Homogenizer HO 4/A (Edmund Buhler, Made in Germany),  MSE Supplies

Homogenizer HO 4/A (Edmund Buhler, Made in Germany)

  • $ 7,94500

Homogenizer HO 4/A

Mechanical crushing of samples with wing knives at very high speed dry or in suspension. Well suited for greasy / brittle, moist / fibrous as well as hard / brittle substances up to Mohs hardness of 5. Some customers have used the HO 4/A for grinding freeze dried leaves for the isolation of chloroplasts.

Basic Equipment:
Basic device incl. protection beaker with safety lock, 2 open-end wrenches for fastening the accessories. Knives and stainless steel blades must be purchased separately. The unique design of these Edmund Bhler knives makes them far easier to clean than the competitions blades.

Left: The Edmund Bhler homogenizer. Note the blades that can be removed for easy cleaning.Right: Traditional homogenizer tips with the blades buried deep within the tip.

Details (click here for spindles, knives, or sample vessels)

Speed range: 1.500 to 35.000 rpm, steplessly adjustable
Timer: adjustable between 0 - 100 sec, min or h / continuous
Sample vessels: Duran glass 10 - 150 ml
Cooling: with dry ice or cooling water flow through the protection beaker
Order No.: 3301 000
Accessory: Spindle, Stainless steel 10 ml
Knife, Stainless steel 10 ml
Sample vessel Duran 10 ml
Spindle, Stainless steel 25 - 150 ml
Knife, Stainless steel 25 ml
Knife, Stainless steel 50 ml
Knife, Stainless steel 100 ml
Knife, Stainless steel 150 ml
Sample vessel, Duran 25 ml
Sample vessel, Duran 50 ml
Sample vessel, Duran 100 ml
Sample vessel, Duran 150 ml

Technical specifications

Electrical supply: 100 - 240 V, 50 / 60 Hz
Dimensions (w x d x h): 320 x 350 x 600 mm
Weight: 20 kg

Cleaning traditional homogenizers is performed by running them in a solution containing detergent. This generally works well enough. Problems arise when high nutrient tissue is flung far up into the tip. This generally leads to bacterial contamination.

Homogenizer accessories
Some glass Teflon and glass-glass Dounce homogenizers

Dounce homogenizers are very easy to clean, but they are prone to shear stress that causes air bubbles, or cavitation, that can damage the material being homogenzied. While many have used Dounce homogenizers for isolating organelles from soft tissue, the HO 4A also appears to be good for this purpose.