity or interference between Universal FMN and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of FMN concentrations in ..\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal FMN. During the reaction, Universal FMN in the sample or standard competes with a fixed amount of Universal FMN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FMN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal FMN in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level FMN were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level FMN were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e54.07\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e150.26\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e353.12\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e49.95\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e141.68\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e339.22\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.97\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e8.61\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e12.64\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3.25\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.89\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e12.45\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.49\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.73\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.58\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.51\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.86\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.67\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of FMN spiked at three different levels in samples throughout the\u003cbr data-mce-fragment=\"1\"\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e89-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e87-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e93-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354501853242,"sku":"E-EL-0049","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/FMN_Flavinmononucleotide_ELISAKi-1t.jpg?v=1712317986"},{"product_id":"aa-arachidonic-acid-elisa-kit","title":"AA(Arachidonic Acid) ELISA Kit","description":"\u003ch2\u003eAA(Arachidonic Acid) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal AA in samples.No significant cross-reactivity or interference between Universal AA and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of AA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal AA. During the reaction, Universal AA in the sample or standard competes with a fixed amount of Universal AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal AA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal AA in tested samples can be calculated by comparing the OD of the samples to the standard curve. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level AA were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level AA were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e9.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e36.7\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e8.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e36.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.8\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.38\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.26\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of AA spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e87-98\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e93-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e90-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003eARA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40354506932282,"sku":"E-EL-0051","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/AA_ArachidonicAcid_ELISAKit-1.jpg?v=1712319915"},{"product_id":"lxa4-lipoxin-a4-elisa-kit","title":"LXA4(Lipoxin A4) ELISA Kit","description":"\u003ch2\u003eLXA4(Lipoxin A4) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.78-50 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.47 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.78-50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.47 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal LXA4 in samples.No significant cross-reactivity or interference between Universal LXA4 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of LXA4 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LXA4. During the reaction, Universal LXA4 in the sample or standard competes with a fixed amount of Universal LXA4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LXA4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LXA4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level LXA4 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level LXA4 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e2.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e2.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e0.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e0.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e3.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3.92\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.93\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e3.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.08\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3.92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of LXA4 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e92-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e91-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e94-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003e ASPIRIN-TRIGGERED LXA4, LXA4-15-EPI, LIPOXIN A4，15-EPI\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCell Biology, Immunology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355360800826,"sku":"E-EL-0053","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/LXA4_LipoxinA4_ELISAKi-1t.jpg?v=1712381117"},{"product_id":"6-keto-pgf1a-6-keto-prostaglandin-f1a-elisa-kit","title":"6-keto-PGF1a(6-keto-prostaglandin F1a) ELISA Kit","description":"\u003ch2\u003e6-keto-PGF1a(6-keto-prostaglandin F1a) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 6-keto-PGF1a in samples.No significant cross-reactivity or interference between Universal 6-keto-PGF1a and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 6-keto-PGF1a concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 6-keto-PGF1a. During the reaction, Universal 6-keto-PGF1a in the sample or standard competes with a fixed amount of Universal 6-keto-PGF1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 6-keto-PGF1a. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 6-keto-PGF1a in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 6-keto-PGF1a were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 6-keto-PGF1a were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e54.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e139.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e366.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e50.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e151.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e402.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e3.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e17.9\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e14.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.62\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.08\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.89\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.94\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.29\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 6-keto-PGF1a spiked at three different levels in samples throughout\u003cbr\u003ethe range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e91-104\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e83-97\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e87-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355426140218,"sku":"E-EL-0054","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/6-keto-PGF1a_6-keto-prostaglandinF1a_ELISAKit-1.jpg?v=1712382191"},{"product_id":"camp-cyclic-adenosine-monophosphate-elisa-kit","title":"cAMP(Cyclic adenosine monophosphate) ELISA Kit","description":"\u003ch2\u003ecAMP(Cyclic adenosine monophosphate) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal cAMP in samples.No significant cross-reactivity or interference between Universal cAMP and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cAMP. During the reaction, Universal cAMP in the sample or standard competes with a fixed amount of Universal cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level cAMP were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level cAMP were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e14.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e37.4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e5.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e15\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e34.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e0.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e0.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e1.