{"title":"Elabscience Cell Function Assays","description":"","products":[{"product_id":"one-step-rapid-dewaxing-solution","title":"One-step Rapid Dewaxing Solution","description":"\u003ch2\u003eOne-step Rapid Dewaxing Solution\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eElabscience® one step rapid dewaxing solution is a rapid reagent for paraffin slides pretreatment. There is no need to do the traditional three times of xylene dewaxing, three to five times of gradient ethanol hydration, but only one Dewaxing solution. The operation time is effectively shortened, the cumbersome operation steps are simplified so the variables in operation are reduced, and the stability is improved. The Dewaxing solution applies new environmental protection technology is not only reduces the harm to the body, but also cause no pollution to the environment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDewaxing solution (10×) is a 10× concentrated solution. Dilute with ddH\u003csub\u003e2\u003c\/sub\u003e0 to 1 ×Dewaxing working solution before use.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental Operation Steps\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Dilute the dewaxing solution (10×) with ddH2O at the ratio of 1:9.\u003cbr\u003e\u003cbr\u003eNote: Flocculent sediment will be generated during dilution. Do not blow it with any pipette. Please bake the diluted solution at 60°C in an oven for 30 min, then stirred or blown to mix evenly, and finally the solution present as milky white suspension.\u003cbr\u003e\u003cbr\u003e2. Dewax the slides with 1× Dewaxing solution. Rewarm the dewaxing solution at 60°C for 30 min, immerse the slides into the Dewaxing solution and incubate at 60°C for 30 min.\u003cbr\u003e\u003cbr\u003eNote: Low temperature may affect the effect of dewaxing. Please rewarm the dewaxing solution at 60°C for 30 min before the dewaxing. If the paraffin slides are thick, you can dewax the slides for 50 min.\u003cbr\u003e\u003cbr\u003e3. Wash the slides with tap water for 5 min until froth free.\u003cbr\u003e\u003cbr\u003eNote: Don’t wash the tissue directly, avoid tissue damage.\u003cbr\u003e\u003cbr\u003e4. Absorbs the moisture around the tissue with filter paper for the next step. Or immerse the slides in PBS for use.\u003cbr\u003e\u003cbr\u003e5. For IHC experiment, follow the antigen repair step. For TUNEL or HE staining experiment, please follow the staining kit steps.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at room temperature for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. This product is for research use only.\u003cbr\u003e\u003cbr\u003e2. For your safety and health, please wear lab coats and disposable gloves for operation and follow the procedures of laboratory reagent operation.\u003c\/p\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500mL","offer_id":40305643028538,"sku":"E-CK-A032-500","price":224.95,"currency_code":"USD","in_stock":true},{"title":"200mL","offer_id":40305657970746,"sku":"E-CK-A032-200","price":201.95,"currency_code":"USD","in_stock":true},{"title":"100mL","offer_id":40305658232890,"sku":"E-CK-A032-100","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A032.jpg?v=1710353673"},{"product_id":"annexin-v-biotin-azide-free-lyophilized-powder","title":"Annexin V-Biotin Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Biotin Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Biotin Azide-Free Lyophilized Powder is developed for long-term storage and user-defined concentrations of Biotin-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Biotin - conjugated format of this protein, Annexin V- Biotin, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V- Biotin can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.5847%;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\n\u003cspan\u003eAnnexin V\u003c\/span\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.5847%;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.5847%;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\u003cspan\u003e-20°C\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.5847%;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.5847%;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner of early apoptotic cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/ml. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin V-Biotin have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Biotin. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003c\/li\u003e\n\u003cli\u003eAfter reconstitution, the regent can be stored in the dark under sterile conditions at 2~8°C for at least 6 months.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis product is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor maximal assay performance, this reagent should be used within 12 months. Avoid freeze \/ thaw cycles.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40439135567930,"sku":"E-CK-A110U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40439164567610,"sku":"E-CK-A110U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40439164600378,"sku":"E-CK-A110U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40439164633146,"sku":"E-CK-A110U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A110U.jpg?v=1714023720"},{"product_id":"annexin-v-fitc-azide-free-lyophilized-powder","title":"Annexin V-FITC Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-FITC Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-FITC Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of FITC-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The FITC conjugated format of this protein, Annexin V-FITC, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-FITC can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and PI doublepositive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e490\/530\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin VFITC have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin VFITC. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003cbr\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40439186784314,"sku":"E-CK-A111U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40439217553466,"sku":"E-CK-A111U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40439217586234,"sku":"E-CK-A111U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40439217619002,"sku":"E-CK-A111U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-fitc-azide-free-lyophilized-powder-200-g-elabscience-biotechnology-cell-assays-21920661078074.jpg?v=1752500980"},{"product_id":"annexin-v-elab-fluor-647-azide-free-lyophilized-powder","title":"Annexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® 647 Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of Elab Fluor® 647-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium dependent manner. The Elab Fluor® 647-conjugated format of this protein, Annexin V-Elab Fluor® 647, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-Elab Fluor® 647 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 647 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 647 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e 647\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e650\/670\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin V-Elab Fluor® 647 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Elab Fluor® 647. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003cbr\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40439244750906,"sku":"E-CK-A113U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40439244783674,"sku":"E-CK-A113U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40439244816442,"sku":"E-CK-A113U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40439244849210,"sku":"E-CK-A113U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A113U.jpg?v=1714025387"},{"product_id":"annexin-v-pe-reagent","title":"Annexin V-PE Reagent","description":"\u003ch2\u003eAnnexin V-PE Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-PE Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE, the PE-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE single-positive cells were early apoptotic cells, Annexin VPE and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003ePE\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e495;565\/575\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003ePE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer(E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 5 µL of Annexin V-PE Reagent and 5 µL of DNA dye (7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40439282532410,"sku":"E-CK-A115-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40439282565178,"sku":"E-CK-A115-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40439282597946,"sku":"E-CK-A115-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40439282630714,"sku":"E-CK-A115-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A115.jpg?v=1714026440"},{"product_id":"annexin-v-apc-reagent","title":"Annexin V-APC Reagent","description":"\u003ch2\u003eAnnexin V-APC Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-APC Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for\u003cbr\u003e4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 254.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAPC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e650\/660\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eAPC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 39.2px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 39.2px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer(E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40439367204922,"sku":"E-CK-A117-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40439367237690,"sku":"E-CK-A117-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40439367270458,"sku":"E-CK-A117-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40439367303226,"sku":"E-CK-A117-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A117.jpg?v=1714027746"},{"product_id":"annexin-v-egfp-azide-free-lyophilized-powder","title":"Annexin V-EGFP Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-EGFP Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-EGFP Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of EGFP-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The EGFP conjugated format of this protein, Annexin V-EGFP, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-EGFP can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-EGFP single-positive cells were early apoptotic cells, Annexin V-EGFP and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eEGFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e488\/510\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin VEGFP have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin VEGFP. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40439409475642,"sku":"E-CK-A119U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40439409508410,"sku":"E-CK-A119U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40439409541178,"sku":"E-CK-A119U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40439409573946,"sku":"E-CK-A119U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A119U.jpg?v=1714028527"},{"product_id":"fitc-11-dutp","title":"FITC-11-dUTP","description":"\u003ch2\u003eFITC-11-dUTP\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFITC-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDNA Fragmentation\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eParaffin section, frozen section, cell slide\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFluorescence Microscope\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\"\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e490\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eFilter set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eChromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3 '-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of Terminal Deoxynucleotidyl Transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometry or fluorescence microscope.