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.56\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.96\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.35\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e6.78\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e5.16\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of cAMP spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e89-104\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e86-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e87-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003eCyclic adenosine monophosphate，cAMP，cyclic AMP，3'-5'-cyclic adenosine monophosphate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355429089338,"sku":"E-EL-0056","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/cAMP_Cyclicadenosinemonophosphate_ELISAKit-1.jpg?v=1712383368"},{"product_id":"txa2-thromboxane-a2-elisa-kit","title":"TXA2(Thromboxane A2) ELISA Kit","description":"\u003ch2\u003eTXA2(Thromboxane A2) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal TXA2 in samples.No significant cross-reactivity or interference between Universal TXA2 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of TXA2 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal TXA2. During the reaction, Universal TXA2 in the sample or standard competes with a fixed amount of Universal TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level TXA2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level TXA2 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e50.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e134.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e401\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e49.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e147.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e433.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e7.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e15.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e7.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e16.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.11\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.57\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.89\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.11\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of TXA2 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e94-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e94-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e86-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 534px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 144.645px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 374.46px;\"\u003eTXA2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCardiovascular, Metabolism, Signal Transduction, Cancer, Epigenetics And Nuclear Signal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355430170682,"sku":"E-EL-0057","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/TXA2_ThromboxaneA2_ELISAKit-1.jpg?v=1712384517"},{"product_id":"cr-creatinine-elisa-kit","title":"Cr(Creatinine) ELISA Kit","description":"\u003ch2\u003eCr(Creatinine) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e1.25-80 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.75 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.25-80 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.75 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal Cr in samples.No significant cross-reactivity or interference between Universal Cr and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of Cr concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Cr. During the reaction, Universal Cr in the sample or standard competes with a fixed amount of Universal Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Cr. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Cr in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level Cr were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level Cr were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e3.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e10.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e34.8\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e11.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e31.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e1.8\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e1.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.13\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.67\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.17\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e7.14\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.38\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of Cr spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e88-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e92-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.3011px;\" data-mce-style=\"height: 19px; width: 97.3011px;\"\u003e94-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.852px;\" data-mce-style=\"height: 19px; width: 205.852px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 534px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 144.645px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 374.46px;\"\u003e 2-Amino-1-methyl-5H-imidazol-4-one，2-Amino-1-methylimidazol-4-ol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCancer ， Neuroscience\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355500654650,"sku":"E-EL-0058","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Cr_Creatinine_ELISAKit-1.jpg?v=1712386041"},{"product_id":"ip3-inositol-triphosphate-elisa-kit","title":"IP3(Inositol Triphosphate) ELISA Kit","description":"\u003ch2\u003eIP3(Inositol Triphosphate) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal IP3 in samples.No significant cross-reactivity or interference between Universal IP3 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal IP3. During the reaction, Universal IP3 in the sample or standard competes with a fixed amount of Universal IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level IP3 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level IP3 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e54.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e130.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e426.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e58.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e132.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e424.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e16.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e7.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e14.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.96\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.99\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.8\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.85\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.82\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.51\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of IP3 spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e88-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e88-98\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e85-101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 144px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 378px;\"\u003eInositol trisphosphate，inositol 1，4，5-trisphosphate，triphosphoinositol，InsP3，Ins3P，IP3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eBiochemicals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355552952378,"sku":"E-EL-0059","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/IP3_InositolTriphosphate_ELISAKit-1.jpg?v=1712386982"},{"product_id":"mda-malondialdehyde-elisa-kit","title":"MDA(Malondialdehyde) ELISA Kit","description":"\u003ch2\u003eMDA(Malondialdehyde) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e18.75 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal MDA in samples.No significant cross-reactivity or interference between Universal MDA and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MDA. During the reaction, Universal MDA in the sample or standard competes with a fixed amount of Universal MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level MDA were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level MDA were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e109.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e308.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e848.8\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e111.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e321\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e892.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e6.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e17.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e37.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e17\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e46.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.57\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.39\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e6.28\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e5.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of MDA spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e92-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e94-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e93-110\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003e Neuroscience ， Signal Transduction ， Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40355600433210,"sku":"E-EL-0060","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/MDA_Malondialdehyde_ELISAKi-1t.jpg?v=1712388053"},{"product_id":"ltb4-leukotriene-b4-elisa-kit","title":"LTB4(Leukotriene B4) ELISA Kit","description":"\u003ch2\u003eLTB4(Leukotriene B4) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal LTB4 in samples.No significant cross-reactivity or interference between Universal LTB4 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTB4. During the reaction, Universal LTB4 in the sample or standard competes with a fixed amount of Universal LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTB4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTB4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level LTB4 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level LTB4 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e54.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e119.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e336.5\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e50.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e116.