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A120D-2.jpg?v=1714029780\" width=\"375\" height=\"281\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"250μL","offer_id":40439456792634,"sku":"E-CK-A120D-250","price":457.95,"currency_code":"USD","in_stock":true},{"title":"25μL","offer_id":40439534420026,"sku":"E-CK-A120D-25","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A120D.jpg?v=1714030118"},{"product_id":"elab-fluor-488-11-dutp","title":"Elab Fluor® 488-11-dUTP","description":"\u003ch2\u003eElab Fluor® 488-11-dUTP\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElab Fluor® 488-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDNA Fragmentation\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eParaffin section, frozen section, cell slide\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFluorescence Microscope\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\" data-mce-style=\"width: 67.1944%; height: 22.6625px;\"\u003e\n\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e 488\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\" data-mce-style=\"width: 67.1944%;\"\u003e495\/519\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eFilter set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Elab_Fluor_488-11-dUTP.jpg?v=1714031519\" width=\"355\" height=\"266\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"250μL","offer_id":40439610703930,"sku":"E-CK-A121D-250","price":457.95,"currency_code":"USD","in_stock":true},{"title":"25μL","offer_id":40439610736698,"sku":"E-CK-A121D-25","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A121D.jpg?v=1714031800"},{"product_id":"elab-fluor-594-11-dutp","title":"Elab Fluor® 594-11-dUTP","description":"\u003ch2\u003eElab Fluor® 594-11-dUTP\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElab Fluor® 594-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDNA Fragmentation\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eParaffin section, frozen section, cell slide\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFluorescence Microscope\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\" data-mce-style=\"width: 67.1944%; height: 22.6625px;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e 594\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e590\/617\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eFilter set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eTRITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A122D-Elabscience.jpg?v=1714032081\" width=\"346\" height=\"259\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"250μL","offer_id":40439669293114,"sku":"E-CK-A122D-250","price":457.95,"currency_code":"USD","in_stock":true},{"title":"25μL","offer_id":40439669325882,"sku":"E-CK-A122D-25","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A122D.jpg?v=1714032334"},{"product_id":"annexin-v-pe-cyanine5-reagent","title":"Annexin V-PE\/Cyanine5 Reagent","description":"\u003ch2\u003eAnnexin V-PE\/Cyanine5 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-PE\/Cyanine5 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE\/Cyanine5, the PE\/Cyanine5-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE\/Cyanine5 single-positive cells were early apoptotic cells, Annexin V-PE\/Cyanine5 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 254.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003ePE\/Cyanine5\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e495;565;655\/670\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003ePerCP\/Cy5.5\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 39.2px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 39.2px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.4006%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-PE\/Cyanine5 Reagent and 5 µL of DNA dye (PI [E-CK-A161] or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40439699898426,"sku":"E-CK-A123-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40439699931194,"sku":"E-CK-A123-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40439699963962,"sku":"E-CK-A123-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40439699996730,"sku":"E-CK-A123-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A123.jpg?v=1714033333"},{"product_id":"elab-fluor-647-11-dutp","title":"Elab Fluor® 647-11-dUTP","description":"\u003ch2\u003eElab Fluor® 647-11-dUTP\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElab Fluor® 647-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDNA Fragmentation\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eParaffin section, frozen section, cell slide\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFluorescence Microscope\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\" data-mce-style=\"width: 67.1944%; height: 22.6625px;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e 647\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\u003cspan\u003e650\/665\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eFilter set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eCy5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" height=\"264\" width=\"352\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A124D-Elabscience.jpg?v=1714033710\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"250μL","offer_id":40439779754042,"sku":"E-CK-A124D-250","price":457.95,"currency_code":"USD","in_stock":true},{"title":"25μL","offer_id":40439779786810,"sku":"E-CK-A124D-25","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/elab-fluor-647-11-dutp-250-l-elabscience-biotechnology-cell-assays-21920969162810.jpg?v=1752500770"},{"product_id":"elab-fluor-555-11-dutp","title":"Elab Fluor® 555-11-dUTP","description":"\u003ch2\u003eElab Fluor® 555-11-dUTP\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElab Fluor® 555-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 199.062px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDNA Fragmentation\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eParaffin section, frozen section, cell slide\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eFluorescence Microscope\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.6625px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 22.6625px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\" data-mce-style=\"width: 67.1944%; height: 22.6625px;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e 555\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e555\/565\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eFilter set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eTRITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/Elab_Fluor_555-11-dUTP.jpg?v=1714037920\" width=\"356\" height=\"267\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"250μL","offer_id":40440241487930,"sku":"E-CK-A125D-250","price":457.95,"currency_code":"USD","in_stock":true},{"title":"25μL","offer_id":40440241520698,"sku":"E-CK-A125D-25","price":156.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A125D.jpg?v=1714038322"},{"product_id":"annexin-v-pe-cyanine7-reagent","title":"Annexin V-PE\/Cyanine7 Reagent","description":"\u003ch2\u003eAnnexin V-PE\/Cyanine7 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-PE\/Cyanine7 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE\/Cyanine7, the PE\/Cyanine7-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE\/Cyanine7 single-positive cells were early apoptotic cells, Annexin V-PE\/Cyanine7 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003ePE\/Cyanine7\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e495;565;755\/775\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003ePE\/Cy7\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer(E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-PE\/Cyanine7 Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440287789114,"sku":"E-CK-A127-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440287821882,"sku":"E-CK-A127-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440287854650,"sku":"E-CK-A127-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440287887418,"sku":"E-CK-A127-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A127.jpg?v=1714038837"},{"product_id":"annexin-v-apc-cyanine7-reagent","title":"Annexin V-APC\/Cyanine7 Reagent","description":"\u003ch2\u003eAnnexin V-APC\/Cyanine7 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-APC\/Cyanine7 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC\/Cyanine7, the APC\/Cyanine7-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC\/Cyanine7 single-positive cells were early apoptotic cells, Annexin V-APC\/Cyanine7 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAPC\/Cyanine7\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e650;760\/780\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\" data-mce-style=\"width: 67.1944%;\"\u003eAPC\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC\/Cyanine7 Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440320426042,"sku":"E-CK-A129-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440320458810,"sku":"E-CK-A129-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440320491578,"sku":"E-CK-A129-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440320524346,"sku":"E-CK-A129-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-apc-cyanine7-reagent-500-tests-elabscience-biotechnology-cell-assays-21921678458938.jpg?v=1752500969"},{"product_id":"annexin-v-pe-elab-fluor-594-reagent","title":"Annexin V-PE\/Elab Fluor® 594 Reagent","description":"\u003ch2\u003eAnnexin V-PE\/Elab Fluor® 594 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-PE\/Elab Fluor® 594 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE\/Elab Fluor® 594, the PE\/Elab Fluor® 594-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE\/Elab Fluor® 594 single-positive cells were early apoptotic cells, Annexin V-PE\/Elab Fluor® 594 and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells ere naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003ePE\/Elab Fluor\u003csup\u003e®\u003c\/sup\u003e 594\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e495;565\/620\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eECD\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS(E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003e3. Add 5 µL of Annexin V-PE\/Elab Fluor® 594 Reagent and 5 µL of DNA dye (DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003e4. Gently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003e5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440626511930,"sku":"E-CK-A131-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440626544698,"sku":"E-CK-A131-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440626577466,"sku":"E-CK-A131-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440626610234,"sku":"E-CK-A131-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A131.jpg?v=1714041482"},{"product_id":"annexin-v-elab-fluor-violet-450-azide-free-lyophilized-powder","title":"Annexin V-Elab Fluor® Violet 450 Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 450 Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® Violet 450 Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of Elab Fluor® Violet 450-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium dependent manner. The Elab Fluor® Violet 450-conjugated format of this protein, Annexin V-Elab Fluor® Violet 450, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-Elab Fluor® Violet 450 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 450 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 450 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e\n\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e Violet 450\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e410\/450\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003ePacific Blue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin VElab Fluor® Violet 450 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Elab Fluor® Violet 450. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40440689033274,"sku":"E-CK-A133U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40440689066042,"sku":"E-CK-A133U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40440689098810,"sku":"E-CK-A133U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40440689131578,"sku":"E-CK-A133U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A133U.jpg?v=1714042282"},{"product_id":"annexin-v-elab-fluor-violet-500-azide-free-lyophilized-powder","title":"Annexin V-Elab Fluor® Violet 500 Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 500 Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® Violet 500 Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of Elab Fluor® Violet 500-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium dependent manner. The Elab Fluor® Violet 500-conjugated format of this protein, Annexin V-Elab Fluor® Violet 500, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-Elab Fluor® Violet 500 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 500 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 500 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\" data-mce-style=\"width: 67.1944%;\"\u003e\n\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e Violet 500\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e425\/500\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003ePacific Green\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin VElab Fluor® Violet 500 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Elab Fluor® Violet 500. Otherwise, the lyophilized cake may not be thoroughly soaked in water\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40440727175226,"sku":"E-CK-A135U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40440727207994,"sku":"E-CK-A135U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40440727240762,"sku":"E-CK-A135U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40440727273530,"sku":"E-CK-A135U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A135U.jpg?v=1714042867"},{"product_id":"annexin-v-elab-fluor-488-reagent","title":"Annexin V-Elab Fluor® 488 Reagent","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 488 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® 488 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 488, the Elab Fluor® 488-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 488 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 488 