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e318.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e3.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e18.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e2.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e15.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e6.45\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.19\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.41\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e5.56\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.12\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e4.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of LTB4 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e89-105\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e86-98\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e93-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003e\u003cspan\u003eLeukotriene B4\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eCell Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40356018159674,"sku":"E-EL-0061","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/LTB4_LeukotrieneB4_ELISAKit-1.jpg?v=1712395822"},{"product_id":"pgd2-prostaglandin-d2-elisa-kit","title":"PGD2(Prostaglandin D2) ELISA Kit","description":"\u003ch2\u003ePGD2(Prostaglandin D2) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal PGD2 in samples.No significant cross-reactivity or interference between Universal PGD2 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of PGD2 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGD2. During the reaction, Universal PGD2 in the sample or standard competes with a fixed amount of Universal PGD2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGD2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGD2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level PGD2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level PGD2 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e49.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e101.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e404.9\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e46.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e103.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e364.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e2.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e12.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e3.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e13.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.25\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.81\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2.99\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e6.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.69\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3.59\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of PGD2 spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e91-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e85-96\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e87-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40356280664122,"sku":"E-EL-0066","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/PGD2_ProstaglandinD2_ELISAKit-1.jpg?v=1712399083"},{"product_id":"sp-substance-p-elisa-kit","title":"SP(Substance P) ELISA Kit","description":"\u003ch2\u003eSP(Substance P) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e78.13-5000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e46.88 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e78.13-5000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e46.88 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal SP in samples.No significant cross-reactivity or interference between Universal SP and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal SP. During the reaction, Universal SP in the sample or standard competes with a fixed amount of Universal SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level SP were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level SP were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e233.17\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e510.94\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2017.45\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e206.77\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e515.17\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e2371.94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e12.87\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e23.61\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e104.91\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e12.18\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e23.85\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e125.24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.52\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.62\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.89\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.63\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.28\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of SP spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e86-96\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e99-109\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e90-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eDatabase\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003eSwissProt: H:P20366 M:P41539 R:P06767\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience ， Cardiovascular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40356764418106,"sku":"E-EL-0067","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/SP_SubstanceP_ELISAKit-1.jpg?v=1712399975"},{"product_id":"car-carnosine-elisa-kit","title":"Car(Carnosine) ELISA Kit","description":"\u003ch2\u003eCar(Carnosine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal Car in samples.No significant cross-reactivity or interference between Universal Car and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of Car concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Car. During the reaction, Universal Car in the sample or standard competes with a fixed amount of Universal Car on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Car. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Car in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level Car were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level Car were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e42.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e86.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e319.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e41\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e78.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e299\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e16\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e2.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e10.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.07\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.86\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.01\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.37\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.84\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3.41\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of Car spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e88-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e85-97\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.662px;\" data-mce-style=\"height: 19px; width: 221.662px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2869px;\" data-mce-style=\"height: 19px; width: 97.2869px;\"\u003e90-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.653px;\" data-mce-style=\"height: 19px; width: 205.653px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003e\u003cspan\u003ebeta-alanyl-L-histidine\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction, Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40356923179066,"sku":"E-EL-0069","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Car_Carnosine_ELISAKit-1.jpg?v=1712401081"},{"product_id":"ald-aldosterone-elisa-kit","title":"ALD(Aldosterone) ELISA Kit","description":"\u003ch2\u003eALD(Aldosterone) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal ALD in samples.No significant cross-reactivity or interference between Universal ALD and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of ALD concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ALD. During the reaction, Universal ALD in the sample or standard competes with a fixed amount of Universal ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ALD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ALD in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level ALD were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level ALD were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e51.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e89.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e476.9\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e46.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e94.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e479.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e2.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e3.