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 156.8px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e488\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e495\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS (E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 488 Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CK-A162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440770592826,"sku":"E-CK-A137-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440770625594,"sku":"E-CK-A137-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440770658362,"sku":"E-CK-A137-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440770691130,"sku":"E-CK-A137-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-488-reagent-500-tests-elabscience-biotechnology-cell-assays-21921755529274.jpg?v=1752500771"},{"product_id":"annexin-v-elab-fluor-488-azide-free-lyophilized-powder","title":"Annexin V-Elab Fluor® 488 Azide-Free Lyophilized Powder","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 488 Azide-Free Lyophilized Powder\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® 488 Azide-Free Lyophilized Powder is developed for long-term storage and user defined concentrations of Elab Fluor® 488-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium dependent manner. The Elab Fluor® 488-conjugated format of this protein, Annexin V-Elab Fluor® 488, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V-Elab Fluor® 488 can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and PI\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 488 single positive cells were early apoptotic cells, Annexin V-Elab Fluor® 488 and PI double-positive cells were necrotic or late apoptotic cells, and PI single positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\" data-mce-style=\"width: 67.1944%;\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e 488\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e495\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eDeionized water (sterile)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e-20°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eReconstitution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg\/mL. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg\/mL Annexin VElab Fluor® 488 have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Elab Fluor® 488. Otherwise, the lyophilized cake may not be thoroughly soaked in water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eThe lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 μg","offer_id":40440772034618,"sku":"E-CK-A137U-200","price":589.95,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":40440772067386,"sku":"E-CK-A137U-100","price":333.95,"currency_code":"USD","in_stock":true},{"title":"50 μg","offer_id":40440772100154,"sku":"E-CK-A137U-50","price":213.95,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":40440772132922,"sku":"E-CK-A137U-25","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-488-azide-free-lyophilized-powder-200-g-elabscience-biotechnology-cell-assays-21921764409402.jpg?v=1752500924"},{"product_id":"annexin-v-elab-fluor-red-780-reagent","title":"Annexin V-Elab Fluor® Red 780 Reagent","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Red 780 Reagent\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V-Elab Fluor® Red 780 Reagent is developed to identify apoptotic cells.\u003c\/p\u003e\n\u003cp\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Red 780, the Elab Fluor® Red 780-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp\u003eCells at different apoptotic stages can be distinguished by using Annexin V and membrane impermeable DNA dyes like Propidium Iodide (PI), 7 7-Amino Actinomycin D (7-AAD) or 4',6-Diamidino-2-Phenylindole (DAPI).\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Red 780 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Red 780 and PI double-positive cells were necrotic or late apoptotic cells,\u003cbr\u003eand PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 257.862px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFluorometric Method\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eCell samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eAssay time\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003e40 min\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 30.9278%; height: 19.6px;\" data-mce-style=\"width: 30.9278%; height: 19.6px;\"\u003e\u003cspan\u003eDetection instrument\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eFlow Cytometer\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.6625px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 22.6625px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eDye Type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 22.6625px;\" data-mce-style=\"width: 67.1944%;\"\u003e\n\u003cspan\u003eElab Fluor\u003c\/span\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e Red 780\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eEx\/Em (nm)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%;\"\u003e\u003cspan\u003e625\/765\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eChannel set\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 68.8513%; height: 19.6px;\" data-mce-style=\"width: 68.8513%; height: 19.6px;\"\u003e\u003cspan\u003eAPC\/Cy7\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 39.2px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eOther reagents required\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 39.2px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V Binding Buffer (E-CK-A151), PBS (E-BC-R187), Deionized water\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C, shading light\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eDetection Principle\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\n\u003cp\u003e\u003cspan\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer [E-CK-A151] to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Red 780 Reagent and 5 µL of DNA dye (PI [E-CK-A161], 7-AAD [E-CKA162]) or DAPI [E-CK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year in the dark. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440773902394,"sku":"E-CK-A139-500","price":738.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440773935162,"sku":"E-CK-A139-200","price":333.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440773967930,"sku":"E-CK-A139-100","price":213.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440774000698,"sku":"E-CK-A139-50","price":123.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-red-780-reagent-500-tests-elabscience-biotechnology-cell-assays-21921775091770.jpg?v=1752500286"},{"product_id":"annexin-v-binding-buffer-10x","title":"Annexin V Binding Buffer (10×)","description":"\u003ch2\u003eAnnexin V Binding Buffer (10×)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Annexin V Binding Buffer (10×) is developed to identify apoptotic and necrotic cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 69.4px;\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003eAnnexin V\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 20.2px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 20.2px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 20.2px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e2~8°C\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 10px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 10px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eInstructions\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAnnexin V Binding Buffer (10×) is a 10×concentrated solution. Dilute with DI water to 1×working solution before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor example: Take 1 mL Annexin V Binding Buffer (10×), dilute with DI water to 10 mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300×g for 5 min and discard the supernatant. Add PBS to the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V- fluorescein and 5 µL of DNA dye (PI[E-CK-A161] or 7-AAD[E-CK-A162] or DAPI[ECK-A163]) to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore at 2~8°C. Valid for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"50 mL","offer_id":40440776917050,"sku":"E-CK-A151-50","price":147.95,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":40440776949818,"sku":"E-CK-A151-10","price":106.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A151.jpg?v=1714046809"},{"product_id":"calcein-am-assay-buffer","title":"Calcein AM Assay Buffer","description":"\u003ch2\u003eCalcein AM Assay Buffer\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Calcein AM Assay Buffer is specifically designed for the loading of Calcein AM probe and is compatible with PI staining working solution. On the basis of cell isotonic buffer, the product increases the efficiency of Calcein AM loading and reduces the leakage of Calcein in anionic cells and P-glycoprotein carrier cells, ensuring the best staining efficiency.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 156.8px;\" width=\"100%\" data-mce-style=\"width: 100%; height: 156.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eShipping\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"width: 32.5847%; height: 19.6px;\" data-mce-style=\"width: 32.5847%; height: 19.6px;\"\u003e\u003cspan\u003eExpiration date\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 67.1944%; height: 19.6px;\" data-mce-style=\"width: 67.1944%; height: 19.6px;\"\u003e\u003cspan\u003e24 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"title\"\u003eDetection Principle\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eElabscience® Calcein AM Assay Buffer is specifically designed for the loading of Calcein AM probe and is compatible with PI staining working solution. On the basis of cell isotonic buffer, the product increases the efficiency of Calcein AM loading and reduces the leakage of Calcein in anionic cells and P-glycoprotein carrier cells, ensuring the best staining efficiency.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSuspended cells: Collect the cells, centrifuge at 300×g for 5 min, discard the supernatant. Add 1 mL of PBS to resuspend the cells, centrifuge at 300×g for 5 min, discard the supernatant. Wash repeatedly 1 time, discard the supernatant.\u003c\/li\u003e\n\u003cli\u003eAdherent cells: Carefully remove the culture medium of adherent cells, add an appropriate amount of PBS to each well to wash cells, repeat wash the slides and remove PBS.\u003c\/li\u003e\n\u003cli\u003eUse Calcein AM Solution(100 μM) [E-CK-A164] and Calcein AM Assay Buffer to prepare an appropriate amount of staining working solution.\u003c\/li\u003e\n\u003cli\u003eSuspended cells: Add 200 μL of Calcein AM staining working solution to resuspend 1~5×105 cells in each group and incubate for 5~15 min at room temperature in the dark.\u003c\/li\u003e\n\u003cli\u003eAdherent cells: Add Calcein AM staining working solution in a ratio of 100 μL per well in a 96-well plate or 200 μL per well in a 24-well plate and incubate at 37°C for 10~30 min.\u003c\/li\u003e\n\u003cli\u003eAfter incubation, the staining effect can be directly detected by flow cytometry or observe under a fluorescence microscope.\u003cbr\u003e\u003cbr\u003eNote 1: Co-staining with Propidium Iodide (PI) Solution(750 μM) [E-CK-A165 ] can distinguish dead cells.\u003cbr\u003e\u003cbr\u003eNote 2: For flow cytometry, Calcein can be detected in FITC channel while PI can be detected in PE or Percp\/Cy5.5 channel.\u003cbr\u003e\u003cbr\u003eNote3: Calcein is green fluorescent, Ex\/Em=494nm\/517nm; PI is red fluorescent, Ex\/Em=535nm\/617nm\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003eStore at -20°C for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003ePlease take safety precautions and follow the procedures of laboratory reagent operation.\u003c\/li\u003e\n\u003cli\u003ePlease store the product at the appropriate temperature to avoid failure.\u003c\/li\u003e\n\u003cli\u003eWash cells with serum-free medium (serum may contain esterase) or PBS before staining. The Buffer should not contain primary or secondary amines, as fatty histamine can lyse AM esters and hinder loading.\u003c\/li\u003e\n\u003cli\u003eExcessive acceleration and deceleration of centrifuge may cause cell loss. It is suggested to adjust the acceleration no more than 3 and deceleration no more than 2, that is, Acc≦3, Dec≦2.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"100 mL","offer_id":40440779079738,"sku":"E-CK-A153-100","price":99.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A153.jpg?v=1714047493"},{"product_id":"pi-reagent-50-g-ml","title":"PI Reagent (50μg\/mL)","description":"\u003ch2\u003ePI Reagent (50μg\/mL)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eElabscience\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e PI Reagent (50μg\/mL) is developed to identify apoptotic and necrotic cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePropidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Propidium Iodide (PI) and Annexin V.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ci\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and Annexin V-FITC.\u003c\/i\u003e\u003c\/span\u003e\u003cspan\u003e\u003ci\u003e\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ci\u003eJurkat cells were cultured with (\u003cb\u003eLeft\u003c\/b\u003e) or without (\u003cb\u003eRight\u003c\/b\u003e) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wsh the cells and resuspend the cells gently followed by the cell counting.\u003cbr\u003e\u003c\/span\u003eNote: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSplit the cell suspension into tubes, 1-5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS\u003c\/span\u003e\u003cspan\u003e to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer \u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_binding_buffer(10%C3%97)[151]-205765.html\" data-mce-href=\"https:\/\/www.elabscience.com\/p-annexin_v_binding_buffer(10%C3%97)[151]-205765.html\" target=\"_blank\"\u003e[E-CK-A151]\u003c\/a\u003e to resuspend the cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 5 µl of Annexin \u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_fitc_reagent[111]-205768.html\" data-mce-href=\"https:\/\/www.elabscience.com\/p-annexin_v_fitc_reagent[111]-205768.html\" target=\"_blank\"\u003eV-FITC[E-CK-A111] \u003c\/a\u003e and 5 µl of PI Reagent (50μg\/mL) to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGently vortex the cells and incubate at room temperature for 15-20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at \u003c\/span\u003e\u003cspan\u003e2~8°C\u003c\/span\u003e\u003cspan\u003e for one year in dark.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor maximal assay performance, this reagent should be used within 12 months. Avoid freeze \/ thaw cycles.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40440780259386,"sku":"E-CK-A161-500","price":62.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40440780292154,"sku":"E-CK-A161-200","price":29.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40440780324922,"sku":"E-CK-A161-100","price":17.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40440780357690,"sku":"E-CK-A161-50","price":12.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/pi-reagent-50-g-ml-500-tests-elabscience-biotechnology-cell-assays-21921791377466.jpg?v=1752500774"},{"product_id":"7-aad-reagent-100-g-ml","title":"7-AAD Reagent (100μg\/mL)","description":"\u003ch2\u003e7-AAD Reagent (100μg\/mL)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eElabscience\u003csup\u003e®\u003c\/sup\u003e\u003c\/span\u003e\u003cspan\u003e 7-AAD Reagent (100μg\/mL) is developed to identify apoptotic and necrotic cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using 7-AAD and Annexin V.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ci\u003e\u003cspan\u003eJurkat cells were treated with 5 μM Camptothecin and detected by this reagent and Annexin V-APC.\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eJurkat cells were treated with 5 \u003c\/span\u003e\u003cspan\u003eμM Camptothecin (\u003cb\u003eRight\u003c\/b\u003e) \u003c\/span\u003e\u003cspan\u003eor not\u003c\/span\u003e\u003cspan\u003e (\u003cb\u003eLeft\u003c\/b\u003e) \u003c\/span\u003e\u003cspan\u003efor 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and 7-AAD double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were nude nuclear cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003cbr\u003e\u003c\/span\u003eNote: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSplit the cell suspension into tubes, 1-5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS\u003c\/span\u003e\u003cspan\u003e to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer\u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_binding_buffer(10%C3%97)[151]-205765.html\" data-mce-href=\"https:\/\/www.elabscience.com\/p-annexin_v_binding_buffer(10%C3%97)[151]-205765.html\" target=\"_blank\"\u003e[E-CK-A151] \u003c\/a\u003eto resuspend the cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 5 µl of \u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_apc_reagent[117]-205757.html\" data-mce-href=\"https:\/\/www.elabscience.com\/p-annexin_v_apc_reagent[117]-205757.html\" target=\"_blank\"\u003eAnnexin V-APC[E-CK-A117]\u003c\/a\u003e and 5 µl of 7-AAD Reagent (100μg\/mL) to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGently vortex the cells and incubate at room temperature for 15-20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at \u003c\/span\u003e\u003cspan\u003e2~8°C\u003c\/span\u003e\u003cspan\u003e for one year in dark.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan\u003eFor maximal assay performance, this reagent should be used within 12 months. Avoid freeze \/ thaw cycles.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40441415073850,"sku":"E-CK-A162-500","price":189.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40441415106618,"sku":"E-CK-A162-200","price":122.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40441415139386,"sku":"E-CK-A162-100","price":99.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40441415172154,"sku":"E-CK-A162-50","price":89.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/7-aad-reagent-100-g-ml-500-tests-elabscience-biotechnology-cell-assays-21922560114746.jpg?v=1752500853"},{"product_id":"dapi-reagent-25-g-ml","title":"DAPI Reagent (25μg\/mL)","description":"\u003ch2\u003eDAPI Reagent (25μg\/mL)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eElabscience\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e  DAPI Reagent (25μg\/mL) is developed to identify apoptotic and necrotic cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"left\" class=\"MsoNormal\"\u003e\u003cspan\u003eDAPI Reagent has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. \u003c\/span\u003e\u003cspan\u003eDue to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using \u003c\/span\u003e\u003cspan\u003eDAPI\u003c\/span\u003e\u003cspan\u003e and Annexin V.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ci\u003e\u003cspan\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this reagent and Annexin V-APC.\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eJurkat cells were cultured with (\u003c\/span\u003e\u003cb\u003e\u003cspan\u003eRight\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e) or without (\u003c\/span\u003e\u003cb\u003e\u003cspan\u003eLeft\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e)  5 μM Camptothecin for 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and DAPI double-positive cells were necrotic or late apoptotic cells, and DAPI single-positive cells were naked nuclei.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"detail_section\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003cbr\u003e\u003c\/span\u003eTip: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSplit the cell suspension into tubes, 1~5 × 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e5\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003csup\u003e \u003c\/sup\u003ecells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer[\u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_binding_buffer(10%C3%97)[151]-205765.html\" target=\"_blank\"\u003eE-CK-A151\u003c\/a\u003e] to resuspend the cells.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAdd 5 µl of Annexin V-APC[\u003ca href=\"https:\/\/www.elabscience.com\/p-annexin_v_apc_reagent[117]-205757.html\" target=\"_blank\"\u003eE-CK-A117\u003c\/a\u003e] and 5 µl of  DAPI Reagent (25μg\/mL) to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at \u003c\/span\u003e\u003cspan\u003e2~8°C\u003c\/span\u003e\u003cspan\u003e for one year in dark.  DAPI Reagent (25μg\/mL) should be spilt into small tubes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp style=\"font-size: 0.875rem; display: inline !important;\" class=\"MsoListParagraph\"\u003eFor maximal assay performance, this reagent should be used within 12 months. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40441418448954,"sku":"E-CK-A163-500","price":127.95,"currency_code":"USD","in_stock":true},{"title":"200 Tests","offer_id":40441418481722,"sku":"E-CK-A163-200","price":94.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40441418514490,"sku":"E-CK-A163-100","price":83.95,"currency_code":"USD","in_stock":true},{"title":"50 Tests","offer_id":40441418547258,"sku":"E-CK-A163-50","price":77.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A163.jpg?v=1714097074"},{"product_id":"calcein-am-solution-100-m","title":"Calcein AM Solution (100 μM)","description":"\u003ch2\u003eCalcein AM Solution (100 μM)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Calcein AM Solution (100 μM) can be used to distinguish living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot pass through the cell membrane, while Calcein can emit strong green fluorescence ( Ex\/Em = 494nm\/517nm ). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. Compared with other live cell staining probes, Calcein AM is less toxic and does not affect cell differentiation and proliferation.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eViability\/Cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e30min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometry, Fluorescence Microscope\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eCalcein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e494\/517\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eFilter set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS (pH7.2~7.6)(E-IR-R187).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReagent Not Supplied\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePBS buffer (pH7.2~7.4).\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cbr\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eExperimental Procedure\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1 Flow cytometry detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1.1 Preparation of working solution\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e1.1.1 Reagent preparation: Take out the frozen Calcein AM Solution (100 μM), after thawing at room temperature, vortex mixing each reagent.\u003c\/li\u003e\n\u003cli\u003e1.1.2 Preparation of Calcein AM staining working solution: After thawing at room temperature, the vortex-mixed Calcein AM Solution and were prepared into the staining working solution at a ratio of 1~5×105 cells\/200 μL. Prepare the staining working solution according the number of samples. Please refer to the table below.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"border-collapse: collapse; width: 412pt; height: 90.6px;\" width=\"549\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\n\u003ccolgroup\u003e\n\u003ccol style=\"width: 268px;\" width=\"269\"\u003e \u003ccol style=\"width: 103px;\" width=\"88\"\u003e \u003ccol style=\"width: 86px;\" width=\"92\"\u003e \u003ccol style=\"width: 90px;\" width=\"100\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 58.8px; width: 202pt;\" width=\"269\" class=\"xl66\" height=\"38\" rowspan=\"2\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 210pt; text-align: center; height: 39.2px;\" width=\"280\" class=\"xl65\" colspan=\"3\"\u003e\u003cstrong\u003eVolume of Calcein AM staining working solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" height=\"19\"\u003e\u003cstrong\u003e1 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e\u003cstrong\u003e5 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e\u003cstrong\u003e10 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 19.6px;\" height=\"19\"\u003eCalcein AM Solution (100 μM)\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e0.1 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e0.5 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e1 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12.2px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 12.2px;\" height=\"19\"\u003ePBS Calcein AM Assay Buffer (E-CK-A153)\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: Calcein AM in the staining working solution is easily deliquescent, prepare the fresh solution before use.\u003c\/p\u003e\n\u003cp\u003eTips: It is suggested that in order to save reagents and ensure the accuracy of the experiment, Calcein AM Solution can be diluted gradiently, such as 100 times to 1 μM with PBS or Calcein AM Assay Buffer. Before staining, use PBS or Calcein AM Assay Buffer to dilute 1μM Calcein AM Solution 100 times to the dyeing concentration (0.01m), that is, 2 μL 1 μM Calcein AM Solution was added to 200 μL PBS or Calcein AM Assay Buffer.\u003c\/p\u003e\n\u003cp\u003e1.2 Staining procedure\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e1.2.1 Collect the cells, centrifuge at 300×g for 5 min, discard the supernatant. Add 1 mL of PBS to resuspend the cells, centrifuge at 300×g for 5 min, discard the supernatant. Wash repeatedly 1 time, discard the supernatant.\u003c\/li\u003e\n\u003cli\u003e1.2.2 Add 200 μL of Calcein AM staining working solution to resuspend 1~5×105 cells in each group and incubate for 5~15 min at room temperature in the dark.\u003c\/li\u003e\n\u003cli\u003e1.2.3 After incubation, flow cytometry can be performed directly. If it cannot be detected in time, it is recommended to avoid light and place in a 4°C refrigerator for detection within 2 hours.