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e23\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.21\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.19\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e6.84\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.05\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of ALD spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e86-98\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e96-112\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e90-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40357088395322,"sku":"E-EL-0070","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ALD_Aldosterone_ELISAKit-1.jpg?v=1712402132"},{"product_id":"gnrh-gonadotropin-releasing-hormone-elisa-kit","title":"GnRH(Gonadotropin Releasing Hormone) ELISA Kit","description":"\u003ch2\u003eGnRH(Gonadotropin Releasing Hormone) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal GnRH in samples.No significant cross-reactivity or interference between Universal GnRH and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal GnRH. During the reaction, Universal GnRH in the sample or standard competes with a fixed amount of Universal GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level GnRH were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level GnRH were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e281.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e937.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2978.3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e281.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e856.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3080.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e14.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e48.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e131\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e18\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e41.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e114\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.19\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e6.39\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.8\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of GnRH spiked at three different levels in samples throughout the\u003cbr\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e93-111\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e88-100\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e87-97\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eDatabase\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003eSwissProt: P01148\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 148.054px;\"\u003eSyonoyms\u003c\/td\u003e\n\u003ctd style=\"width: 382.173px;\"\u003eGNRH1，GRH，HH12，LHRH，LNRH，Luliberin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eDevelopmental Biology ， Signal Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40357272485946,"sku":"E-EL-0071","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/GnRH_GonadotropinReleasingHormone_ELISAKi-1t.jpg?v=1712403277"},{"product_id":"5-hiaa-5-hydroxyindoleacetic-acid-elisa-kit","title":"5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit","description":"\u003ch2\u003e5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.94 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal 5-HIAA in samples.No significant cross-reactivity or interference between Universal 5-HIAA and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 5-HIAA. During the reaction, Universal 5-HIAA in the sample or standard competes with a fixed amount of Universal 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HIAA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level 5-HIAA were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level 5-HIAA were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable width=\"448\" style=\"height: 154px;\" data-mce-style=\"height: 154px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 229.006px;\" data-mce-style=\"width: 229.006px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd colspan=\"3\" style=\"width: 225.028px;\" data-mce-style=\"width: 225.028px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e204.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e588.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e1720.2\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e221.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e588.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e1852\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e10.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e29.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e61.9\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e13.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e24.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e59.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-style=\"width: 74.6023px;\" data-mce-fragment=\"1\"\u003e5.19\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e3.6\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-style=\"width: 70.5682px;\" data-mce-fragment=\"1\"\u003e6.29\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-style=\"width: 71.5767px;\" data-mce-fragment=\"1\"\u003e4.2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-style=\"width: 71.6335px;\" data-mce-fragment=\"1\"\u003e3.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of 5-HIAA spiked at three different levels in samples throughout the\u003cbr data-mce-fragment=\"1\"\u003erange of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable style=\"height: 76px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e85-98\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e93-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e92-106\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience, Metabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40357507825722,"sku":"E-EL-0075","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/5-HIAA_5-HydroxyindoleaceticAcid_ELISAKit-1.jpg?v=1712404525"},{"product_id":"bb-bilirubin-elisa-kit","title":"Bb(Bilirubin) ELISA Kit","description":"\u003ch2\u003eBb(Bilirubin) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.31-20 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.19 μg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.31-20 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.19 μg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal Bb in samples.No significant cross-reactivity or interference between Universal Bb and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of Bb concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Bb. During the reaction, Universal Bb in the sample or standard competes with a fixed amount of Universal Bb on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Bb. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Bb in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level Bb were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level Bb were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.9\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e11.5\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e45.4\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e10.6\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e48.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.7\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.1\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.3\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.4\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e2.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e6.12\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e6.09\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.63\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e5.88\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3.77\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e5.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of Bb spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e87-103\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e88-102\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e90-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eMetabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40357664751674,"sku":"E-EL-0076","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/bb-bilirubin-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21860616273978.jpg?v=1752501193"},{"product_id":"ft3-free-triiodothyronine-elisa-kit","title":"fT3(Free Triiodothyronine) ELISA Kit","description":"\u003ch2\u003efT3(Free Triiodothyronine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 311px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e1.56-100 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.94 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"border-0 pro-table\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003esize\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Method\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eColormetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eDetection Range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.56-100 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.94 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSample Type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes Universal fT3 in samples.No significant cross-reactivity or interference between Universal fT3 and analogues was observed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003ePrecision\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eRecovery\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80%-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eIntroduction\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of fT3 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eApplications\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"170\" data-mce-fragment=\"1\"\u003eStorage\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2-8℃\/-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal fT3. During the reaction, Universal fT3 in the sample or standard competes with a fixed amount of Universal fT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal fT3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal fT3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and\u003cbr\u003ehigh level fT3 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and\u003cbr\u003ehigh level fT3 were tested on 3 different plates, 20 replicates in each plate,\u003cbr\u003erespectively.