\u003cbr\u003eNote: Calcein can be detected in FITC channel. Propidium iodide (PI) solution (750 μM) (E-CKA165) can be selected when it is necessary to distinguish dead cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e2 Fluorescence microscope detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2.1 Preparation of working solution\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e2.1.1 Reagent preparation: Take out the frozen Calcein AM Solution (100 μM), after thawing at room temperature, vortex mixing each reagent.\u003c\/li\u003e\n\u003cli\u003e2.1.2 Preparation of Calcein AM staining working solution: After thawing at room temperature, the vortex-mixed Calcein AM Solution were prepared into the staining working solution according to 100 μL per well of 96-well plate or 200 μL per well in a 24-well plate. Prepare the staining working solution according the number of samples. Please refer to the table below.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"border-collapse: collapse; width: 412pt; height: 89.6px;\" width=\"549\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 58.8px; width: 202pt;\" width=\"269\" class=\"xl66\" height=\"38\" rowspan=\"2\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 210pt; text-align: center; height: 39.2px;\" width=\"280\" class=\"xl65\" colspan=\"3\"\u003e\u003cstrong\u003eVolume of Calcein AM staining working solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" height=\"19\"\u003e\u003cstrong\u003e1 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e\u003cstrong\u003e5 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e\u003cstrong\u003e10 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 19.6px;\" height=\"19\"\u003eCalcein AM Solution (10 μM)\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e10 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 11.2px;\" height=\"19\"\u003e\n\u003ctd style=\"height: 11.2px;\" height=\"19\"\u003ePBS Calcein AM Assay Buffer (E-CK-A153)\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: Calcein AM in the staining working solution is easily deliquescent, prepare the fresh solution before use.\u003c\/p\u003e\n\u003cp\u003eCalcein AM Assay Buffer is conducive to the loading of fluorescent probes and the maintenance of fluorescent signals. If adherent cells are easy to fall off and sensitive, it is recommended to use basic medium to prepare the above staining working solution.\u003c\/p\u003e\n\u003cp\u003e2.2 Staining process\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e2.2.1 Carefully remove the culture medium of adherent cells, add an appropriate amount of PBS to each well to wash cells, repeat wash the slides and remove PBS.\u003c\/li\u003e\n\u003cli\u003e2.2.2 Add Calcein AM staining working solution in a ratio of 100 μL per well in a 96-well plate or 200 μL per well in a 24-well plate and incubate at 37°C for 10~30 min. (It is necessary to extend the dyeing time to 30~60min when the basic medium is used to prepare the dyeing working solution. If it is necessary to distinguish dead cells at the same time, PI solution can be added 10 min before the end of the reaction.)\u003c\/li\u003e\n\u003cli\u003e2.2.3 Observe under fluorescence microscope after incubation (Calcein is green fluorescent, Ex\/Em=494nm\/517nm).\u003cbr\u003eNote 1: For suspended cells, after collecting cell precipitation, add Calcein AM staining working solution at a ratio of 1~5×105 cells\/200 μL and incubate at room temperature for 15~20 min. Add the cell suspension to the glass slide, cover the cover glass gently, and then observe under the microscope.\u003cbr\u003eNote 2: Propidium iodide (PI) solution (750 μM) (E-CK-A165) can be selected when it is necessary \u003cspan style=\"font-size: 0.875rem;\"\u003eto distinguish dead cells. PI is red fluorescent, Ex\/Em=535nm\/617nm.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore protected from light at -20°C for 12 months. It is recommend that Calcein AM Solution (100 μM) be properly packaged and stored protected from light for the first time to prevent spontaneous ester hydrolysis in a damp environment.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThis kit is for research use only.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePlease take safety precautions and follow the procedures of laboratory reagent operation.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003ePlease store the product at the appropriate temperature to avoid failure.\u003c\/li\u003e\n\u003cli\u003eWash cells with serum-free medium (serum may contain esterase) or PBS before staining. The Buffer should not contain primary or secondary amines, as fatty histamine can lyse AM esters and hinder loading.\u003c\/li\u003e\n\u003cli\u003eThe staining temperature of 37°C can reduce the staining time. Staining at room temperature can reduce the slide effect of fluorescent probe penetration into organelles.\u003c\/li\u003e\n\u003cli\u003eMn2+ has fluorescence quenching effect, so pay attention not to contain metal ions such as Mn2+ in the washing buffer.\u003c\/li\u003e\n\u003cli\u003eIt is suitable for any animal cells containing esterase activity. Calcein AM is not suitable for plants and bacteria because it cannot enter the cell wall.\u003c\/li\u003e\n\u003cli\u003eExcessive acceleration and deceleration of centrifuge may cause cell loss. It is suggested to adjust the acceleration no more than 3 and deceleration no more than 2, that is, Acc≦3, Dec≦2.\u003c\/li\u003e\n\u003cli\u003eWhen using fluorescence microscope for in situ detection of cells with weak adhesion ability, cell culture vessels can be subjected to anti-detachment treatment before cell inoculation and staining. The PI staining time should be less than 30 min, otherwise it may lead to false positive of PI. If you want to extend the staining time of Calcein AM, PI can be added within 10-30 min before the end of Calcein AM staining, and observe and take photos within 1-2 hours.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40441427132474,"sku":"E-CK-A164-500","price":292.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40441427198010,"sku":"E-CK-A164-100","price":101.95,"currency_code":"USD","in_stock":true},{"title":"500 Tests × 10","offer_id":40441427230778,"sku":"E-CK-A164-500-10","price":782.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A164.jpg?v=1714098825"},{"product_id":"propidium-iodide-pi-solution-750-m","title":"Propidium Iodide (PI) Solution (750 μM)","description":"\u003ch2\u003ePropidium Iodide (PI) Solution (750 μM)\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePI is a kind of nucleic acid dye. Due to the loss of cell membrane selective permeability of dead cells, Propidium Iodide (PI) can enter the cell and specifically bind with double-stranded DNA and produce red fluorescence (Ex\/Em=535nm\/617nm), thus marking dead cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eViability\/Cytotoxicity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e30min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometry, Fluorescence Microscope\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003ePI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e535\/617\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePE, PerCP\/Cy5.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eFilter set\u003c\/td\u003e\n\u003ctd\u003eTRITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS (pH7.2~7.6)(E-IR-R187).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eExperimental Procedure\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1 Flow cytometry detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1.1 Preparation of working solution\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e1.1.1 Reagent preparation: Take out the frozen PI Solution (750 μM), after thawing at room temperature, vortex mixing each reagent.\u003c\/li\u003e\n\u003cli\u003e1.1.2 Preparation of PI staining working solution: After thawing at room temperature, the vortex-mixed PI solution were prepared into the staining working solution at a ratio of 1~5×105 cells\/200 μL. Prepare the staining working solution according the number of samples. Please refer to the table below.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"border-collapse: collapse; width: 412pt; height: 90.6px;\" width=\"549\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\" data-mce-style=\"border-collapse: collapse; width: 412pt; height: 90.6px;\"\u003e\n\u003ccolgroup\u003e \u003ccol style=\"width: 268px;\" width=\"269\" data-mce-style=\"width: 268px;\"\u003e \u003ccol style=\"width: 103px;\" width=\"88\" data-mce-style=\"width: 103px;\"\u003e \u003ccol style=\"width: 86px;\" width=\"92\" data-mce-style=\"width: 86px;\"\u003e \u003ccol style=\"width: 90px;\" width=\"100\" data-mce-style=\"width: 90px;\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\" height=\"19\" data-mce-style=\"height: 39.2px;\"\u003e\n\u003ctd style=\"height: 58.8px; width: 202pt;\" width=\"269\" class=\"xl66\" height=\"38\" rowspan=\"2\" data-mce-style=\"height: 58.8px; width: 202pt;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 210pt; text-align: center; height: 39.2px;\" width=\"280\" class=\"xl65\" colspan=\"3\" data-mce-style=\"width: 210pt; text-align: center; height: 39.2px;\"\u003e\u003cstrong\u003ePI staining working solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e1 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e5 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e10 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003ePI Solution (750 μM) \u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e1 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e5 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 12.2px;\" height=\"19\" data-mce-style=\"height: 12.2px;\"\u003e\n\u003ctd style=\"height: 12.2px;\" height=\"19\" data-mce-style=\"height: 12.2px;\"\u003ePBS\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\" data-mce-style=\"height: 12.2px;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\" data-mce-style=\"height: 12.2px;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"height: 12.2px;\" class=\"xl65\" data-mce-style=\"height: 12.2px;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e1.2.1 Collect the cells, centrifuge at 300×g for 5 min, discard the supernatant. Add 1 mL of PBS to resuspend the cells, centrifuge at 300×g for 5 min, discard the supernatant. Wash repeatedly 1 time, discard the supernatant.\u003c\/li\u003e\n\u003cli\u003e1.2.2 Add 200 μL of PI staining working solution to resuspend 1~5×105 cells in each group and incubate for 5~15 min at room temperature in the dark.\u003c\/li\u003e\n\u003cli\u003e1.2.3 After incubation, flow cytometry can be performed directly. If it cannot be detected in time, it is recommended to avoid light and place in a 4°C refrigerator for detection within 2 hours.\u003cbr\u003eNote: PI can be detected in PE or Percp\/Cy5.5 channel. Calcein AM Solution (100 μM) (E-CKA164) can be selected for co-staining when it is necessary to distinguish living cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2 Fluorescence microscope detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2.1 Preparation of working solution\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e2.1.1 Reagent preparation: Take out the frozen PI Solution (750 μM), after thawing at room temperature, vortex mixing each reagent.\u003c\/li\u003e\n\u003cli\u003e2.1.2 Preparation of PI staining working solution: After thawing at room temperature, the vortex-mixed PI solution were prepared into the staining working solution according to 100 μL per well of 96-well plate or 200 μL per well in a 24-well plate. Prepare the staining working solution according the number of samples. Please refer to the table below.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"border-collapse: collapse; width: 412pt; height: 89.6px;\" width=\"549\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\" data-mce-style=\"border-collapse: collapse; width: 412pt; height: 89.6px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\" height=\"19\" data-mce-style=\"height: 39.2px;\"\u003e\n\u003ctd style=\"height: 58.8px; width: 202pt;\" width=\"269\" class=\"xl66\" height=\"38\" rowspan=\"2\" data-mce-style=\"height: 58.8px; width: 202pt;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 210pt; text-align: center; height: 39.2px;\" width=\"280\" class=\"xl65\" colspan=\"3\" data-mce-style=\"width: 210pt; text-align: center; height: 39.2px;\"\u003e\u003cstrong\u003eVolume of PI staining working solution\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e1 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e5 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e\u003cstrong\u003e10 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\" height=\"19\" data-mce-style=\"height: 19.6px;\"\u003ePI Solution (750 μM)\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e10 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\" class=\"xl65\" data-mce-style=\"height: 19.6px;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 11.2px;\" height=\"19\" data-mce-style=\"height: 11.2px;\"\u003e\n\u003ctd style=\"height: 11.2px;\" height=\"19\" data-mce-style=\"height: 11.2px;\"\u003ePBS\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\" data-mce-style=\"height: 11.2px;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\" data-mce-style=\"height: 11.2px;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"height: 11.2px;\" class=\"xl65\" data-mce-style=\"height: 11.2px;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e2.2 Staining process\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e2.2.1 Carefully remove the culture medium of adherent cells, add an appropriate amount of PBS to each well to wash cells, repeat wash the slides and remove PBS.\u003c\/li\u003e\n\u003cli\u003e2.2.2 Add PI staining working solution in a ratio of 100 μL per well in a 96-well plate or 200 μL per well in a 24-well plate and incubate at 37°C for 10~30 min.\u003c\/li\u003e\n\u003cli\u003e2.2.3 Observe under fluorescence microscope after incubation (Calcein is green fluorescent, Ex\/Em=494nm\/517nm; PI is red fluorescent, Ex\/Em=535nm\/617nm).\u003cbr\u003eNote1: For suspended cells, after collecting cell precipitation, add Calcein AM\/PI staining working solution at a ratio of 1~5×105 cells\/200 μL and incubate at room temperature for 15~20 min. Add the cell suspension to the glass slide, cover the cover glass gently, and then observe under the microscope.\u003cbr\u003eNote 2: Calcein AM Solution (100 μM) (E-CK-A164) can be selected for co-staining when it is necessary to distinguish living cells (Calcein is green fluorescent, Ex\/Em=494nm\/517nm).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -20°C for 12 months. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThis kit is for research use only.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePlease take safety precautions and follow the procedures of laboratory reagent operation.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePlease store the product at the appropriate temperature to avoid failure.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eMn2+ has fluorescence quenching effect, so pay attention not to contain metal ions such as Mn2+ in the washing buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eExcessive acceleration and deceleration of centrifuge may cause cell loss. It is suggested to adjust the acceleration no more than 3 and deceleration no more than 2, that is, Acc≦3, Dec≦2.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"500 Tests","offer_id":40441440174138,"sku":"E-CK-A165-500","price":167.95,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":40441440206906,"sku":"E-CK-A165-100","price":99.95,"currency_code":"USD","in_stock":true},{"title":"500 Tests × 10","offer_id":40441440239674,"sku":"E-CK-A165-500-10","price":336.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/propidium-iodide-pi-solution-750-m-500-tests-elabscience-biotechnology-cell-assays-21922639937594.jpg?v=1752500963"},{"product_id":"annexin-v-fitc-pi-apoptosis-kit","title":"Annexin V-FITC\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-FITC\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-FITC\/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e490\/530\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAdd 5 µL of Annexin V-FITC and 5 µL of PI to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003e\u003c\/span\u003eNote: Annexin V-FITC can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to ECD channel for PI detection.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-FITC and 2.5 µL of PI to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-FITC can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to ECD channel \u003cspan style=\"font-size: 0.875rem;\"\u003efor PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441473794106,"sku":"E-CK-A211-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441478709306,"sku":"E-CK-A211-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441478742074,"sku":"E-CK-A211-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441478774842,"sku":"E-CK-A211-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-fitc-pi-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922693414970.jpg?v=1752501072"},{"product_id":"annexin-v-fitc-7-aad-apoptosis-kit","title":"Annexin V-FITC\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-FITC\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-FITC\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e490\/530\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-FITC and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-FITC can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-FITC and 2.5 µL of 7-AAD to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-FITC can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441479331898,"sku":"E-CK-A212-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441479364666,"sku":"E-CK-A212-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441479397434,"sku":"E-CK-A212-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441479430202,"sku":"E-CK-A212-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-fitc-7-aad-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922700329018.jpg?v=1752500288"},{"product_id":"annexin-v-elab-fluor-647-pi-apoptosis-kit","title":"Annexin V-Elab Fluor® 647\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 647\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® 647 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 647, the Elab Fluor® 647-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 647 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 647 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e647\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/670\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.  \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 647 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 647 can be detected in APC channel while ECD channel is preferred to PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected \u003cspan style=\"font-size: 0.875rem;\"\u003efor PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® 647 and 2.5 µL of PI to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 647 can be detected in APC channel while ECD channel is preferred to PE channel \u003cspan style=\"font-size: 0.875rem;\"\u003efor PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003efor PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441480446010,"sku":"E-CK-A213-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441480478778,"sku":"E-CK-A213-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441480511546,"sku":"E-CK-A213-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441480544314,"sku":"E-CK-A213-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A213.jpg?v=1714105387"},{"product_id":"annexin-v-elab-fluor-647-7-aad-apoptosis-kit","title":"Annexin V-Elab Fluor® 647\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 647\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® 647 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 647, the Elab Fluor® 647-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 647 singlepositive cells were early apoptotic cells, Annexin V-Elab Fluor® 647 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-\u003cbr\u003eAAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e647\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/670\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.   \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 647 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 647 can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® 647 and 2.5 µL of 7-AAD to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 647 can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441481789498,"sku":"E-CK-A214-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441481822266,"sku":"E-CK-A214-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441481855034,"sku":"E-CK-A214-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441481887802,"sku":"E-CK-A214-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A214.jpg?v=1714106022"},{"product_id":"annexin-v-pe-7-aad-apoptosis-kit","title":"Annexin V-PE\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-PE\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-PE\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE, the PE-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE single-positive cells were early apoptotic cells, Annexin VPE and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003ePE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e495;565\/575\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.    \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-PE and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-PE can be detected in PE channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cell  and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-PE and 2.5 µL of 7-AAD to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-PE can be detected in PE channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441492144186,"sku":"E-CK-A216-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441492176954,"sku":"E-CK-A216-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441492209722,"sku":"E-CK-A216-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441492242490,"sku":"E-CK-A216-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A216.jpg?v=1714107055"},{"product_id":"annexin-v-apc-pi-apoptosis-kit","title":"Annexin V-APC\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-APC\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-APC\/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/660\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC can be detected in APC channel while ECD channel is preferred to PE channel for PI \u003cspan style=\"font-size: 0.875rem;\"\u003edetection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected for \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-APC and 2.5 µL of PI to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC can be detected in APC channel while ECD channel is preferred to PE channel for PI \u003cspan style=\"font-size: 0.875rem;\"\u003edetection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected for \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441494929466,"sku":"E-CK-A217-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441494962234,"sku":"E-CK-A217-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441494995002,"sku":"E-CK-A217-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441495027770,"sku":"E-CK-A217-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A217.jpg?v=1714107833"},{"product_id":"annexin-v-apc-7-aad-apoptosis-kit","title":"Annexin V-APC\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-APC\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-APC\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/660\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-APC and 2.5 µL of 7-AAD to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441498435642,"sku":"E-CK-A218-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441498468410,"sku":"E-CK-A218-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441498501178,"sku":"E-CK-A218-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441498533946,"sku":"E-CK-A218-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A218.jpg?v=1714109156"},{"product_id":"annexin-v-egfp-pi-apoptosis-kit","title":"Annexin V-EGFP\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-EGFP\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-EGFP\/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-EGFP, the EGFP-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-EGFP single-positive cells were early apoptotic cells, Annexin V-EGFP and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eEGFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e488\/510\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-EGFP and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-EGFP can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to ECD \u003cspan style=\"font-size: 0.875rem;\"\u003echannel for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-EGFP and 2.5 µL of PI to each tube. \u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-EGFP can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to ECD \u003cspan style=\"font-size: 0.875rem;\"\u003echannel for PI detection\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThis kit is for research use only.