\u003c\/p\u003e\n\u003ctable style=\"height: 154px;\" width=\"448\" data-mce-fragment=\"1\" data-mce-style=\"height: 154px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 229.006px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 229.006px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 225.028px;\" colspan=\"3\" data-mce-fragment=\"1\" data-mce-style=\"width: 225.028px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003en\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e4.16\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e12.43\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e47.53\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.84\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e11.65\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e49.47\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e0.23\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.59\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e2.44\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e0.24\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e0.57\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e2.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCV (%)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 74.6023px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 74.6023px;\"\u003e5.53\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.75\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e5.13\u003c\/td\u003e\n\u003ctd style=\"width: 70.5682px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 70.5682px;\"\u003e4.96\u003c\/td\u003e\n\u003ctd style=\"width: 71.5767px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.5767px;\"\u003e4.89\u003c\/td\u003e\n\u003ctd style=\"width: 71.6335px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 71.6335px;\"\u003e4.85\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of fT3 spiked at three different levels in samples throughout the range\u003cbr\u003eof the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 76px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e\u003cb\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e\u003cb\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e88-95\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e96-108\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 221.449px;\" data-mce-style=\"height: 19px; width: 221.449px;\"\u003e\u003cb\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 97.2727px;\" data-mce-style=\"height: 19px; width: 97.2727px;\"\u003e101-107\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 205.881px;\" data-mce-style=\"height: 19px; width: 205.881px;\"\u003e104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 522px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignaling Transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40357804900410,"sku":"E-EL-0079","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/fT3_FreeTriiodothyronine_ELISAKit-1.jpg?v=1712406395"},{"product_id":"ach-acetylcholine-elisa-kit","title":"ACH(Acetylcholine) ELISA Kit ","description":"\u003ch2\u003eACH(Acetylcholine) ELISA Kit \u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e15.63-1000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e9.38 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.38 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes ACH in samples. No significant cross-reactivity or interference between ACH and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of ACH concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACH. During the reaction, ACH in the sample or standard competes with a fixed amount of ACH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACH in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ACH were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ACH were tested on 3 different plates, 20 replicates in each plate.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e124.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e438.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e55.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e116.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e429.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e13.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.11\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.91\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.33\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.89\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.61\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of ACH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e89-102\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e88-102\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e87-102\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience, Signal transduction, Immunology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"lct\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp data-mce-fragment=\"1\" class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363027103802,"sku":"E-EL-0081","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ACH_Acetylcholine_ELISAKit-1.jpg?v=1712639907"},{"product_id":"cgmp-cyclic-gmp-elisa-kit","title":"cGMP(Cyclic GMP) ELISA Kit","description":"\u003ch2\u003ecGMP(Cyclic GMP) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.78-50 pmol\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.47 pmol\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.78-50 pmol\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.47 pmol\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes cGMP in samples. No significant cross-reactivity or interference between cGMP and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level cGMP were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level cGMP were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (pmol\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e98.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e299.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e999.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e326.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1055.70\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e13.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e35.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e53.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.28\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.19\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of cGMP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91-104\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91-108\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-97\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignal transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"lct\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp data-mce-fragment=\"1\" class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" align=\"left\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" data-mce-src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363084218426,"sku":"E-EL-0083","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/cgmp-cyclic-gmp-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21864573337658.jpg?v=1752501348"},{"product_id":"p5-pregnenolone-elisa-kit","title":"P5(Pregnenolone) ELISA Kit","description":"\u003ch2\u003eP5(Pregnenolone) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 311px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.78-50 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.47 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.78-50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.47 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes P5 in samples. No significant cross-reactivity or interference between P5 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of P5 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with P5. During the reaction, P5 in the sample or standard competes with a fixed amount of P5 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to P5. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P5 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level P5 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level P5 were tested on 3 different plates, 20 replicates in each plate.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e206.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e548.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1704.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e189.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e527.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1854.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e12.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e25.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e56.20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e12.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e23.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96.40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.71\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.59\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.49\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of P5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96-108\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-99\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e85-95\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eNeuroscience, Developmental biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363096997946,"sku":"E-EL-0086","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/p5-pregnenolone-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21864582742074.jpg?v=1752498487"},{"product_id":"15d-pgj2-15-deoxy-delta12-14-prostaglandin-j2-elisa-kit","title":"15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit","description":"\u003ch2\u003e15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 295px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e3.13-200 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e1.88 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.88 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 15d-PGJ2 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 15d-PGJ2 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e422.