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441504661562,"sku":"E-CK-A219-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441504694330,"sku":"E-CK-A219-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441504727098,"sku":"E-CK-A219-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441504759866,"sku":"E-CK-A219-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-egfp-pi-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922752593978.jpg?v=1752500956"},{"product_id":"annexin-v-egfp-7-aad-apoptosis-kit","title":"Annexin V-EGFP\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-EGFP\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-EGFP\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-EGFP, the EGFP-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-EGFP single-positive cells were early apoptotic cells, Annexin V-EGFP and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eEGFP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e488\/510\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-EGFP and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-EGFP can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-EGFP and 2.5 µL of 7-AAD to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-EGFP can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441518096442,"sku":"E-CK-A220-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441518129210,"sku":"E-CK-A220-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441518161978,"sku":"E-CK-A220-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441518194746,"sku":"E-CK-A220-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A220.jpg?v=1714111751"},{"product_id":"annexin-v-cyanine5-pi-apoptosis-kit","title":"Annexin V-Cyanine5\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Cyanine5\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Cyanine5\/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Cyanine5, the Cyanine5-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Cyanine5 single-positive cells were early apoptotic cells, Annexin V-Cyanine5 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eCyanine5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/670\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Cyanine5 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Cyanine5 can be detected in APC channel while ECD channel is preferred to PE channel for \u003cspan style=\"font-size: 0.875rem;\"\u003ePI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Cyanine5 and 2.5 µL of PI to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Cyanine5 can be detected in APC channel while ECD channel is preferred to PE channel for \u003cspan style=\"font-size: 0.875rem;\"\u003ePI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441560793146,"sku":"E-CK-A221-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441560825914,"sku":"E-CK-A221-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441560858682,"sku":"E-CK-A221-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441560891450,"sku":"E-CK-A221-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A221.jpg?v=1714116598"},{"product_id":"annexin-v-cyanine5-7-aad-apoptosis-kit","title":"Annexin V-Cyanine5\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Cyanine5\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Cyanine5\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Cyanine5, the Cyanine5-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Cyanine5 single-positive cells were early apoptotic cells, Annexin V-Cyanine5 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD -single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eCyanine5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650\/670\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Cyanine5 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Cyanine5 can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Cyanine5 and 2.5 µL of 7-AAD to each tube. \u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Cyanine5 can be detected in APC channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441583960122,"sku":"E-CK-A222-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441583992890,"sku":"E-CK-A222-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441584025658,"sku":"E-CK-A222-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441584058426,"sku":"E-CK-A222-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A222.jpg?v=1714117138"},{"product_id":"annexin-v-pe-cyanine7-7-aad-apoptosis-kit","title":"Annexin V-PE\/Cyanine7\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-PE\/Cyanine7\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-PE\/Cyanine7\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-PE\/Cyanine7, the PE\/Cyanine7-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-PE\/Cyanine7 single-positive cells were early apoptotic cells, Annexin V-PE\/Cyanine7 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003ePE\/Cyanine7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e495;565;755\/775\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePE\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAdd 5 µL of Annexin V-PE\/Cyanine7 and 5 µL of 7-AAD to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003e\u003c\/span\u003eNote: Annexin V-PE\/Cyanine7 can be detected in PE\/Cy7 channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-PE\/Cyanine7 and 2.5 µL of 7-AAD to each tube\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-PE\/Cyanine7 can be detected in PE\/Cy7 channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441595232314,"sku":"E-CK-A228-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441595265082,"sku":"E-CK-A228-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441595297850,"sku":"E-CK-A228-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441595330618,"sku":"E-CK-A228-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A228.jpg?v=1714117722"},{"product_id":"annexin-v-apc-cyanine7-pi-apoptosis-kit","title":"Annexin V-APC\/Cyanine7\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-APC\/Cyanine7\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-APC\/Cyanine7\/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC\/Cyanine7, the APC\/Cyanine7-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC\/Cyanine7 single-positive cells were early apoptotic cells, Annexin V-APC\/Cyanine7 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cyanine7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650;760\/780\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC\/Cyanine7 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC\/Cyanine7 can be detected in APC\/Cy7 channel while ECD channel is preferred to PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected for PI detection.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-APC\/Cyanine7 and 2.5 µL of PI to each tube.\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC\/Cyanine7 can be detected in APC\/Cy7 channel while ECD channel is preferred to PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel is selected for PI detection.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441619054650,"sku":"E-CK-A229-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441619087418,"sku":"E-CK-A229-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441619120186,"sku":"E-CK-A229-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441619152954,"sku":"E-CK-A229-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A229.jpg?v=1714118445"},{"product_id":"annexin-v-apc-cyanine7-7-aad-apoptosis-kit","title":"Annexin V-APC\/Cyanine7\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-APC\/Cyanine7\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-APC\/Cyanine7\/7-AAD Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC\/Cyanine7, the APC\/Cyanine7-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC\/Cyanine7 single-positive cells were early apoptotic cells, Annexin V-APC\/Cyanine7 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cyanine7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e650;760\/780\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-APC\/Cyanine7 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC\/Cyanine7 can be detected in APC\/Cy7 channel while 7-AAD can be detected in \u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant.  Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-APC\/Cyanine7 and 2.5 µL of 7-AAD to each tube\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-APC\/Cyanine7 can be detected in APC\/Cy7 channel while 7-AAD can be detected in \u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441639731258,"sku":"E-CK-A230-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441639764026,"sku":"E-CK-A230-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441639796794,"sku":"E-CK-A230-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441639829562,"sku":"E-CK-A230-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-apc-cyanine7-7-aad-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922883895354.jpg?v=1752500782"},{"product_id":"annexin-v-elab-fluor-violet-450-pi-apoptosis-kit","title":"Annexin V-Elab Fluor® Violet 450\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 450\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Violet 450 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Violet 450, the Elab Fluor® Violet 450-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 450 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 450 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eViolet 450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e410\/450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePacific Blue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized  water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Violet 450 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 450 can be detected in Pacific Blue channel while ECD channel is preferred \u003cspan style=\"font-size: 0.875rem;\"\u003eto PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003echannel is selected for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Violet 450 and 2.5 µL of PI to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 450 can be detected in Pacific Blue channel while ECD channel is preferred \u003cspan style=\"font-size: 0.875rem;\"\u003eto PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003echannel is selected for PI detection\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441641041978,"sku":"E-CK-A233-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441641074746,"sku":"E-CK-A233-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441641107514,"sku":"E-CK-A233-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441641140282,"sku":"E-CK-A233-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-violet-450-pi-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922892021818.jpg?v=1752500923"},{"product_id":"annexin-v-elab-fluor-violet-450-7-aad-apoptosis-kit","title":"Annexin V-Elab Fluor® Violet 450\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 450\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Violet 450 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Violet 450, the Elab Fluor® Violet 450-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 450 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 450 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eViolet 450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e410\/450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePacific Blue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.  \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAdd 5 µL of Annexin V-Elab Fluor® Violet 450 and 5 µL of 7-AAD to each tube.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003e\u003c\/span\u003eNote: Annexin V-Elab Fluor® Violet 450 can be detected in Pacific Blue channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Violet 450 and 2.5 µL of 7-AAD to each tube.(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 450 can be detected in Pacific Blue channel while 7-AAD can be detected \u003cspan style=\"font-size: 0.875rem;\"\u003ein PerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441651036218,"sku":"E-CK-A234-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441651068986,"sku":"E-CK-A234-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441651101754,"sku":"E-CK-A234-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441651134522,"sku":"E-CK-A234-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A234.jpg?v=1714122208"},{"product_id":"annexin-v-elab-fluor-violet-500-pi-apoptosis-kit","title":"Annexin V-Elab Fluor® Violet 500\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 500\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Violet 500 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Violet 500, the Elab Fluor® Violet 500-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003cbr\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 500 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 500 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eViolet 500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e425\/500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePacific Green\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Violet 500 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 500 can be detected in Pacific Green channel while ECD channel is \u003cspan style=\"font-size: 0.875rem;\"\u003epreferred to PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel is selected for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Violet 500 and 2.5 µL of PI to each tube\u003cbr\u003e(Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 500 can be detected in Pacific Green channel while ECD channel is \u003cspan style=\"font-size: 0.875rem;\"\u003epreferred to PE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel is selected for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441652510778,"sku":"E-CK-A235-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441652543546,"sku":"E-CK-A235-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441652576314,"sku":"E-CK-A235-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441652609082,"sku":"E-CK-A235-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A235.jpg?v=1714122904"},{"product_id":"annexin-v-elab-fluor-violet-500-7-aad-apoptosis-kit","title":"Annexin V-Elab Fluor® Violet 500\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Violet 500\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Violet 500 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Violet 500, the Elab Fluor® Violet 500-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Violet 500 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Violet 500 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eViolet 500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e425\/500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003ePacific Green\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Violet 500 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 500 can be detected in Pacific Green channel while 7-AAD can be detected \u003cspan style=\"font-size: 0.875rem;\"\u003ein PerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Violet 500 and 2.5 µL of 7-AAD to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Violet 500 can be detected in Pacific Green channel while 7-AAD can be detected \u003cspan style=\"font-size: 0.875rem;\"\u003ein PerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441653493818,"sku":"E-CK-A236-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441653526586,"sku":"E-CK-A236-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441653559354,"sku":"E-CK-A236-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441653592122,"sku":"E-CK-A236-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A236.jpg?v=1714123475"},{"product_id":"annexin-v-elab-fluor-488-pi-apoptosis-kit","title":"Annexin V-Elab Fluor® 488\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 488\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® 488 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 488, the Elab Fluor® 488-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 488 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® 488 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e488\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e495\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 488 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 488 can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to \u003cspan style=\"font-size: 0.875rem;\"\u003eECD channel for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® 488 and 2.5 µL of PI to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 488 can be detected in FITC channel while PerCP\/Cy5.5 channel is preferred to \u003cspan style=\"font-size: 0.875rem;\"\u003eECD channel for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441660571706,"sku":"E-CK-A237-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441660604474,"sku":"E-CK-A237-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441660637242,"sku":"E-CK-A237-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441660670010,"sku":"E-CK-A237-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A237-2.jpg?v=1714125682"},{"product_id":"annexin-v-elab-fluor-488-7-aad-apoptosis-kit","title":"Annexin V-Elab Fluor® 488\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® 488\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® 488 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® 488, the Elab Fluor® 488-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® 488 singlepositive cells were early apoptotic cells, Annexin V-Elab Fluor® 488 and 7-AAD double-positive cells were necrotic or late apoptotic cells, and 7-\u003cbr\u003eAAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e488\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e495\/520\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eFITC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® 488 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 488 can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 channel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® 488 and 2.5 µL of 7-AAD to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® 488 can be detected in FITC channel while 7-AAD can be detected in PerCP\/Cy5.5 \u003cspan style=\"font-size: 0.875rem;\"\u003echannel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441665978426,"sku":"E-CK-A238-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441666011194,"sku":"E-CK-A238-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441666043962,"sku":"E-CK-A238-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441666076730,"sku":"E-CK-A238-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A238.jpg?v=1714125563"},{"product_id":"annexin-v-elab-fluor-red-780-pi-apoptosis-kit","title":"Annexin V-Elab Fluor® Red 780\/PI Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Red 780\/PI Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Red 780 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Red 780, the Elab Fluor® Red 780-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Red 780 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Red 780 and PI double-positive cells were necrotic or late apoptotic cells,\u003cbr\u003eand PI single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eRed 780\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e625\/765\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Red 780 and 5 µL of PI to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Red 780 can be detected in APC\/Cy7 channel while ECD channel is preferred to \u003cspan style=\"font-size: 0.875rem;\"\u003ePE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eis selected for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Red 780 and 2.5 µL of PI to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Red 780 can be detected in APC\/Cy7 channel while ECD channel is preferred to \u003cspan style=\"font-size: 0.875rem;\"\u003ePE channel for PI detection; if the sample has the autofluorescence of the FITC channel, the PerCP\/Cy5.5 channel \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eis selected for PI detection.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441671057466,"sku":"E-CK-A239-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441671090234,"sku":"E-CK-A239-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441671123002,"sku":"E-CK-A239-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441671155770,"sku":"E-CK-A239-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/annexin-v-elab-fluor-red-780-pi-apoptosis-kit-200-assays-elabscience-biotechnology-cell-assays-21922927116346.jpg?v=1752500960"},{"product_id":"annexin-v-elab-fluor-red-780-7-aad-apoptosis-kit","title":"Annexin V-Elab Fluor® Red 780\/7-AAD Apoptosis Kit","description":"\u003ch2\u003eAnnexin V-Elab Fluor® Red 780\/7-AAD Apoptosis Kit\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eElabscience® Annexin V-Elab Fluor® Red 780 Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAnnexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Elab Fluor® Red 780, the Elab Fluor® Red 780- conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDue to the loss of integrity of membrane, 7-AAD can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and 7-AAD.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cem\u003eJurkat cells were treated with 5 μM Camptothecin and detected with this kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eJurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-Elab Fluor® Red 780 single-positive cells were early apoptotic cells, Annexin V-Elab Fluor® Red 780 and 7-AAD double-positive cells were necrotic or late apoptotic\u003cbr\u003ecells, and 7-AAD single-positive cells were naked nuclei.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cb\u003eProperties\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"dtl_table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eAnnexin V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection method\u003c\/td\u003e\n\u003ctd\u003eFluorometric Method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eAssay time\u003c\/td\u003e\n\u003ctd\u003e40min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDetection instrument\u003c\/td\u003e\n\u003ctd\u003eFlow Cytometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eDye Type\u003c\/td\u003e\n\u003ctd\u003eElab Fluor\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eRed 780\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eEx\/Em (nm)\u003c\/td\u003e\n\u003ctd\u003e625\/765\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eChannel set\u003c\/td\u003e\n\u003ctd\u003eAPC\/Cy7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eOther reagents required\u003c\/td\u003e\n\u003ctd\u003ePBS(E-BC-R187), Deionized water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2~8°C, shading light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eIce bag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"30%\"\u003eExpiration date\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e1×Annexin V Binding Buffer: Dilute Annexin V Binding Buffer (10×) with deionized water to 1×Annexin V Binding Buffer before use. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eStaining Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003eControl settings for Annexin V experiments are critical to experimental results. It is strongly recommended to read this article before the experiment: https:\/\/www.elabscience.com\/resource-cell_function-detail-984\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 5 µL of Annexin V-Elab Fluor® Red 780 and 5 µL of 7-AAD to each tube.\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Red 780 can be detected in APC\/Cy7 channel while 7-AAD can be detected in \u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTwo-step process\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003col\u003e\n\u003cli\u003eInduce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and resuspend the cells gently followed by the cell counting.\u003c\/li\u003e\n\u003cli\u003eSplit the cell suspension into tubes, 1~5×105 cells for each, centrifuge at 300 ×g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 100 μL of 1×Annexin V Binding Buffer to resuspend the cells.\u003c\/li\u003e\n\u003cli\u003eAdd 2.5 µL of Annexin V-Elab Fluor® Red 780 and 2.5 µL of 7-AAD to each tube. (Attributed to the higher resolution of two-step protocol, half the amount of the reagents can still guarantee a result of matched quality as in the one-step protocol. It's also recommended that users titrate the reagents for optimal performance in specific models.)\u003c\/li\u003e\n\u003cli\u003eGently vortex the cells and incubate at room temperature for 15~20 min in the dark.\u003c\/li\u003e\n\u003cli\u003eAdd 400 μL of 1×Annexin V Binding Buffer to the tube, and mix gently.\u003c\/li\u003e\n\u003cli\u003eAnalyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.\u003cbr\u003eNote: Annexin V-Elab Fluor® Red 780 can be detected in APC\/Cy7 channel while 7-AAD can be detected in \u003cspan style=\"font-size: 0.875rem;\"\u003ePerCP\/Cy5.5 channel.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2~8°C for one year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is for research use only.\u003c\/li\u003e\n\u003cli\u003eWhen detecting adherent cells, the suspension cells generated after induction of apoptosis should be collected and detected together with the subsequently collected adherent cells.\u003c\/li\u003e\n\u003cli\u003eMechanical damage caused by digestion of adherent cells should be avoided as much as possible. At the same time, trypsin digestion solution should not contain EDTA as much as possible, because EDTA will affect the binding of Annexin V to phosphatidylserine.\u003c\/li\u003e\n\u003cli\u003eIf trypsin containing EDTA is used, cells should be washed thoroughly after harvesting to ensure that EDTA is removed.\u003c\/li\u003e\n\u003cli\u003eDetect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.\u003c\/li\u003e\n\u003cli\u003eAvoid extended exposure of the samples to direct light to protect the fluorophores from quenching.\u003c\/li\u003e\n\u003cli\u003eFor your safety and health, please wear the lab coat and disposable gloves before the experiments.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Elabscience Biotechnology","offers":[{"title":"200 Assays","offer_id":40441673678906,"sku":"E-CK-A240-200","price":444.95,"currency_code":"USD","in_stock":true},{"title":"100 Assays","offer_id":40441673711674,"sku":"E-CK-A240-100","price":292.95,"currency_code":"USD","in_stock":true},{"title":"50 Assays","offer_id":40441673744442,"sku":"E-CK-A240-50","price":168.95,"currency_code":"USD","in_stock":true},{"title":"20 Assays","offer_id":40441673777210,"sku":"E-CK-A240-20","price":84.95,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/files\/E-CK-A240.jpg?v=1714127051"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0722\/7785\/collections\/Cell_Function_Assays.jpg?v=1769048837","url":"https:\/\/www.msesupplies.com\/collections\/elabscience-cell-function-assays.oembed?page=6","provider":"MSE Supplies","version":"1.0","type":"link"}