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e851.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2917.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e453.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e842.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3029.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e28.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e40.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e116.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e26.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e43.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e160.60\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.71\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of 15d-PGJ2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92-106\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e94-107\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93-106\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 522px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 148.054px;\" data-mce-style=\"height: 19px; width: 148.054px;\"\u003e\u003cspan\u003eSynonyms\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003e\u003cspan\u003e15-d-Δ12,14-PGJ2\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363104075834,"sku":"E-EL-0087","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/15d-PGJ2_15-Deoxy-Delta12_14-prostaglandinJ2_ELISAKit-1.jpg?v=1712642511"},{"product_id":"ptd-pentosidine-elisa-kit","title":"PTD(Pentosidine) ELISA Kit","description":"\u003ch2\u003ePTD(Pentosidine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 295px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.78-50 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e0.47 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.78-50 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.47 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes PTD in samples. No significant cross-reactivity or interference between PTD and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of PTD concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PTD. During the reaction, PTD in the sample or standard competes with a fixed amount of PTD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PTD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTD in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PTD were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PTD were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e46.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e106.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e442.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e44.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e106.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e426.90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.60\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e23.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e21.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.62\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.81\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.23\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.41\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of PTD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92-106\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e89-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-97\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 512.5px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 130.5px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eMetabolism\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363145494586,"sku":"E-EL-0091","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/PTD_Pentosidine_ELISAKit-1.jpg?v=1712643469"},{"product_id":"t4-thyroxine-elisa-kit","title":"T4(Thyroxine) ELISA Kit","description":"\u003ch2\u003eT4(Thyroxine) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 295px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e3.13-200 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e1.88 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.88 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes T4 in samples. No significant cross-reactivity or interference between T4 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of T4 concentrations in .\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T4. During the reaction, T4 in the sample or standard competes with a fixed amount of T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T4 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T4 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.52\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e19.65\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e87.25\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.82\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e19.15\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e88.57\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.59\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.13\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.62\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.68\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.83\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.36\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.75\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.92\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.33\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.79\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of T4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e94-108\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e101\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e90-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e97\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e88-104\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 512.5px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 130.5px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003eSignaling transduction,Developmental Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363155161146,"sku":"E-EL-0100","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/t4-thyroxine-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21864640217146.jpg?v=1752501233"},{"product_id":"bh4-tetrahydrobiopterin-elisa-kit","title":"BH4(Tetrahydrobiopterin) ELISA Kit","description":"\u003ch2\u003eBH4(Tetrahydrobiopterin) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 295px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\/48T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes BH4 in samples. No significant cross-reactivity or interference between BH4 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of BH4 concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BH4. During the reaction, BH4 in the sample or standard competes with a fixed amount of BH4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BH4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BH4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BH4 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BH4 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e105.95\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e238.37\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e995.86\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e112.62\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e233.33\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1067.77\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.79\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e13.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50.39\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.27\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e13.91\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50.83\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.41\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.62\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.06\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.57\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.96\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.76\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of BH4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e89-104\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e88-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93-108\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 510px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 128px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003e\u003cspan\u003eTHB, sapropterin\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"dt_tab_citation\" class=\"dt01\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363159126074,"sku":"E-EL-0110","price":679.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/bh4-tetrahydrobiopterin-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21864642609210.jpg?v=1752501150"},{"product_id":"bh2-dihydrobiopterin-elisa-kit","title":"BH2(Dihydrobiopterin) ELISA Kit","description":"\u003ch2\u003eBH2(Dihydrobiopterin) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 295px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes BH2 in samples. No significant cross-reactivity or interference between BH2 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of BH2 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BH2. During the reaction, BH2 in the sample or standard competes with a fixed amount of BH2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BH2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BH2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BH2 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BH2 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e110.11\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e316.37\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e963.82\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e106.55\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e327.65\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e982.41\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.22\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e15.91\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e48.87\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e7.06\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e16.64\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e35.76\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.56\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.03\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.07\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.63\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.08\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.64\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of BH2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92-108\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e85-100\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e87-99\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 510px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 128px;\" width=\"25%\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 382.173px;\" data-mce-style=\"height: 19px; width: 382.173px;\"\u003e7,8-Dihydrobiopterin\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab_citation\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363169972282,"sku":"E-EL-0111","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/BH2_Dihydrobiopterin_ELISAKit-1.jpg?v=1712646503"},{"product_id":"ltd4-leukotriene-d4-elisa-kit","title":"LTD4(Leukotriene D4) ELISA Kit","description":"\u003ch2\u003eLTD4(Leukotriene D4) ELISA Kit\u003c\/h2\u003e\n\u003ctable style=\"width: 295px; height: 38px;\" class=\"border-0 pro-table\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.31-20 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e0.19 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.31-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.19 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes LTD4 in samples. No significant cross-reactivity or interference between LTD4 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of LTD4 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTD4. During the reaction, LTD4 in the sample or standard competes with a fixed amount of LTD4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTD4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTD4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"626\" style=\"font-size: 0.875rem; height: 353px;\" data-mce-style=\"font-size: 0.875rem; height: 353px;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 114px;\" rowspan=\"6\" data-mce-style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 38px;\" rowspan=\"2\" data-mce-style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 67px;\" rowspan=\"4\" data-mce-style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 10px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-style=\"height: 39px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-style=\"height: 19px;\" data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level LTD4 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level LTD4 were tested on 3 different plates, 20 replicates in each plate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.23\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of LTD4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-97\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-100\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e93\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e84-99\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 36px; width: 499px;\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 128px;\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 371px;\"\u003eLTD-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"dt_tab_citation\" class=\"dt01\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363175084090,"sku":"E-EL-0113","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/ltd4-leukotriene-d4-elisa-kit-default-title-elabscience-biotechnology-cell-assays-21864663679034.jpg?v=1752501244"},{"product_id":"lte4-leukotriene-e4-elisa-kit","title":"LTE4(Leukotriene E4) ELISA Kit","description":"\u003ch2\u003eLTE4(Leukotriene E4) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 295px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e31.25-2000 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e18.75 pg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e31.25-2000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e18.75 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSerum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes LTE4 in samples. No significant cross-reactivity or interference between LTE4 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of LTE4 concentrations in Serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTE4. During the reaction, LTE4 in the sample or standard competes with a fixed amount of LTE4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTE4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTE4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\" style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 10px;\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 10px;\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 10px;\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 39px;\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 39px;\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 39px;\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 39px;\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 125.44px; height: 19px;\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\" style=\"height: 19px;\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 233.679px; height: 19px;\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" style=\"width: 165.483px; height: 19px;\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level LTE4 were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level LTE4 were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\" colspan=\"3\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (pg\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e101.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e209.70\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e903.10\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e99.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e225.50\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e937.60\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e9.40\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e34.30\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.00\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e12.20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e30.90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.33\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.48\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.80\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.03\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5.41\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eThe recovery of LTE4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable data-mce-fragment=\"1\" class=\"dtl_table2 table_line\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e86-97\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e84-98\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e91\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e92-110\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 488px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 128px;\" width=\"25%\"\u003eSynonyms\u003c\/td\u003e\n\u003ctd style=\"width: 360px;\"\u003eLTE4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" width=\"30%\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\" alt=\"elisa assay procedure 1\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\" class=\"show\" alt=\"elisa assay procedure 2\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\" class=\"show\" alt=\"elisa assay procedure 3\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\" class=\"show\" alt=\"elisa assay procedure 4\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\" class=\"show\" alt=\"elisa assay procedure 5\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\" class=\"move\"\u003e\u003cimg src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\" class=\"show\" alt=\"elisa assay procedure 6\"\u003e\u003c\/td\u003e\n\u003ctd align=\"left\" valign=\"middle\" class=\"move\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab_citation\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363289247802,"sku":"E-EL-0114","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/LTE4_LeukotrieneE4_ELISAKit-1.jpg?v=1712655551"},{"product_id":"dhea-dehydroepiandrosterone-elisa-kit","title":"DHEA(Dehydroepiandrosterone) ELISA Kit","description":"\u003ch2\u003eDHEA(Dehydroepiandrosterone) ELISA Kit\u003c\/h2\u003e\n\u003ctable class=\"border-0 pro-table\" style=\"width: 295px; height: 38px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137.344px; height: 19px;\" data-mce-style=\"width: 137.344px; height: 19px;\"\u003e\u003cspan\u003e0.16-10 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 158.764px; height: 19px;\" data-mce-style=\"width: 158.764px; height: 19px;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 137px;\"\u003e\u003cspan\u003e0.10 ng\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ch3\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay type\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eFormat\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eAssay time\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2.0h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eDetection range\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.16-10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSample type \u0026amp;Sample volume\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eserum, plasma and other biological fluids; 50μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eSpecificity\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis kit recognizes DHEA in samples. No significant cross-reactivity or interference between DHEA and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eReproducibility\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eBoth intra-CV and inter-CV are \u0026lt; 10%.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd width=\"30%\" data-mce-fragment=\"1\"\u003eApplication\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eThis ELISA kit applies to the in vitro quantitative determination of DHEA concentrations in serum, plasma and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cbr\u003eTest Principle\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA. During the reaction, DHEA in the sample or standard competes with a fixed amount of DHEA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAn unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"font-size: 0.875rem; height: 353px;\" width=\"626\" data-mce-fragment=\"1\" data-mce-style=\"font-size: 0.875rem; height: 353px;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"text-align: center; width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 233.679px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 165.483px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"text-align: center; width: 125.44px; height: 19px;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eMicro ELISA Plate(Dismountable)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 8 wells ×12 strips\u003c\/td\u003e\n\u003ctd rowspan=\"6\" style=\"width: 125.44px; height: 114px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 114px;\"\u003e-20℃, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 8 wells ×6 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eReference Standard\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated Biotinylated Detection Ab (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 233.679px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 38px;\"\u003eConcentrated HRP Conjugate (100×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e96T: 1 vial, 120 μL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 125.44px; height: 38px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 38px;\"\u003e-20℃(Protect from\u003cbr data-mce-fragment=\"1\"\u003elight), 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e48T: 1 vial, 60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eReference Standard \u0026amp; Sample Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 20 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 125.44px; height: 67px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 67px;\"\u003e2-8°C, 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 10px;\"\u003eBiotinylated Detection Ab Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 10px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 10px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eHRP Conjugate Diluent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 14 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eConcentrated Wash Buffer (25×)\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 39px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 39px;\"\u003eSubstrate Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 39px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 39px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 39px;\"\u003e2-8℃ (Protect from light)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 vial, 10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e5 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eManual\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd style=\"width: 125.44px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 125.44px; height: 19px;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 233.679px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 233.679px; height: 19px;\"\u003eCertificate of Analysis\u003c\/td\u003e\n\u003ctd style=\"width: 165.483px; height: 19px;\" data-mce-fragment=\"1\" data-mce-style=\"width: 165.483px; height: 19px;\"\u003e1 copy\u003c\/td\u003e\n\u003ctd\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eTechnical Data:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level DHEA were tested 20 times on one plate, respectively.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level DHEA were tested on 3 different plates, 20 replicates in each plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e \u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntra-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003cth colspan=\"3\" data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eInter-assay Precision\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e2\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003en\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMean (ng\/mL)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.46\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.02\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.54\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.42\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1.07\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStandard deviation\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.03\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.05\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.22\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.02\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.05\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e0.17\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCV (%)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e6.52\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.90\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.85\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.76\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.67\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e4.06\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003cstrong style=\"font-size: 0.875rem;\"\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"\"\u003e\n\u003cdiv class=\"\"\u003e\n\u003cp\u003eThe recovery of DHEA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ctable class=\"dtl_table2 table_line\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSample Type\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRange (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003cth data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAverage Recovery (%)\u003c\/b\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eSerum(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e96-103\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eEDTA plasma(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e87-102\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eCell culture media(n=8)\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e89-101\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2 table_line\" style=\"height: 36px; width: 482px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" style=\"width: 122px;\"\u003eResearch Area\u003c\/td\u003e\n\u003ctd style=\"width: 360px;\"\u003eMetabolism, Neuroscience, Neuroscience\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay Procedures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"dt_tab4\" class=\"dt01\"\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab4\"\u003e\n\u003ctable class=\"lct\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\" class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 1\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_1.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\"\u003e\n\u003cspan class=\"first_frame\"\u003e\u003c\/span\u003e\n\u003cp class=\"first\"\u003e1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 2\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_2.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e2. Aspirate and wash the plate for 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 3\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_3.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 4\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_4.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\"\u003e\u003cimg alt=\"elisa assay procedure 5\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_5.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\n\u003cp\u003e5. Add 50μL Stop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"move\" align=\"left\"\u003e\u003cimg alt=\"elisa assay procedure 6\" class=\"show\" src=\"https:\/\/file.elabscience.com\/Public\/Home\/pictures\/elisa_competitive\/lct_6.jpg\"\u003e\u003c\/td\u003e\n\u003ctd class=\"move\" valign=\"middle\" align=\"left\"\u003e\n\u003cp\u003e6. Read the plate at 450nm immediately. Calculation of the results\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"dt01\" id=\"dt_tab_citation\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"Default Title","offer_id":40363299405882,"sku":"E-EL-0115","price":552.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/DHEA_Dehydroepiandrosterone_ELISAKit-1.jpg?v=1712656759"}],"url":"https:\/\/www.msesupplies.com\/en-de\/collections\/elisa-kits.oembed?page=77","provider":"MSE Supplies","version":"1.0","type":"link"}